C1QB (NM_000491) Human Untagged Clone

SKU SC119857

C1QB (untagged)-Human complement component 1, q subcomponent, B chain (C1QB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: C1QB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119857 sequence for NM_000491 edited (data generated by NextGen Sequencing); ATGATGATGAAGATCCCATGGGGCAGCATCCCAGTACTGATGTTGCTCCTGCTCCTGGGC; CTAATCGATATCTCCCAGGCCCAGCTCAGCTGCACCGGGCCCCCAGCCATCCCTGGCATC; CCGGGTATCCCTGGGACACCTGGCCCCGATGGCCAACCTGGGACCCCAGGGATAAAAGGA; GAGAAAGGGCTTCCAGGGCTGGCTGGAGACCATGGTGAGTTCGGAGAGAAGGGAGACCCA; GGGATTCCTGGGAATCCAGGAAAAGTCGGCCCCAAGGGCCCCATGGGCCCTAAAGGTGGC; CCAGGGGCCCCTGGAGCCCCAGGCCCCAAAGGTGAATCGGGAGACTACAAGGCCACCCAG; AAAATCGCCTTCTCTGCCACAAGAACCATCAACGTCCCCCTGCGCCGGGACCAGACCATC; CGCTTCGACCACGTGATCACCAACATGAACAACAATTATGAGCCCCGCAGTGGCAAGTTC; ACCTGCAAGGTGCCCGGTCTCTACTACTTCACCTACCACGCCAGCTCTCGAGGGAACCTG; TGCGTGAACCTCATGCGTGGCCGGGAGCGTGCACAGAAGGTGGTCACCTTCTGTGACTAT; GCCTACAACACCTTCCAGGTCACCACCGGTGGCATGGTCCTCAAGCTGGAGCAGGGGGAG; AACGTCTTCCTGCAGGCCACCGACAAGAACTCACTACTGGGCATGGAGGGTGCCAACAGC; ATCTTTTCCGGGTTCCTGCTCTTTCCAGATATGGAGGCCTGA; ; Clone variation with respect to NM_000491.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000491 unedited; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCACTGCTGCTCAGCCCAG; TGGCCTCACAGGACACCAGCTTCCCAGGAGGCGTCTGACACAGTATGATGATGAAGATCC; CATGGGGCAGCATCCCAGTACTGATGTTGCTCCTGCTCCTGGGCCTAATCGATATCTCCC; AGGCCCAGCTCAGCTGCACCGGGCCCCCAGCCATCCCTGGCATCCCGGGTATCCCTGGGA; CACCTGGCCCCGATGGCCAACCTGGGACCCCAGGGATAAAAGGAGAGAAAGGGCTTCCAG; GGCTGGCTGGAGACCATGGTGAGTTCGGAGAGAAGGGAGACCCAGGGATTCCTGGGAATC; CAGGAAAAGTCGGCCCCAAGGGCCCCATGGGCCCTAAAGGTGGCCCAGGGGCCCCTGGAG; CCCCAGGCCCCAAAGGTGAATCGGGAGACTACAAGGCCACCCAGAAAATCGCCTTCTCTG; CCACAAGAACCATCAACGTCCCCCTGCGCCGGGACCAGACCATCCGCTTCGACCACGTGA; TCACCAACATGAACAACAATTATGAGCCCCGCAGTGGCAAGTTCACCTGCAAGGTGCCCG; GTCTCTACTACTTCACCTACCACGCCAGCTCTCGAGGGAACCTGTGCGTGAACCTCATGC; GTGGCCGGGAGCGTGCACAGAAGGTGGTCACCTTCTGTGACTATGCCTACNACACCTTTC; AGGTCACCACCCGTTGGCATGGTCCCTCAAGCTGGAGCAAGGGGGAGAACGTCTTCCTGC; AAGCCACCGACAAGAACTCACTACTGGGCATGGAGGGTGCCAACAGCATCTTTTTCGGGT; TCCTGCTCTTTCCAGATTTGGAGGCCTGACTCTGGGGCTGTTTCAATTCCCCCGGTCTCC; CT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000491 unedited; TCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCCTGAAGAGTTT; ATTTACTCATTCACTCAGCAGCATTCACCAAGCCCTACTGTGTGCATTGGCTGGGCAAGG; GGTGGTGTTGGGGGTAAAATGAACGTTGCTGGCAAGGGGAACCCGGGTGGATGTGAAACA; ACCCACAGGTCAAGCCTCCATATTTGGAAAGAACAAGAACCCGGAAAAAATGCTGTTGGC; ACCCTCCATGCCCAATAGTGAGTTCTTGTCGGTGGCCTGCAAGAAAACCTTCTTCCCCTG; CTCCAGCTTGAGGACCATGCCACCGGTGGTGACCTGGAAAGTGTTGTAAGCATAATCACA; AAAGGTGACCACCTTTTGTGCACGCTTCCGGCCACGCATGAGGTTCACGCACAGGTTCCC; TCGAAAGCTGGCGCGGTACGTGAAATAATAAAGACCGGGCACCTTTGACGTGAACTTGCC; ACTGCGGGGCCCAATAATTGCCGGTCATGTTGGTGAACCCTTGGTCCAAACCGATGGATT; GGTCCCCGCGCAAGGGGACCTTTATTGGCCTTTGTGGCAAAAACCGCATTTTCCGGTTGC; CTTGAATTTACCGATTAACTTTGGGGCCCGGGCCTCAAGGCCCCCTGGTCCCCTTTGGCC; CTTGGCGCCCTGGCGCTACCTCCTTTGGACCTCTAAACACCTCGGTTTCCTTTTTCTACA; TTCCACTGCCCTTACCACCTTCGAATTCCCTTCCCTTTTTTTTTGTTGCCCCCTTTACCC; CATGTTCCCAAATACGCCCCCCCCTCACCCATTTCACCAACCCGTTTTTATTCTCCCCCC; TCCCGTTCCCTTTTTCTCTCCCCCTAAATATTCCCCCCTTCTTACTCTCCCTATTTCCCT; TTCTTCCATACTCCCCCTTACTTCCCATTTTCCTTTTCCCCTCGTCCACTTACTCCTCCC; ACCT;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000491
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000491.2, NP_000482.2
• RefSeq Size: 1072 bp
• RefSeq ORF: 1044 bp
• Locus ID: 713
• UniProt ID: P02746
• Cytogenetics: 1p36.12
• Domains: C1Q, Collagen
• Protein Families: Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• Summary: This gene encodes the B-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. [provided by RefSeq, Dec 2016]