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PRPH2 (NM_000322) Human Untagged Clone

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SKU
SC119972
PRPH2 (untagged)-Human peripherin 2 (retinal degeneration, slow) (PRPH2)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRPH2
Synonyms AOFMD; AVMD; CACD2; DS; MDBS1; PRPH; rd2; RDS; RP7; TSPAN22
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC119972 sequence for NM_000322 edited (data generated by NextGen Sequencing)
ATGGCGCTACTGAAAGTCAAGTTTGACCAGAAGAAGCGGGTCAAGTTGGCCCAAGGGCTC
TGGCTCATGAACTGGTTCTCCGTGTTGGCTGGCATCATCATCTTCAGCCTAGGACTGTTC
CTGAAGATTGAACTCCGAAAGAGGAGCGATGTGATGAATAATTCTGAGAGCCATTTTGTG
CCCAACTCATTGATAGGGATGGGGGTGCTATCCTGTGTCTTCAACTCGCTGGCTGGGAAG
ATCTGCTACGACGCCCTGGACCCAGCCAAGTATGCCAGATGGAAGCCCTGGCTGAAGCCG
TACCTGGCTATCTGTGTCCTCTTCAACATCATCCTCTTCCTTGTGGCTCTCTGCTGCTTT
CTGCTTCGGGGCTCGCTGGAGAACACCCTGGGCCAAGGGCTCAAGAACGGCATGAAGTAC
TACCGGGACACAGACACCCCTGGCAGGTGTTTCATGAAGAAGACCATCGACATGCTGCAG
ATCGAGTTCAAATGCTGCGGCAACAACGGTTTTCGGGACTGGTTTGAGATTCAGTGGATC
AGCAATCGCTACCTGGACTTTTCCTCCAAAGAAGTCAAAGATCGAATCAAGAGCAACGTG
GATGGGCGGTACCTGGTGGACGGCGTCCCTTTCAGCTGCTGCAATCCTAGCTCGCCACGG
CCCTGCATCCAGTATCAGATCACCAACAACTCAGCACACTACAGTTACGACCACCAGACG
GAGGAGCTCAACCTGTGGGTGCGTGGCTGCAGGGCTGCCCTGCTGAGCTACTACAGCAGC
CTCATGAACTCCATGGGTGTCGTCACGCTCCTCATTTGGCTCTTCGAGGTGACCATTACA
ATTGGGCTGCGCTACCTACAGACGTCGCTGGATGGTGTGTCCAACCCCGAGGAATCTGAG
AGCGAGAGCGAGGGCTGGCTGCTGGAGAAGAGCGTGCCGGAGACCTGGAAGGCCTTTCTG
GAGAGTGTGAAGAAGCTGGGCAAGGGCAACCAGGTGGAAGCCGAGGGCGCAGGCGCAGGC
CAGGCCCCAGAGGCTGGCTGA

