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SLC8A3 (NM_058240) Human Untagged Clone

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SKU
SC120250
SLC8A3 (untagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 3 (SLC8A3), transcript variant b
  $847.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SLC8A3
Synonyms NCX3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_058240, the custom clone sequence may differ by one or more nucleotides


ATGGCGTGGTTAAGGTTGCAGCCTCTCACCTCTGCCTTCCTCCATTTTGGGCTGGTTACCTTTGTGCTCT
TCCTGAATGGTCTTCGAGCAGAGGCTGGTGGCTCAGGGGACGTGCCAAGCACAGGGCAGAACAATGAGTC
CTGTTCAGGGTCATCGGACTGCAAGGAGGGTGTCATCCTGCCAATCTGGTACCCGGAGAACCCTTCCCTT
GGGGACAAGATTGCCAGGGTCATTGTCTATTTTGTGGCCCTGATATACATGTTCCTTGGGGTGTCCATCA
TTGCTGACCGCTTCATGGCATCTATTGAAGTCATCACCTCTCAAGAGAGGGAGGTGACAATTAAGAAACC
CAATGGAGAAACCAGCACAACCACTATTCGGGTCTGGAATGAAACTGTCTCCAACCTGACCCTTATGGCC
CTGGGTTCCTCTGCTCCTGAGATACTCCTCTCTTTAATTGAGGTGTGTGGTCATGGGTTCATTGCTGGTG
ATCTGGGACCTTCTACCATTGTAGGGAGTGCAGCCTTCAACATGTTCATCATCATTGGCATCTGTGTCTA
CGTGATCCCAGACGGAGAGACTCGCAAGATCAAGCATCTACGAGTCTTCTTCATCACCGCTGCTTGGAGT
ATCTTTGCCTACATCTGGCTCTATATGATTCTGGCAGTCTTCTCCCCTGGTGTGGTCCAGGTTTGGGAAG
GCCTCCTCACTCTCTTCTTCTTTCCAGTGTGTGTCCTTCTGGCCTGGGTGGCAGATAAACGACTGCTCTT
CTACAAATACATGCACAAAAAGTACCGCACAGACAAACACCGAGGAATTATCATAGAGACAGAGGGTGAC
CACCCTAAGGGCATTGAGATGGATGGGAAAATGATGAATTCCCATTTTCTAGATGGGAACCTGGTGCCCC
TGGAAGGGAAGGAAGTGGATGAGTCCCGCAGAGAGATGATCCGGATTCTCAAGGATCTGAAGCAAAAACA
CCCAGAGAAGGACTTAGATCAGCTGGTGGAGATGGCCAATTACTATGCTCTTTCCCACCAACAGAAGAGC
CGTGCCTTCTACCGTATCCAAGCCACTCGTATGATGACTGGTGCAGGCAATATCCTGAAGAAACATGCAG
CAGAACAAGCCAAGAAGGCCTCCAGCATGAGCGAGGTGCACACCGATGAGCCTGAGGACTTTATTTCCAA
GGTCTTCTTTGACCCATGTTCTTACCAGTGCCTGGAGAACTGTGGGGCTGTACTCCTGACAGTGGTGAGG
AAAGGGGGAGACATGTCAAAGACCATGTATGTGGACTACAAAACAGAGGATGGTTCTGCCAATGCAGGGG
CTGACTATGAGTTCACAGAGGGCACGGTGGTTCTGAAGCCAGGAGAGACCCAGAAGGAGTTCTCCGTGGG
CATAATTGATGACGACATTTTTGAGGAGGATGAACACTTCTTTGTAAGGTTGAGCAATGTCCGCATAGAG
GAGGAGCAGCCAGAGGAGGGGATGCCTCCAGCAATATTCAACAGTCTTCCCTTGCCTCGGGCTGTCCTAG
CCTCCCCTTGTGTGGCCACAGTTACCATCTTGGATGATGACCATGCAGGCATCTTCACTTTTGAATGTGA
TACTATTCATGTCAGTGAGAGTATTGGTGTTATGGAGGTCAAGGTTCTGCGGACATCAGGTGCCCGGGGT
ACAGTCATCGTCCCCTTTAGGACAGTAGAAGGGACAGCCAAGGGTGGCGGTGAGGACTTTGAAGACACAT
ATGGGGAGTTGGAATTCAAGAATGATGAAACTGTGAAAACCATAAGGGTTAAAATAGTAGATGAGGAGGA
ATACGAAAGGCAAGAGAATTTCTTCATTGCCCTTGGTGAACCGAAATGGATGGAACGTGGAATATCAGCG
