MCC (NM_002387) Human Untagged Clone

SKU SC118657

MCC (untagged)-Human mutated in colorectal cancers (MCC), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MCC
• Synonyms: MCC1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002387, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAATTCCGGAGTTGCCATGAAATATGGAAACGACTCCTCGGCCGAGCTGAGTGAGCTCCATTCAGCAG; CCCTGGCATCACTAAAGGGAGATATAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGA; CCTTCTGGAAAAGAAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAGAGAGCATGAGGATGTC; CAGGAGCGAACGACACTTCGCTATGAGGAACGCATCACAGAGCTCCACAGCGTCATTGCGGAGCTCAACA; AGAAGATAGACCGTCTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCAGAACTGCGATCAGAACT; CAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATGGACCAAGACCAGACCTCTGTCTCTATC; CCCGAAAACCAGTCTACCATGGTTACTGCTGACATGGACAACTGCAGTGACCTGAACTCAGAACTGCAGA; GGGTGCTGACAGGGCTGGAGAATGTTGTCTGCGGCAGGAAGAAGAGCAGCTGCAGCCTCTCCGTGGCCGA; GGTGGACAGGCACATTGAGCAGCTCACCACAGCCAGCGAGCACTGTGACCTGGCTATTAAGACAGTCGAG; GAGATTGAGGGGGTGCTTGGCCGGGACCTGTATCCCAACCTGGCTGAAGAGAGGTCTCGGTGGGAGAAGG; AGCTGGCTGGGCTGAGGGAAGAGAATGAGAGCCTGACTGCCATGCTGTGCAGCAAAGAGGAAGAACTGAA; CCGGACTAAGGCCACCATGAATGCCATCCGGGAAGAGCGGGACCGGCTCCGGAGGAGGGTCAGAGAGCTT; CAAACTCGACTACAGAGCGTGCAGGCCACAGGTCCCTCCAGCCCTGGCCGCCTCACTTCCACCAACCGCC; CGATTAACCCCAGCACTGGGGAGCTGAGCACAAGCAGCAGCAGCAATGACATTCCCATCGCCAAGATTGC; TGAGAGGGTGAAGCTATCAAAGACAAGGTCCGAATCGTCATCATCTGATCGGCCAGTCCTGGGCTCAGAA; ATCAGTAGCATAGGGGTATCCAGCAGTGTGGCTGAACACCTGGCCCACTCACTTCAGGACTGCTCCAATA; TCCAAGAGATTTTCCAAACACTCTACTCACACGGATCTGCCATCTCAGAAAGCAAGATTAGAGAGTTTGA; GGTGGAAACAGAACGGCTGAATAGCCGGATTGAGCACCTCAAATCCCAAAATGACCTCCTGACCATAACC; TTGGAGGAATGTAAAAGCAATGCCGAGAGGATGAGCATGCTGGTGGGAAAATACGAATCCAATGCCACAG; CGCTGAGGCTGGCCTTGCAGTACAGCGAGCAGTGCATCGAAGCCTACGAACTCCTCCTGGCGCTGGCAGA; GAGTGAGCAGAGCCTCATCCTGGGGCAGTTCCGAGCGGCGGGCGTGGGGTCCTCCCCTGGAGACCAGTCG; GGGGATGAAAACATCACTCAGATGCTCAAGCGAGCTCATGACTGCCGGAAGACAGCTGAGAACGCTGCCA; AGGCCCTGCTCATGAAGCTGGACGGCAGCTGTGGGGGAGCCTTTGCCGTGGCCGGCTGCAGCGTGCAGCC; CTGGGAGAGCCTTTCCTCCAACAGCCACACCAGCACAACCAGCTCCACAGCCAGTAGTTGCGACACCGAG; TTCACTAAAGAAGACGAGCAGAGGCTGAAGGATTATATCCAGCAGCTCAAGAATGACAGGGCTGCGGTCA; AGCTGACCATGCTGGAGCTGGAAAGCATCCACATCGATCCTCTCAGCTATGACGTCAAGCCTCGGGGAGA; CAGCCAGAGGCTGGATCTGGAAAACGCAGTGCTTATGCAGGAGCTCATGGCCATGAAGGAGGAGATGGCC; GAGTTGAAGGCCCAGCTCTACCTACTGGAGAAAGAGAAGAAGGCCCTGGAGCTGAAGCTGAGCACGCGGG; AGGCCCAGGAGCAGGCCTACCTGGTGCACATTGAGCACCTGAAGTCCGAGGTGGAGGAGCAGAAGGAGCA; GCGGATGCGATCCCTCAGCTCCACCAGCAGCGGCAGCAAAGACAAACCTGGCAAGGAGTGTGCTGATGCT; GCCTCCCCAGCTCTGTCCCTAGCCGAACTCAGGACAACGTGCAGCGAGAATGAGCTGGCTGCGGAGTTCA; CCAACGCCATTCGTCGAGAAAAGAAGTTGAAGGCCAGAGTTCAAGAGCTGGTGAGTGCCTTGGAGAGACT; CACCAAGAGCAGTGAAATCCGACATCAGCAATCTGCAGAGTTCGTGAATGATCTAAAGCGGGCCAACAGC; AACCTGGTGGCTGCCTATGAGAAAGCAAAGAAAAAGCATCAAAACAAACTGAAGAAGTTAGAGTCGCAGA; TGATGGCCATGGTGGAGAGACATGAGACCCAAGTGAGGATGCTCAAGCAAAGAATAGCTCTGCTAGAGGA; GGAGAACTCCAGGCCACACACCAATGAAACTTCGCTTTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002387 