Clone variation with respect to NM_000322.4
318 t=>c;910 c=>g;929 g=>a;1013 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000322 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCATGGCGCTACT
GAAAGTCAAGTTTGACCAGAAGAAGCGGGTCAAGTTGGCCCAGGGCTCTGGCTCATGAAC
TGGTTCTCCGTGTTGGCTGGCATCATCATCTTCAGCCTAGGACTGTTCCTGAAGATTGAA
CTCCGAAAGAGGAGCGATGTGATGAATAATTCTGAGAGCCATTTTGTGCCCAACTCATTG
ATAGGGATGGGGGTGCTATCCTGTGTCTTCAACTCGCTGGCTGGGAAGATCTGCTACGAC
GCCCTGGACCCAGCCAAGTATGCCAGATGGAAGCCCTGGCTGAAGCCGTACCTGGCTATC
TGTGTCCTCTTCAACATCATCCTCTTCCTTGTGGCTCTCTGCTGCTTTCTGCTTCGGGGC
TCGCTGGAGAACACCCTGGGCCAAGGGCTCAAGAACGGCATGAAGTACTACCGGGACACA
GACACCCCTGNGCAGGTGTTTCATGAAGAAGACCATCGACATGCCTGCAGATCGAGTTCA
AATGCTGCGGAACAACGGTTTTCGGGAACTGGTTTGGNAATTCAGTGGATCAGCAATCGC
TACCTGNACTTTTCCTCCAAAGAGTCAAAGAGTTGCAGGATGAGCAAGCTGGCAGTGTCT
TTTGGGTGNGAGAGGTANAAACATGCGGGAGCTCAGACATTNTTGGAACCAGAACTACCA
CCCCAGAGGCCACCCTGTGGGGTTCCCATCCTGGAGGGGAAAAGCTGGCACCAGCTCTTG
CTCTTCACATTCTTCCCAGGACCCCAGACTACGTCATAGACACCNGGTGTGGGGGCGCCT
CTTGGGGGGGACGGCGTCTTTTAGTGCTGCATCTACTCGCACGCCTGATCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000322 unedited
TTTTNNAATTTTTCTTGAACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTCCATACAGNATGAATTTTAATTATCTATTGACAGCTGTTAACAG
CATAGTTACACTTGGGGGCCTAAAGCAGCACTTTCACCGGGGTTCTCAGCACTCTTGAAA
GATAGCAAGAGCTCAGGGACCTTTGCCAAAAATGGCTGGCTCAGCATTTTTTCAGTATGG
ATCATTCCTTGGCCTCAACGAGATTCAAAAATCTAATTAAACTTGGGACCAACGTCTGAC
AGGGTGTCCAAACATCTTGGATCTGGTCTTCTGCTTGGGGGAATTAAGCCCAGAACATAC
CCCAGCAGCACCAGAAGTCTTATTTTCTCTACACTAAAGTTCTTAGGACAGCAAAGACCC
CAACTCAGTCCAAAGTACCTATTCCCAAACACCATCGTCAAAGCAGAGGTAAGTATGGAC
TGTTCCCTTTCTGGTTTTGCCTTTTGCCCTGGGCTCCAGCCAGTAAGAACGAGCTCTCTG
AATGTCATCCAACTGGAGGAATATGCTGGGCCCTGCCTTGGTTGGGCACCAAGGGCTTGG
GAAATCACCAAATCTTCTTTTCCTTCCCCTGAAAACTTTTTTTTCTAAAGAAAGGGAAAC
CTCCTGTTTTGGCTGCCTACGAAAAAGGGCCTTTCCAAAAGATTTCCTTTGATGCCAAAG
CATTCCAAAAATAGGGTTTTTAAAAAGGGTTCTTGGGGGGAAAAAACGAGAAAACCCCCA
AAGAATTCTTGCCCCTTGTGGGGAACATAATGGTGCCCTGGGGCTGGAGGCACCTTGGGG
GGGGCCCTGGCCCCCAAAACGAGGCCGGGTCCAAAGGGGAACCAGGGGAAGGGGCGCCTT
TCCAAGGGGGAAAACTACCCCTGTTTCCCGAAACCAGGAAAACCGCCTTGGGGGGG
Restriction Sites NotI-NotI |
ACCN NM_000322
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000322.2, NP_000313.1
RefSeq Size 2974 bp
RefSeq ORF 1041 bp
Locus ID 5961
UniProt ID P23942
Cytogenetics 6p21.1
Domains transmembrane4
Protein Families Druggable Genome, Transmembrane
Protein Pathways Amyotrophic lateral sclerosis (ALS)
Summary The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein found in the outer segment of both rod and cone photoreceptor cells. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. This protein is essential for disk morphogenesis. Defects in this gene are associated with both central and peripheral retinal degenerations. Some of the various phenotypically different disorders are autosomal dominant retinitis pigmentosa, progressive macular degeneration, macular dystrophy and retinitis pigmentosa digenic. provided by RefSeq, Jul 2008
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"NM_000322" in other vectors (6)
SKU Description Size Price
RC210357 PRPH2 (Myc-DDK-tagged)-Human peripherin 2 (retinal degeneration, slow) (PRPH2) 10 ug
$457.00
RC210357L1 Lenti ORF clone of Human peripherin 2 (retinal degeneration, slow) (PRPH2), Myc-DDK-tagged 10 ug
$757.00
RC210357L2 Lenti ORF clone of Human peripherin 2 (retinal degeneration, slow) (PRPH2), mGFP tagged 10 ug
$757.00
RC210357L3 Lenti ORF clone of Human peripherin 2 (retinal degeneration, slow) (PRPH2), Myc-DDK-tagged 10 ug
$757.00
RC210357L4 Lenti ORF clone of Human peripherin 2 (retinal degeneration, slow) (PRPH2), mGFP tagged 10 ug
$757.00
RG210357 PRPH2 (tGFP-tagged) - Human peripherin 2 (retinal degeneration, slow) (PRPH2) 10 ug
$489.00 $657.00

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