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SRD5A2 (NM_000348) Human Untagged Clone

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SKU
SC119922
SRD5A2 (untagged)-Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SRD5A2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_000348 edited
CGGCACGAGGCAGCGGCCACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAG
AGCCCAGTGCTGGCAGGCAGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTC
GCGAAGCCCTCCGGCTACGGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTG
CCAGCCCGCGCCGCCTGGTTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATC
CTCGCCCGGCAGCCCCTCTCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTC
TGCCTACATTACTTCCACAGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTAT
CCAGCTATACTCATTCTCAGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGC
TACTATCTGATTTACTGTGCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGC
TTGGGTGTCTTCTTATTTATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTG
CGCCAGCTCAGGAAGCCTGGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACG
TATGTTTCTGGAGCCAATTTCCTCGGTGAGATCATTGAATGGATCGGCTATGCCCTGGCC
ACTTGGTCCCTCCCAGCACTTGCATTTGCATTTTTCTCACTTTGTTTCCTTGGGCTGCGA
GCTTTTCACCACCATAGGTTCTACCTCAAGATGTTTGAGGACTACCCCAAATCTCGGAAA
GCCCTTATTCCATTCATCTTTTAAAGGAACCAAATTAAAAAGGAGCAGAGCTCCCACAAT
GCTGATGAAAACTGTCAAGCTGCTGAAACTGTAATTTTCATGATATAATAGTCCCGTATA
TATGTAATAGTAGGTCTCCTGGCGTTCTGCCAGCTGGCCTGGGGATTCTGAGTGGTGTCT
GCTTAGAGTTTACTCCTACCCTTCCAGGGACCCCTATCCTGATCCCCAACTGAAGCTTCA
AAAAGCCACTTTTCCAAATGGCGACAGTTGCTTCTTAGCTATTGCTCTGAGAAAGTACAA
ACTTCTCCTATGTCTTTCACCGGGCAATCCAAGTACATGTGGCTTCATACCCACTCCCTG
TCAATGCAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000348 unedited
TCGCATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCAGCGGCC
ACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAGAGCCCAGTGCTGGCAGGC
AGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTCGCGAAGCCCTCCGGCTAC
GGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTGCCAGCCCGCGCCGCCTGG
TTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATCCTCGCCCGGCAGCCCCTC
TCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTCTGCCTACATTACTTCCAC
AGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTATCCAGCTATACTCATTCTC
AGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGCTACTATCTGATTTACTGT
GCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGCTTGGGTGTCTTCTTATTT
ATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTGCGCCAGCTCAGGAAGCCT
GGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACGTATGTTTCTGGAGCCAAT
TTCCTCGGTGAGATCATTGAATGGNATCGCTATGCCCTGGCCACNTGGTCCCTCCNAGCA
CTTGCATTTGCATNTTTCTCACTTTTGTTTCTTGGGCTGCGAGCTTTTCACCACCATAAG
TTCTACCTCAAGATGTTGAGGGACTACCCAATCTCGAAAGCCCCTATNCATTCATCTTTA
AGGAACCANTTAAAAAGAGCAGACTCCNACATGCTGATGAAACTGTCAGCTGCTGAACTG
GNATTTCATGAATATAGTCCGTTATATGTAAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000348 unedited
GCCGCAATCTAGAGTCGAGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGGA
TTAAAAGGTTTTTAATGAACAACAAAAACACTTATTTATATGATGGCAATTGGCATTTTT
CAGTAATCTACATCATCCTAAAACCTTTAAAGTTTCCTATGGGACTTATATAGGGAGTTT
CTGGGCTTAGTCCCACTGGGGCTCATTTGTCAAAACAGTTGGCTAAATATTGACAATGCA
TTCCGCAAACATAGGCCTGAAAAAACAAATATTTCCATGTATTCCAATTACAAGCGTTCG
GCCCCTTCCTTAAAGAGTCCATATCTCAGCACTCATGCTGGGGGGACCCCTTCACAAAAG
TTGGCAAACTCAAAGGCTTACTACCTACGTAGTTCCAGTTCTGGGGTGGAAATGTGGGCT
CTGTATGTTCAAATCCTCCAACTTTTTAAAGGAACCTAAAATGCTAGAGTTTTATGAAGG
AATCTCCTGATTTTAAAGTTTTTGAACCTTTGGAAAAAAAAAAAAACCTTTTGGTCTGAC
CCAAACCAACCAGGTTTTTTGCCAAAACGTGCCCCTTTGTGTGCAATTTTCAGCCTCTGC
CTGCAGGTCCTTTGAGGAAGGCATTCAGGCCGCCCTTAGGAAACACCTTCTTGACACGGC
CCGAAAATTCAAGGAAAACCTCCTTAAAGGAAATTTTTTTGGGGCTCTCGCGTCCCTCAT
TTGGCGGGGACCTCGGGGGGGGGCAACACCCCTGCCACCCTATCATATACCCTCCCGCCC
TACCCCCACCATTTTCGGCCTCCGCCCCCCCCCCCCACACACCTACGTTCCCCCCGCCTT
TGCAAATTGTTTCCTTCCCCGCGGACCCCCACAGCCATTCGCGTCCCTCCCTTTTCCACA
CACCCCACGTTAGTCCTTTTTGTCTCCGCGTCCATATATTGCCCGTCCAACTATCCGTCA
CTATTATTGGCCTCCGGTCCCCCCCTGATTACATATTCTCTTATCACAACCTGCGTCGCC
CTCACGAGGGTCGTTGTTGTCGCTTAGCCAACTCCATTTAGGTGCTACTGATAAA
Restriction Sites NotI-NotI |
ACCN NM_000348
Insert Size 2550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000348.2, NP_000339.2
RefSeq Size 2464 bp
RefSeq ORF 765 bp
Locus ID 6716
UniProt ID P31213
Cytogenetics 2p23.1
Domains Steroid_dh
Protein Families Druggable Genome, Transmembrane
Protein Pathways Androgen and estrogen metabolism, Prostate cancer
Summary This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). provided by RefSeq, Jul 2008
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Documents
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"NM_000348" in other vectors (6)
SKU Description Size Price
RC213025 SRD5A2 (Myc-DDK-tagged)-Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2) 10 ug
$289.00 $300.00
RC213025L1 Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), Myc-DDK-tagged 10 ug
$600.00
RC213025L2 Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), mGFP tagged 10 ug
$600.00
RC213025L3 Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), Myc-DDK-tagged 10 ug
$600.00
RC213025L4 Lenti ORF clone of Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), mGFP tagged 10 ug
$600.00
RG213025 SRD5A2 (tGFP-tagged) - Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2) 10 ug
$489.00 $500.00

