DLST (NM_001933) Human Untagged Clone

SKU SC118954

DLST (untagged)-Human dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (DLST), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DLST
• Synonyms: DLTS; KGD2; PGL7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118954 sequence for NM_001933 edited (data generated by NextGen Sequencing); ATGCTGTCCCGATCCCGCTGTGTGTCTCGGGCGTTCAGCCGCTCGCTCTCCGCCTTCCAG; AAGGGGAACTGCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTCCTTATGCCAGGGACCA; GGTTACCCTAACAGCAGGAAGGTTGTCATTAACAACAGTGTCTTCAGTGTTCGCTTTTTC; AGAACTACAGCTGTATGCAAGGATGACTTGGTTACAGTCAAAACCCCAGCGTTTGCAGAA; TCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCTGTTGGAGACACAGTTGCAGAAGAT; GAAGTGGTTTGTGAGATTGAAACTGACAAGACATCTGTGCAGGTTCCATCACCAGCAAAT; GGCGTGATTGAAGCTCTTTTGGTACCTGATGGGGGAAAAGTCGAAGGAGGCACTCCACTT; TTCACACTCAGGAAAACTGGTGCTGCTCCTGCTAAGGCCAAGCCGGCTGAAGCTCCTGCT; GCTGCAGCCCCAAAAGCAGAACCTACAGCAGCGGCAGTTCCTCCCCCTGCAGCACCCATA; CCCACTCAGATGCCACCGGTGCCCTCGCCCTCACAACCTCCTTCTGGCAAACCTGTGTCT; GCAGTAAAACCCACTGTTGCCCCACCACTAGCTGAGCCAGGAGCTGGCAAAGGTCTGCGT; TCAGAACATCGGGAGAAAATGAACAGGATGCGGCAGCGCATTGCTCAGCGTCTGAAGGAG; GCCCAGAATACATGTGCAATGCTGACAACTTTTAATGAGATTGACATGAGTAACATCCAG; GAGATGAGGGCTCGGCACAAAGAGGCTTTTTTGAAGAAACATAACCTCAAACTAGGCTTC; ATGTCGGCATTTGTGAAGGCCTCAGCCTTTGCCTTGCAGGAACAGCCTGTTGTAAATGCA; GTGATTGACGACACAACCAAAGAGGTGGTGTATAGGGATTATATTGACATCAGTGTTGCA; GTGGCCACCCCACGGGGTCTGGTGGTTCCAGTCATCAGGAATGTGGAAGCTATGAATTTT; GCAGATATTGAACGGACCATCACTGAACTGGGAGAGAAGGCCCGAAAGAATGAACTTGCC; ATTGAAGATATGGATGGTGGTACCTTCACCATTAGCAATGGAGGCGTTTTTGGCTCGCTC; TTTGGAACACCCATTATCAACCCCCCTCAGTCTGCCATCCTGGGGATGCATGGCATCTTT; GACAGGCCAGTGGCTATAGGAGGCAAGGTAGAGGTGCGGCCCATGATGTACGTGGCACTG; ACCTATGATCACCGGCTGATTGATGGCAGAGAGGCTGTGACTTTCCTCCGCAAAATCAAG; GCAGCGGTAGAGGATCCCAGAGTCCTCCTCCTGGATCTTTAG; ; Clone variation with respect to NM_001933.4; 576 g=>a;1098 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001933 unedited; AGTATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGCCCGCCCT; CGGCTCCTCCGCCGTGATGCTGTCCCGATCCCGCTGTGTGTCTCGGGCGTTCAGCCGCTC; GCTCTCCGCCTTCCAGAAGGGGAACTGCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTC; CTTATGCCAGGGACCAGGTTACCCTAACAGCAGGAAGGTTGTCATTAACAACAGTGTCTT; CAGTGTTCGCTTTTTCAGAACTACAGCTGTATGCAAGGATGACTTGGTTACAGTCAAAAC; CCCAGCGTTTGCAGAATCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCTGTTGGAGA; CACAGTTGCAGAAGATGAAGTGGTTTGTGAGATTGAAACTGACAAGACATCTGTGCAGGT; TCCATCACCAGCAAATGGCGTGATTGAAGCTCTTTTGGTACCTGATGGGGGAAAAGTCGA; AGGAGGCACTCCACTTTTCACACTCAGGAAAACTGGTGCTGCTCCTGCTAAGGCCAAGCC; GGCTGAAGCTCCTGCTGCTGCAGCCCCAAAAGCAGAACCTACAGCAGCGGCAGTTCCTCC; CCCTGCAGCACCCATACCCACTCAGATGCCACCGGTGCCCTCGCCCTCACAACCTNCTTC; TGGCAAACCTGTGTCTGCAGTAAAACCCACTGTTGCCCCACCACTAGCTGAGCCAGGAGC; TGGCAAGGTCTGCGTTCAGACATCGGGAGAAAAGAACAGATGCGGCAGCGCATTGCTCAC; CGTCTGAAGGAGGCCCAGATACATGTGCAATGCTGACACCTTTTATGAAGATGACATGAG; TAACATCCAGAAGATGAGGGCTCGGCACAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001933 unedited; GCTATGCACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAAATCT; GCCCTTTAATTTGCATTTAAAATCAGTTTCTTTAAATACTTTAGTTACAAGTTCTGAATA; GTGAAGACAGAGTAGTAGATGCTGCAGGCACTCAGTATTGGCTCCCACCCCTTCCCCCCT; CCCCCACCCCCAGGCCTCACTTCTTGGCTTAAGATACAAAAGGGCACTCTCTACTGGCCC; TGGTGTCAAACGTCAGCAGCCTCAGGCCTGGATGCAGTTGGATACAAAGCCATGAATCCC; AGTGGCTCCAAGGAATTCCAATGATTGTGCCCAGATCCACGGGCAACACACCTTAGAAAT; GACCTCATCCGTTTCACCACTAGGTACTAACACCCTCAGGGTGGTTTCCTACCCCAGTCC; ACGTCCTCCAGATCTGTGTTCTGCACAGGAAGAGACAAGGCCCCGTTATAACGCACGAAG; GAACAAACCCTGCCCCCTCATCCCCGCTGCTTCCCGCTACCTCAACTGCACACCCCAAGG; CACTGCGACGCCACTCTAGACGGCACCCCCCCTCCCCCATTCCCCACCCCCCTTTTGCCC; CCTTTCTGGACATCCTCTCGCCCCCCCGCCCTCATTCGCTCCACACCCCCCTTTCCATCA; ATCGGCCCCACCGAGGCCCACCCTCTCTTTTGGGCTCTCTACGGCCCTTCCCCCCCCTCA; CCGCCACCGCAGTCCCCTTGTTTATTCTTCGTTTCCCCCCCCATTATCCCCCCCTTCCCC; TCCTCCCCACCTTTTCCCTCCCCCCACTCCTTTCCCCCCCGCCCCCATTTTTCTTCCCAC; TATTCCCGCCCCCGTCCCCCCCTATTTTCCTCCCTTTTCCACGCCCCCCCTGCTCTTCAC; TCTCTCCCTTCTTCTTCGTCCCTCCCCCCACGTTTATCCCCCCCACCTCCCTTTTCCCCC; CCTCTCCTTCAGCCCTTCTCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001933
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001933.3, NP_001924.2
• RefSeq Size: 2828 bp
• RefSeq ORF: 1362 bp
• Locus ID: 1743
• UniProt ID: P36957
• Cytogenetics: 14q24.3
• Domains: 2-oxoacid_dh, biotin_lipoyl
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways
• Summary: This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).