unedited; TTCGGCACGAGGCAGCACCCTCAGATCTGCCTAACTGCGTTGAACAGAGACGAGAGAACA; GAGACTTGATGAAATGAGCATCAGCTCCATTCAGCAGCCCTGGCATCACTAAAGGGAGAT; ATAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGACCTTCTGGAAAAG; AAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAGAGAGCATGAGGATGTCCAG; GAGCGAACGACGCTTCGCTATGAGGAACGCATCACAGAGCTCCACAGCGTCATTGCGGAG; CTCAACAAGAAGATAGACCGTCTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCA; GAACTGCGATCAGAACTCAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATG; GACCAAGACCAGACCTCTGTCTCTATCCCCGAAAACCAGTCTACCATGGTTACTGCTGAC; ATGGACAACTGCAGTGACCTGAACTCANAACTGCANAGGGTGCTGACAGGGCTGGAGAAT; GTTGTCTGCGGCAGGAAGAAGAGCAGCTGCAGCCTCTCCGTGGCCGNGGTGGACAGGCAC; ATTGAGCAGCTCACCACAGCCAGCGAGCACTGTGACCTGGCTATTAANACAGTCNANGAG; ATTGAGGGGGTGCTTGGNCGGNACCTGTATTCCCACCTGGCTGANNANAGGTNNTCGTGG; GNAAAAAGAGCTGCTGGNCTGAGGGAANAAANTGAGAGNCTGACTGCCATGCTGTGCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002387 unedited; CAATCCTTGCCCGCGGCCGCAATTTAGATNCGGTTTTTTTTTTTTTTTTTGGGGCATATG; CTTAAATAGTCCAAACATTTTCCTCATGATAAACATGCAACTTTATATGAAAAGTGAAAA; ATTCTAAAAAATCACAGGAATGGCCAGGCACATTGGCTCATGCCTGTAATCCCAGCACTT; TGGGAGGCCGAGGTGGGTGGATCAGTGGTCAGGAGTTCTATATTTTCTGGCCAACATGGT; GAAACCCTGTCTTTACTAAAAATACAAAAAAACTAGCTGGGTATGGTGGCGGGCACCTGT; AATTCCAGCTACTCAGGAGGCAGAGGTTGCAGTGAGCCAAGATCACGCCACTGCACTTCA; GCCTGGTGACAAGAGCAAAACTTCATTTTAAAAAAAAAAAAAAAAAAAATCACAGGAACT; AACTTTGACCAAGTGATTGACAGAAACCAGTGAACATTTTTATAGTGATCAAAGCACCCC; AAAGCTGGTCCTTCTAGACTGATACCAGCTGATCTGTTGTTTCGTTTTGGAGATGGGCAG; CCTCACTGCTGGCCTCTCAGCGGTTGCTTTTAGGATACAGCTGTCTGCCCTCCGAACTGG; TGGCTGGAGTATCCTTCAATGAACAAAAGGCTGTGGAAATTGTATCATGCTTTGAAAAAG; ACCTACCCGTAGCTTATGGACATAAAGACTTTATGTCGAAACCTTCTCCACCTTTTAATT; TGTAGTCAGATATGGCAATGGAACACATTTTTCATAGGGAACTTTGCATGGAATTTCTTG; GTGTTACACACAAAAACAGTAAAGGGGCCCTTTAAGTGTATCCACTGTACACCTGAAAGA; GAAAAGGTAAAACTTACAAACAATATGAACTTTGTTATAGAATGAAAACTCTCCTGGACA; CACCAATTCCCCTGGGACTGCCAACCCTGTAACCATTTCCCCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002387
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002387.1, NP_002378.1
• RefSeq Size: 4181 bp
• RefSeq ORF: 2490 bp
• Locus ID: 4163
• UniProt ID: P23508
• Cytogenetics: 5q22.2
• Protein Families: Druggable Genome
• Summary: This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.