CTCCTGTTATCTCCAGACAGGAAGCTGACTATGGAAGAAGAGGAGGCCAAGAGGATAGCAGAGATGGGAA
AGCCAGTATTGGGTGAACACCCCAAACTAGAAGTCATCATTGAAGAGTCCTATGAGTTCAAGACTACGGT
GGACAAACTGATCAAGAAGACAAACCTGGCCTTGGTTGTGGGGACCCATTCCTGGAGGGACCAGTTCATG
GAGGCCATCACCGTCAGTGCAGCAGGGGATGAGGATGAGGATGAATCCGGGGAGGAGAGGCTGCCCTCCT
GCTTTGACTACGTCATGCACTTCCTGACTGTCTTCTGGAAGGTGCTGTTTGCCTGTGTGCCCCCCACAGA
GTACTGCCACGGCTGGGCCTGCTTCGCCGTCTCCATCCTCATCATTGGCATGCTCACCGCCATCATTGGG
GACCTGGCCTCGCACTTCGGCTGCACCATTGGTCTCAAAGATTCAGTCACAGCTGTTGTTTTCGTGGCAT
TTGGCACCTCTGTCCCAGATACGTTTGCCAGCAAAGCTGCTGCCCTCCAGGATGTATATGCAGACGCCTC
CATTGGCAACGTGACGGGCAGCAACGCCGTCAATGTCTTCCTGGGCATCGGCCTGGCCTGGTCCGTGGCC
GCCATCTACTGGGCTCTGCAGGGACAGGAGTTCCACGTGTCGGCCGGCACACTGGCCTTCTCCGTCACCC
TCTTCACCATCTTTGCATTTGTCTGCATCAGCGTGCTCTTGTACCGAAGGCGGCCGCACCTGGGAGGGGA
GCTTGGTGGCCCCCGTGGCTGCAAGCTCGCCACAACATGGCTCTTTGTGAGCCTGTGGCTCCTCTACATA
CTCTTTGCCACACTAGAGGCCTATTGCTACATCAAGGGGTTCTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_058240 unedited
GTCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTGCAATAGAA
GCCAGTGGCTAAGTCTCGTGTATGGCGTGGTTAAGGTTGCAGCCTCTCACCTCTGCCTTC
CTCCATTTTGGGCTGGTTACCTTTGTGCTCTTCCTGAATGGTCTTCGAGCAGAGGCTGGT
GGCTCAGGGGACGTGCCAAGCACAGGGCAGAACAATGAGTCCTGTTCAGGGTCATCGGAC
TGCAAGGAGGGTGTCATCCTGCCAATCTGGTACCCGGAGAACCCTTCCCTTGGGGACAAG
ATTGCCAGGGTCATTGTCTATTTTGTGGCCCTGATATACATGTTCCTTGGGGTGTCCATC
ATTGCTGACCGCTTCATGGCATCTATTGAAGTCATCACCTCTCAAGAGAGGGAGGTGACA
ATTAAGAAACCCAATGGAGAAACCAGCACAACCACTATTCGGGTCTGGAATGAAACTGTC
TCCAACCTGACCCTTATGGCCCTGGGTTCCTCTGCTCCTGAGATACTCCTCTCTTTAATT
GAGGTGTGTGGTCATGGGTTCATTGCTGGTGATCTGGGACCTTCTACCATTGTAGGGAGT
GCAGCCTTCAACATGTTCATCATCATTGGCATCTGTGTCTACGTGATCCCAGACGGAGAG
ACTCGCAAGATCAAGCATCTACGAGTCTTCTTCATCACCGCTGCTTGGAGTATCTTTGCC
TACATCTGGCTCTATATGATTCTGGCAGTCTTCTNCCCTGGTGTGGTCCANGTTTTGGAA
GGCCTNCTCACTCTCTTCTTCTTTTCAGTGTGTGTNCCTCTGGCCCTGGTGGCAGATAAC
GACTGCTCTTCTACAATACATGCACAAAAAGTACCGCACAGACAACACCCGAGAATTATC
TAGAGACAAAGGTGACCACCCTAAGGCATTGANAGGATGGAAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_058240 unedited
AAATCTTGNCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTATGTAGA
GAGATATAGTATAGCTTTTATTTTTTTTTTGAACCAACCCAACATTTCCACTGGCAACTG
TCAACATAGCTTATGACATGAGCACTGTTTCTTTTTTAATTATTTTTTTCTTAAAAAATG
TGTTTAAAATTAACAGGTGTTTTTTAATTTCTTCCCCCCCCCCCTTAGAAAATGTATTTA
TGTCAATGCAGAAAGCCTAAAGATCCGTGGGCAGTTTCGTGATGACATCTTATGTCTGTT
TATTTGTGTGTTTGTGTGCGTGTTTTAAAGCCAGGTCCTTCCCACATTCGTTGAGAAAAG
