HIGD2A (NM_138820) Human Untagged Clone

SKU SC120662

HIGD2A (untagged)-Human HIG1 hypoxia inducible domain family, member 2A (HIGD2A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HIGD2A
• Synonyms: RCF1b
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120662 sequence for NM_138820 edited (data generated by NextGen Sequencing); ATGGCGACTCCCGGCCCTGTGATTCCGGAGGTCCCCTTTGAACCATCGAAGCCTCCAGTC; ATTGAGGGGCTGAGCCCCACTGTTTACAGGAATCCAGAGAGTTTCAAGGAAAAGTTCGTT; CGCAAGACCCGCGAGAACCCGGTGGTACCCATAGGTTGCCTGGCCACGGCGGCCGCCCTC; ACCTACGGCCTCTACTCCTTCCACCGGGGCAACAGCCAGCGCTCTCAGCTCATGATGCGC; ACCCGGATCGCCGCCCAGGGTTTCACGGTCGCAGCCATCTTGCTGGGTCTGGCTGTCACT; GCTATGAAGTCTCGACCCTAA; ; Clone variation with respect to NM_138820.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138820 unedited; GGTCACAATTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACCAGCCGGGAGGCTG; AGGTCGGAGTCCCGATTTTCTCCTGCTGCTGTGGCCCGGACATGGCGACTCCCGGCCCTG; TGATTCCGGAGGTCCCCTTTGAACCATCGAAGCCTCCAGTCATTGAGGGGCTGAGCCCCA; CTGTTTACAGGAATCCAGAGAGTTTCAAGGAAAAGTTCGTTCGCAAGACCCGCGAGAACC; CGGTGGTACCCATAGGTTGCCTGGCCACGGCGGCCGCCCTCACCTACGGCCTCTACTCCT; TCCACCGGGGCAACAGCCAGCGCTCTCAGCTCATGATGCGCACCCGGATCGCCGCCCAGG; GTTTCACGGTCGCAGCCATCTTGCTGGGTCTGGCTGTCACTGCTATGAAGTCTCGACCCT; AAGCCCAGGGTCTGGCCTTGAAAGCTCCGCAGAAATGATTCCAAAACCCAGGGAGCAACC; ACTGGCCCTACCGTGGGACTTACTCCCTCCTCTCCTTTGAGAGGCCCATGTGTCGCTGGG; GAGGAAGTGACCCTTTGTGTAACTGTAACCGAAAGTTTTTTCAAAAATCCTAGATGCTGT; TGTTTGAATGTTACATACTTCTATTTGTGCCACATCTCCCCTCCACTCCCCTGCTTAATA; AACTCTAAAAATCCAAAAAAAAAAAAAAAAAAAACTCGACTCTATATTGCCGCCCGGGCC; ATAGCTGTTTCCTGGACAGATCCCGGGTGCAATCCCTGTGACCCCTCCCCAGGCCTCTCC; TGGCCTTGGAATTCGCACTCCAGTGCCACCAGCCTTGTCCCAATAAAATTAAGTTGCATC; ATTTTGCCTGACTAGGATGCCCTCTTTAAAATTAATGGGTGGAGGGGGGGGGTTTGGGAC; AAGGGGCCAATTGGGAAAAAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138820 unedited; GTTAAAACCCTTTTTTTTTTTGGATTTTTAGAGTTTATTAAGCAGGGGAGTGGAGGGGAG; ATGTGGCACAAATAGAAGTATGTAACATTCAAACAACAGCATCTAGGATTTTTGAAAAAA; CTTTCGGTTACAGTTACACAAAGGGTCACTTCCTCCCCAGCGACACATGGGCCTCTCAAA; GGAGAGGAGGGAGTAAGTCCCACGGTAGGGCCAGTGGTTGCTCCCTGGGTTTTGGAATCA; TTTCTGCGGAGCTTTCAAGGCCAGACCCTGGGCTTAAGGTCGAGACTTCATAGCAGTGAC; AGCCAGACCCAGCAAGATGGCTGCGACCGTGAAACCCTGGGCGGCGATCCGGGTGCGCAT; CATGAGCTGAGAGCGCTGGCTGTTGCCCCGGTGGAAGGAGTAGAGGCCGTAAGTGAGGGC; GGCCGCCGTGGCCAGGCAACCTATGGGTACCACCGGGTTCTCGCGGGTCTTGCGAACGAA; CTTTTCCTTGAAACTCTCTGGATTCCTGTAAACAGTGGGGCTCAGCCCCTCAATGACTGG; AGGCTTCGATGGTTCAAAGGGGACCTCCGGAATCACAGGGCCGGGAGTCGCCATGTCCGG; GCCACAGCAGCAGGAGAAAATCGGGACTCCGACCTCAGCCTCCCGGCTGGTGCCGAATTC; GCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGC; TTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGGGGTTA; TTACGACATTTTGGAAAGTCCCCGTGATTTTGGTGCCAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_138820
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138820.2, NP_620175.1
• RefSeq Size: 628 bp
• RefSeq ORF: 321 bp
• Locus ID: 192286
• UniProt ID: Q9BW72
• Cytogenetics: 5q35.2
• Domains: HIG_1_N
• Protein Families: Transmembrane
• Summary: The protein encoded by this gene is a subunit of the cytochrome c oxidase complex (complex IV), which is the terminal enzyme in the mitochondrial respiratory chain. The encoded protein is an inner mitochondrial membrane protein and is a functional ortholog of the yeast respiratory supercomplex factor 1 (Rcf1). In mouse, the orthologous protein enhances cell survival under conditions of hypoxia. [provided by RefSeq, Sep 2016]