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PCNA (NM_002592) Human Untagged Clone

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SKU
SC118528
PCNA (untagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PCNA
Synonyms ATLD2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118528 sequence for NM_002592 edited (data generated by NextGen Sequencing)
ATGTTCGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAG
GACCTCATCAACGAGGCCTGCTGGGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATG
GACTCGTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTAC
CGCTGCGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAA
TGCGCCGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCG
CTAGTATTTGAAGCACCAAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGAT
TTAGATGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCT
TCTGGTGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATT
TCCTGTGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGAAATGGAAACATT
AAATTGTCACAGACAAGTAATGTCGATAAAGAGGAGGAAGCTGTTACCATAGAGATGAAT
GAACCAGTTCAACTAACTTTTGCACTGAGGTACCTGAACTTCTTTACAAAAGCCACTCCA
CTCTCTTCAACGGTGACACTCAGTATGTCTGCAGATGTACCCCTTGTTGTAGAGTATAAA
ATTGCGGATATGGGACACTTAAAATACTACTTGGCTCCCAAGATCGAGGATGAAGAAGGA
TCTTAG

Clone variation with respect to NM_002592.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002592 unedited
NGGGTCAGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCGCT
TGCGGACGCGGCGGCATTAAACGGTTGCAGGACCAGCAGAGTGGTCGTTGTCTTTCTAGG
TCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCTCTGAGGCTCCTGAAGCCGAAACCAG
CTAGACTTTCCTCCTTCCCGCCTGCCTGTAGCGGCGTTGTTGCCACTCCGCCACCATGTT
CGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAGGACCT
CATCAACGAGGCCTGCTGCGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATGGACTC
GTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTACCGCTG
CGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAATGCGC
CGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCGCTAGT
ATTTGAAGCACCCAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGATTTAGA
TGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCTTCTGG
TGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATTTCCTG
CGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGGAATGGAAACATTAAATT
GTCACAGACAAGTAATGTCGATAAAAGANAGGAACCTGTTACCATAGAGATGAATGAACC
AGTNTCACTAACTTTTGCACTGGAGTACCTGAACTTCTTTACAAAGCCACTCCACTCTCT
TCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002592 unedited
CGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATGTTTGAAATTCAAGT
AACTTTATTTAAATTCAAAAACAATTCTTAAAACTGCATTTAGAGTCAAGACCCTTTTGT
ATTATAAAAATCACAAGTATTTCTAAGAGACAAAAATACTTCTAGGTTAACTAGACCAGA
TCTGACTTTGGACTTTATTCTTTAAACAAATTGCAGAGAATAGAGAAAAAAATAGGTTAT
TTACAGAAAACAATATCTACATATGTACTTAGAGGTACAAATTTGGTGACAGAAAAGACT
TCAGTATATGCTGGCATCTTAGAAGCAGTTCTCAAAGAGCTTAGTTTTATTTTCTTGAAT
TTTAAGAATGCCTAAGATCCTTCTTCATCCTCGATCTTGGGAGCCAAGTAGTATTTTAAG
TGTCCCATATCCGCAATTTTATACTCTACAACAAGGGGTACATCTGCAGACATACTGAGT
GTCACCGTTGAAGAGAGTGGAGTGGCTTTTGTAAAGAAGTTCAGGTACCTCAGTGCAAAA
GTTAGTTGAACTGGTTCATTCATCTCTATGGTAACAGCTTCCTCCTCTTTATCGACATTA
CTTGTCTGTGACAATTTAATGTTTCCATTTCCAAGNTCTCCACTTGCAGAAATTTCACTC
CGTCTTTTGCACAGGAAATTACCACAGCATCTCCAATATGCCTGAGATCTCGGCATATAC
GTGCAAATTCACCACGAGGCATCTTTACTACACAGCTGTACCTCTGGTCTGAAATTCCAA
GTTGTTAACATCTAAATCCATCCACTTCATTCATAGCCTGAAAACTTCTCTGGTTTGGGG
CCTTAAAAACTACCGCCAAGTATCCCGGTATCTCCGGCCCTTAGGGATGAAACCTCATTG
CCGGCCTTTTAAATTTTGGCTAC
Restriction Sites NotI-NotI |
ACCN NM_002592
Insert Size 1530 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002592.2, NP_002583.1
RefSeq Size 1355 bp
RefSeq ORF 786 bp
Locus ID 5111
UniProt ID P12004
Cytogenetics 20p12.3
Domains PCNA
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair
Summary The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_002592" in other vectors (6)
SKU Description Size Price
RC201741 PCNA (Myc-DDK-tagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 1 10 ug
$300.00
RC201741L1 Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201741L2 Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, mGFP tagged 10 ug
$600.00
RC201741L3 Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201741L4 Lenti ORF clone of Human proliferating cell nuclear antigen (PCNA), transcript variant 1, mGFP tagged 10 ug
$600.00
RG201741 PCNA (tGFP-tagged) - Human proliferating cell nuclear antigen (PCNA), transcript variant 1 10 ug
$489.00 $500.00

file-alt Citations

A Novel Technology for Multiplex Gene Expression Analysis Directly from Whole Blood Samples Stabilized at Ambient Temperature Using an RNA-Stabilizing Buffer
Kim, Abedi, Liu et al
J Mol Diagn (2015) 17 (2), 118-27 DOI: 10.1016/j.jmoldx.2014.11.002
Cited by 11
AI Summary: OriGene’s full-length human cDNA plasmid clones for MRPS18A, MRPS5, PCNA, and FDXR were used to generate in vitro-transcribed RNA, which enabled the authors to assess the sensitivity and limit of detection of their novel multiplex gene expression assay.
Snippet: In Vitro Transcript Titration. Plasmids that contained full-length human cDNA clones for four genes (MRPS18A, MRPS5, PCNA, and FDXR) were obtained from Origene Inc. (Rockville, MD). ... Table 4. Origene Plasmid Clones Used to Generate in Vitro-Transcribed RNA ... Cite SaveSaving
The ADP-ribosyltransferase PARP10/ARTD10 interacts with Proliferating Cell Nuclear Antigen (PCNA) and is required for DNA damage tolerance
Nicolae, Aho, Vlahos et al
J. Biol. Chem. (2014)
AI Summary: In this study, the OriGene PCNA cDNA product was used to express PCNA in cells, enabling the investigation of its interaction with PARP10 and its role in DNA damage tolerance.
Snippet: Plasmids and small interfering RNA (siRNA). The cDNA for PARP10 and PCNA were purchased from PlasmID, and Origene respectively. For transient transfection, cDNA fragments were cloned into pCMV-Myc (Clonetech). ... Cite SaveSaving
Molecular Behavior of CENP-A and PCNA throughout the Cell Cycle in Living Human HT-1080 Cells
Nakagawa, Senda-Murata, Kawakita-Yamaguchi
Mol Biol (2016)
Snippet: Plasmid pmKO-CENP-A was described previously [12]. Plasmid pEGFP-PCNA was constructed by inserting the amplified 0.7 kb fragment of PCNA cDNA from pCMV6-XL5- PCNA (OriGene, Rockville, MD, USA) into the XhoI-BamHI site of pEGFP-C1. ... Cite SaveSaving
Showing 1-3 of 3 papers.
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