GTCTGTAGGGCACGGAGATCTCTTTCTCCAAAAACCCAGGCTCCAAGGGATTATGACAAC
TAAGATTCCTCTTACTGGAGCAAGAACTACCCCATAGGCTGGAAAAATAGCTTCATGAAG
TTTAAGAACAAGCAGGAACATGGGAGAAATGAGGAATTCCAGTTCCTATCTTGTAAAAAT
GCTTTCATATGTGACCTGAGGGACCAGGAGAGGTGAAGGGAGCCCCTGGTTCTCTTATAA
AATCTCTAAGTTTTTACACCACTTCAAGAAATATAGTCAGCCCTCTTTGTATAGAGCATT
AGGAAGCAAGAGCGTGGTGAAGCTGACATTTCTCAGGCACATTGACATTTAAAATACANA
CTTTTCTCTTAAAGATCTCACTGGTTGGACATCTCTTCTCTTTCTGCCTCAGTACAAAGC
CAAAGATAATCAAGAGGCCTGTATCCCTTTTTGTTCTTCAGTTAGCCCTTTGTCGTNCAC
ACCATTCCTATTTTTGTCCATCTCTTCTATTGGCCGGTCTTCCTGCAGAAGTATCACAAG
TTGACACTGACCCACCAGACCCCAGATTGGAAAG
Restriction Sites NotI-NotI |
ACCN NM_058240
Insert Size 4650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_058240.2, NP_489479.1
RefSeq Size 5259 bp
RefSeq ORF 2775 bp
Locus ID 6547
UniProt ID P57103
Cytogenetics 14q24.2
Domains Calx-beta, Na_Ca_Ex
Protein Families Transmembrane
Protein Pathways Calcium signaling pathway
Summary This gene encodes a member of the sodium/calcium exchanger integral membrane protein family. Na+/Ca2+ exchange proteins are involved in maintaining Ca2+ homeostasis in a wide variety of cell types. The protein is regulated by intracellular calcium ions and is found in both the plasma membrane and intracellular organellar membranes, where exchange of Na+ for Ca2+ occurs in an electrogenic manner. Alternative splicing has been observed for this gene and multiple variants have been described. provided by RefSeq, Aug 2013
Transcript Variant: This variant (b) uses an alternate in-frame splice site in the mid coding region, compared to variant c. The encoded isoform (B) is shorter than isoform C.
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Documents
Resources
"NM_058240" in other vectors (4)
SKU Description Size Price
RC221932 SLC8A3 (Myc-DDK-tagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 3 (SLC8A3), transcript variant b 10 ug
$846.00
RC221932L3 Lenti ORF clone of Human solute carrier family 8 (sodium/calcium exchanger), member 3 (SLC8A3), transcript variant b, Myc-DDK-tagged 10 ug
$1,146.00
RC221932L4 Lenti ORF clone of Human solute carrier family 8 (sodium/calcium exchanger), member 3 (SLC8A3), transcript variant b, mGFP tagged 10 ug
$1,146.00
RG221932 SLC8A3 (tGFP-tagged) - Human solute carrier family 8 (sodium/calcium exchanger), member 3 (SLC8A3), transcript variant b 10 ug
$1,046.00

file-alt Citations

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