PCNA (NM_002592) Human Untagged Clone

SKU SC118528

PCNA (untagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PCNA
• Synonyms: ATLD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118528 sequence for NM_002592 edited (data generated by NextGen Sequencing); ATGTTCGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAG; GACCTCATCAACGAGGCCTGCTGGGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATG; GACTCGTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTAC; CGCTGCGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAA; TGCGCCGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCG; CTAGTATTTGAAGCACCAAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGAT; TTAGATGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCT; TCTGGTGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATT; TCCTGTGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGAAATGGAAACATT; AAATTGTCACAGACAAGTAATGTCGATAAAGAGGAGGAAGCTGTTACCATAGAGATGAAT; GAACCAGTTCAACTAACTTTTGCACTGAGGTACCTGAACTTCTTTACAAAAGCCACTCCA; CTCTCTTCAACGGTGACACTCAGTATGTCTGCAGATGTACCCCTTGTTGTAGAGTATAAA; ATTGCGGATATGGGACACTTAAAATACTACTTGGCTCCCAAGATCGAGGATGAAGAAGGA; TCTTAG; ; Clone variation with respect to NM_002592.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002592 unedited; NGGGTCAGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCGCT; TGCGGACGCGGCGGCATTAAACGGTTGCAGGACCAGCAGAGTGGTCGTTGTCTTTCTAGG; TCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCTCTGAGGCTCCTGAAGCCGAAACCAG; CTAGACTTTCCTCCTTCCCGCCTGCCTGTAGCGGCGTTGTTGCCACTCCGCCACCATGTT; CGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAGGACCT; CATCAACGAGGCCTGCTGCGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATGGACTC; GTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTACCGCTG; CGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAATGCGC; CGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCGCTAGT; ATTTGAAGCACCCAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGATTTAGA; TGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCTTCTGG; TGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATTTCCTG; CGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGGAATGGAAACATTAAATT; GTCACAGACAAGTAATGTCGATAAAAGANAGGAACCTGTTACCATAGAGATGAATGAACC; AGTNTCACTAACTTTTGCACTGGAGTACCTGAACTTCTTTACAAAGCCACTCCACTCTCT; TCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002592 unedited; CGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATGTTTGAAATTCAAGT; AACTTTATTTAAATTCAAAAACAATTCTTAAAACTGCATTTAGAGTCAAGACCCTTTTGT; ATTATAAAAATCACAAGTATTTCTAAGAGACAAAAATACTTCTAGGTTAACTAGACCAGA; TCTGACTTTGGACTTTATTCTTTAAACAAATTGCAGAGAATAGAGAAAAAAATAGGTTAT; TTACAGAAAACAATATCTACATATGTACTTAGAGGTACAAATTTGGTGACAGAAAAGACT; TCAGTATATGCTGGCATCTTAGAAGCAGTTCTCAAAGAGCTTAGTTTTATTTTCTTGAAT; TTTAAGAATGCCTAAGATCCTTCTTCATCCTCGATCTTGGGAGCCAAGTAGTATTTTAAG; TGTCCCATATCCGCAATTTTATACTCTACAACAAGGGGTACATCTGCAGACATACTGAGT; GTCACCGTTGAAGAGAGTGGAGTGGCTTTTGTAAAGAAGTTCAGGTACCTCAGTGCAAAA; GTTAGTTGAACTGGTTCATTCATCTCTATGGTAACAGCTTCCTCCTCTTTATCGACATTA; CTTGTCTGTGACAATTTAATGTTTCCATTTCCAAGNTCTCCACTTGCAGAAATTTCACTC; CGTCTTTTGCACAGGAAATTACCACAGCATCTCCAATATGCCTGAGATCTCGGCATATAC; GTGCAAATTCACCACGAGGCATCTTTACTACACAGCTGTACCTCTGGTCTGAAATTCCAA; GTTGTTAACATCTAAATCCATCCACTTCATTCATAGCCTGAAAACTTCTCTGGTTTGGGG; CCTTAAAAACTACCGCCAAGTATCCCGGTATCTCCGGCCCTTAGGGATGAAACCTCATTG; CCGGCCTTTTAAATTTTGGCTAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002592
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002592.2, NP_002583.1
• RefSeq Size: 1355 bp
• RefSeq ORF: 786 bp
• Locus ID: 5111
• UniProt ID: P12004
• Cytogenetics: 20p12.3
• Domains: PCNA
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair
• Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.