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EDARADD (NM_080738) Human Untagged Clone

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SKU
SC120378
EDARADD (untagged)-Human EDAR-associated death domain (EDARADD), transcript variant B
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EDARADD
Synonyms ECTD11A; ECTD11B; ED3; EDA3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC120378 sequence for NM_080738 edited (data generated by NextGen Sequencing)
ATGGCTTCACCGGACGACCCTCTGCGCGCAGATCATATGGTAAAGGAACCAGTGGAAGAC
ACAGACCCTAGCACTTTATCCTTTAATATGTCAGACAAATATCCCATTCAAGATACGGAA
CTCCCTAAAGCTGAAGAATGTGATACAATTACTTTGAACTGCCCACGAAATTCAGATATG
AAAAATCAGGGAGAAGAAAATGGCTTTCCAGATAGCACTGGAGATCCTCTTCCAGAGATC
AGCAAGGACAACTCCTGCAAAGAAAACTGTACTTGTTCCTCCTGCTTGCTCCGGGCCCCC
ACCATAAGTGACTTGCTCAATGATCAGGACTTACTAGATGTGATCAGGATAAAGCTGGAT
CCGTGTCACCCAACGGTGAAAAACTGGAGGAATTTTGCAAGCAAATGGGGGATGTCCTAT
GACGAATTGTGCTTCCTGGAGCAGAGGCCACAGAGCCCCACCTTGGAGTTCTTGCTCCGG
AACAGTCAGAGGACGGTGGGCCAGCTGATGGAGCTCTGCAGGCTCTACCACAGGGCCGAC
GTGGAGAAGGTTCTGCGCAGGTGGGTGGACGAGGAGTGGCCCAAGCGGGAGCGTGGAGAC
CCCTCCAGGCACTTCTAG

Clone variation with respect to NM_080738.3
339 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_080738 unedited
TAGGCGGCCGCGACATCGGCACGACCCGGGACACTCGGGGCCCCGCCTTGCGTCCCAGCC
CCGGGTCGCGGCACCCCGGCTCCCGCCCCGCGCCTCTGGAGGGAGGTACCCGAGGGACCG
CGCAGCGAAGGGGCTTTGCTAATTGCCAAAGCAGGAAGTGAGCTCGTGGAAGAAGCGAAG
GAGGAGAAGAAGAAAGGAGGGGAAGGCGGAGGAGGGCAGGGGCGCGCAGAGCCACGGTTT
GCTCCAGGCGCGTCGGAACCGCAGGACTTTTCATCCCCGTGGTCCCACGGTCCTCCCGCG
CCCCGGAGGCCTGCCAGCCCCGCTCGGACGCTCGTTTGCCCCTAACCCGCCGCCATGGCT
TCACCGGACGACCCTCTGCGCGCAGATCATATGGTAAAGGAACCAGTGGAAGACACAGAC
CCTAGCACTTTATCCTTTAATATGTCAGACAAATATCCCATTCAAGATACGGAACTCCCT
AAAGCTGAAGAATGTGATACAATTACTTTGAACTGCCCACGAAATTCAGATATGAAAAAT
CAGGGAGAAGAAAATGGCTTTCCAGATAGCACTGGAGATCCTCTTCCAGAGATCANNGCA
GGACACTCCTGNCAAGAAACTGTACTNTGTCCTCCTGCTTTGCTCNGGCCCCCACCATAA
GTGACTTGCTCAATGATCANGACTTACTAGATGTGATCANGATAAAGCTGGATCCGTGTC
ACCNCACGGTGAAAAACTGGNAGAATTTTTGCAGCAATGGGGGATGTCCTATGACGAATT
GTGCTTCCTGNAGCAGAGGCACAGACCCCCACTTGGGAGTTCTTTGCTCGGACAATCANA
GGACGGTGGCCCAGCTGATGGAGCTCTGCAGGCTCTACACAGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_080738 unedited
NNNNNGGGTACATCTAAGNAACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTTTT
TTTTTCTGGGACGGACTCTCACTCTGTCTCCAGAACTGGTGTGCAGTGGCGCAATCTCGG
CTCACTGCAACCTGCTTCTGTAGAAGTTCTAAGGAAGAGGTGCGAACTCCACGGCGGTGG
GCAAGGGTAGCTAACTAGCAGCGGCCCCTACAAATGTTGCTCGGGGGTCTTGAGCTGCCG
GAGTTGACACAAGGGGAGGGGTCTAATTAGCCACCAGGTGACTGAAGGGCTTGCCTGCCC
ACAGCTTACTTGGCTGGGGGGTTTCTGAAGTTCCTGCCGGCAAACTTAGCCTTGCTGCCC
AGCTCCTCTTCAATTCTGAGGAGCTGGTTGTACTTGGCCAAGCGCTCAGATCAGCAAGGG
GCACCAGTCTTGAGCTGCCCAGGTCACAGCCCCACCACCAGGTCAGTGATGAAGGTATTT
TCAGTCTCCCCAGAATGATGAGGCACCATGACACACCAACCATTGGCCTGGGCCAGCTTG
CACGCCTGAAGGGACTCAGTCACAGAGCGAATCTGGTTCACTTTGAGCAGGAGGCAGTTG
CACTTCTTCTCATTCACGGCCGAGGCTGTCCTCTTTGGGTTGGTCACTCTGAGATCATCC
TCCACTACCTGGATTCCTGCACTGGCCGTGAACTTCTGCCAAGCTCCCCAGTCATCCTGG
TCAAAGGGATCTTCAGTAGACACCACTGGGTAGTTTTTGATGAAGGACTTGTACAGGTCA
GCCAGACCGTCAGGTGAGATGTACCTGGTGGGGGTCGTCGAGAAACTTGAATTCCCAGTC
ATACTTTCCAGACCTGAAGAACTCGGAGGCCTCTACGTCCT
Restriction Sites NotI-NotI |
ACCN NM_080738
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080738.2, NP_542776.1
RefSeq Size 2891 bp
RefSeq ORF 618 bp
Locus ID 128178
UniProt ID Q8WWZ3
Cytogenetics 1q42.3-q43
Domains enolase
Protein Families Druggable Genome
Summary This gene was identified by its association with ectodermal dysplasia, a genetic disorder characterized by defective development of hair, teeth, and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein, and is found to interact with EDAR, a death domain receptor known to be required for the development of hair, teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR, this protein acts as an adaptor, and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008
Transcript Variant: This variant (B) differs in the 5' region compared to variant A. It encodes an isoform (B) that contains a distinct and shorter N-terminus, as compared to isoform (A).
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Documents
Resources
"NM_080738" in other vectors (6)
SKU Description Size Price
RC219198 EDARADD (Myc-DDK-tagged)-Human EDAR-associated death domain (EDARADD), transcript variant B 10 ug
$300.00
RC219198L1 Lenti ORF clone of Human EDAR-associated death domain (EDARADD), transcript variant B, Myc-DDK-tagged 10 ug
$600.00
RC219198L2 Lenti ORF clone of Human EDAR-associated death domain (EDARADD), transcript variant B, mGFP tagged 10 ug
$600.00
RC219198L3 Lenti ORF clone of Human EDAR-associated death domain (EDARADD), transcript variant B, Myc-DDK-tagged 10 ug
$600.00
RC219198L4 Lenti ORF clone of Human EDAR-associated death domain (EDARADD), transcript variant B, mGFP tagged 10 ug
$600.00
RG219198 EDARADD (tGFP-tagged) - Human EDAR-associated death domain (EDARADD), transcript variant B 10 ug
$500.00

file-alt Citations

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