file-alt Citations

Novel non-synonymous and synonymous gene variants of SRD5A2 in patients with 46,XY-DSD and DSD-free subjects
Ramos
PLoS One (2025) 20 (3), e0316497 DOI: 10.1371/journal.pone.0316497
Cited by 1
AI Summary: OriGene’s pCMV6-XL4 plasmid containing full-length human SRD5A2 cDNA (clone #SC119922) was used in this study to express wild-type and mutant SRD5A2 proteins in HEK293 cells, enabling functional analysis of gene variants associated with 46,XY-DSD.
Snippet: s were performed using Chromas software. Mutagenesis and enzyme activity of wild-type and mutant p.I161L-SRD5A2 Human embryonic kidney 293 (HEK293) cells were transfected with the pCMV6-XL4 plasmid (OriGene Technologies Inc., Rockville, MD, USA), which contained the full-length human SRD5A2 cDNA (clone #SC119922, Rockville, MD, USA). The plasmid was mutated using two mutagenic primers (5´-gcgcaa
FigureFigureFigure
Mutational analysis of SRD5A2: From gene to functional kinetics in individuals with steroid 5α-reductase 2 deficiency
Ramos, Vilchis, Chávez et al
J. Steroid Biochem. Mol. Biol. (2020) 200, 105691 DOI: 10.1016/j.jsbmb.2020.105691
Cited by 16
AI Summary: OriGene’s pCMV6-XL4 plasmid containing full-length human SRD5A2 cDNA (clone #SC119922) was used as the template to generate SRD5A2 gene mutants for functional and structural analysis in this study.
Snippet: 2.3. Site-Directed Mutagenesis of Human SRD5A2. The pCMV6-XL4 plasmid (OriGene Technologies Inc., Rockville, MD, USA) that contains full-length human SRD5A2 cDNA (clone #SC119922, Rockville, MD, USA) was used to construct the mutants
Hamster SRD5A3 lacks steroid 5α-reductase activity in vitro
Chávez, Ramos, García-Becerra et al
Steroids (2014)
Cited by 33
AI Summary: OriGene’s PCMV6-XL4 plasmid containing the human SRD5A2 cDNA (clone SC119922) was used in transfection experiments to assess and compare steroid 5α-reductase activity in cultured cells.
Snippet: 2.6. Steroid 5α-reductase activity in cultured cells. Enzyme activity measurements after transient transfection with pcDNA3.1/hSrd5a3, pcDNA3.1/hSrd5a1, PCMV6-XL4 (containing the human SRD5A2 cDNA;clone SC119922, OriGene Tech. ... Cite SaveSaving
46,XY disorder of sexual development resulting from a novel monoallelic mutation (p.Ser31Phe) in the steroid 5α-reductase type-2 (SRD5A2) gene
Chávez, Ramos, Gómez et al
Mol Genet Genomic Med (2014) 2 (4), 292-6 DOI: 10.1002/mgg3.76
Cited by 8
AI Summary: OriGene’s TrueORF cDNA clone for SRD5A2 was used to express both wild-type and mutant SRD5A2 in HEK-293 cells, enabling functional analysis of the novel p.Ser31Phe mutation described in the study.
FigureFigure
46, XY disorder of sexual development resulting from a novel monoallelic mutation (p. Ser31Phe) in the steroid 5α‐reductase type‐2 (SRD5A2) gene
Chávez, Ramos, Gómez
Molecular Genetics & Genomic Medicine (2014)
AI Summary: OriGene's pCMV6-XL4 vector containing full-length human SRD5A2 cDNA (clone #SC119922) was used as a template to generate constructs for functional assays in the study.
Snippet: 2000). For functional assays, the commercial vector pCMV6-XL4 (OriGene Technologies Inc., Rockville, MD), containing the full-length human SRD5A2 cDNA (clone #SC119922) was used as a template to synthesize constructs. ... Cite SaveSaving
Molecular analysis of the SRD5A2 in 46,XY subjects with incomplete virilization: the P212R substitution of the steroid 5alpha-reductase 2 may constitute an ancestral founder mutation in Mexican patients
Vilchis, Ramos, Méndez et al
J Androl (2010) 31 (4), 358-64 DOI: 10.2164/jandrol.109.009407
Cited by 39
AI Summary: OriGene products, specifically the SRD5A2 cDNA clone (Cat# SC117965) and anti-SRD5A2 antibody (Cat# TA501748), were used in this study to analyze the genetic variants and enzymatic activity of the SRD5A2 gene in patients with steroid 5α-reductase 2 deficiency.
Showing 1-6 of 7 papers.
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