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SDHD (NM_003002) Human Untagged Clone

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SKU
SC118276
SDHD (untagged)-Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SDHD
Synonyms CBT1; CII-4; CWS3; cybS; MC2DN3; PGL; PGL1; QPs3; SDH4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_003002 edited
ATGGCGGTTCTCTGGAGGCTGAGTGCCGTTTGCGGTGCCCTAGGAGGCCGAGCTCTGTTG
CTTCGAACTCCAGTGGTCAGACCTGCTCATATCTCAGCATTTCTTCAGGACCGACCTATC
CCAGAATGGTGTGGAGTGCAGCACATACACTTGTCACCGAGCCACCATTCTGGCTCCAAG
GCTGCATCTCTCCACTGGACTAGCGAGAGGGTTGTCAGTGTTTTGCTCCTGGGTCTGCTT
CCGGCTGCTTATTTGAATCCTTGCTCTGCGATGGACTATTCCCTGGCTGCAGCCCTCACT
CTTCATGGTCACTGGGGCCTTGGACAAGTTGTTACTGACTATGTTCATGGGGATGCCTTG
CAGAAAGCTGCCAAGGCAGGGCTTTTGGCACTTTCAGCTTTAACCTTTGCTGGGCTTTGC
TATTTCAACTATCACGATGTGGGCATCTGCAAAGCTGTTGCCATGCTGTGGAAGCTCTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003002 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGGAACGAGATGGCG
GTTCTCTGGAGGCTGAGTGCCGTTTGCGGTGCCCTAGGAGGCCGAGCTCTGTTGCTTCGA
ACTCCAGTGGTCAGACCTGCTCATATCTCAGCATTTCTTCAGGACCGACCTATCCCAGAA
TGGTGTGGAGTGCAGCACATACACTTGTCACCGAGCCACCATTCTGGCTCCAAGGCTGCA
TCTCTCCACTGGACTAGCGAGAGGGTTGTCAGTGTTTTGCTCCTGGGTCTGCTTCCGGCT
GCTTATTTGAATCCTTGCTCTGCGATGGACTATTCCCTGGCTGCAGCCCTCACTCTTCAT
GGTCACTGGGGCCTTGGACAAGTTGTTACTGACTATGTTCATGGGGATGCCTTGCAGAAA
GCTGCCAAGGCAGGGCTTTTGGCACTTTCAGCTTTAACCTTTGCTGGGCTTTGCTATTTC
AACTATCACGATGTGGGCATCTGCAAAGCTGTTGCCATGCTGTGGAAGCTCTGACCTTTT
TGACTTCATACTTTGAAGAATTGATGTATGCCTCTTTGCCTCTGCTTTGTCATGCCATTA
AGCTCACAATAAGGAAGAAATAACAGATAAGTCCATTGGTGGACAGCCTTCTTCTCTTAA
TCACAAGATTATTTTCAGAATTTAATCTTTGAGGANNAGGTTTGAGAGGATTATATCTAA
GTTGTGAGACTGANGTCTATATTCTGGTGAGTTAATGGGGGTGCCTCCCAGCTTCTTATA
GACTCACAGTATACTAACATGAATATCAGCTTTTGCCTTTCAATTATCAATCTCTTAAGA
GATCCCACTTTTTACGATAGNATTGATCAACTTCCTATTGCCTTGGGATAATGACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003002 unedited
GCCGCAATCNATAGTCGAGTTTTTTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTAAAAAAAAAACATTTTATTTAAAAAAATTTTTTTCATAAAATAC
ATTTTCACATTAAAAATTCCCATTGGGCGAAAATAACAATTTATTACTTATAGTTTTATA
TTTGGGGACAGATTGTTTTAAAACATTTTAAATACATTGGGGAATTAAATCTCAATTTAC
AGGGGGGAGTATTTTGATACATCTACGGGGAAACTTGCCCTTAAATGGAACTTTTGTTTA
TTCAAAACCCCTCCAAGCGTTTTTTCCTAGGATTTAAAAATTTATAATTTGAGCTTTCAG
CCTTTCCTAATTTTAAAATTTCCCTACTATATGTAACCATCAGTAGGGGGTCTCTCCCGG
CTACAGAGGGAAGTTTTCCAAAATTAAACCTTGGTTCATTTTCCGCAAAGCTTTTTTTCA
TGAATTAACAGTCTTCCCCTTTGCCCATTATTCCCAAGGGAAATCGGGAAGTTTGATCCT
TTCTAACCGCATTAAAGCGGGTTCTCTTTTAAAAGATTGACAAATTGAAAGGCAAAAGCT
GATCTTTCCTGTTTACTCATCACGGGGAGTTTCATCAAAAGCCGGGCGGCCTCCCCTCTA
CTTCCCAAATATAAAACTTGTTCCCCCCTCAAATATATTCTTCTAAACCCTCGCCACAGA
TACAACCTAAAACACCTTGGACAACAAAAAAGGTTCCCCCCGGGGCTTTTGTTTATTCCC
CATGGGACCTTTGGCTTGCCAACCCGGCCATGGGTTTCTTTCTCTTTCATGTGACCCATT
CGGTAACCCTTCCACCTGGCAACTTTGCCAGCCCACTTGGGTGTGTACCGTAATCTTCAG
GCCACTTTAAGTCCAACCCCGTTTGGATCTTCCAGGTCCCCCGCCAAT
Restriction Sites NotI-NotI |
ACCN NM_003002
Insert Size 1320 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003002.1, NP_002993.1
RefSeq Size 1313 bp
RefSeq ORF 480 bp
Locus ID 6392
UniProt ID O14521
Cytogenetics 11q23.1
Protein Pathways Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Summary This gene encodes a member of complex II of the respiratory chain, which is responsible for the oxidation of succinate. The encoded protein is one of two integral membrane proteins anchoring the complex to the matrix side of the mitochondrial inner membrane. Mutations in this gene are associated with the formation of tumors, including hereditary paraganglioma. Transmission of disease occurs almost exclusively through the paternal allele, suggesting that this locus may be maternally imprinted. There are pseudogenes for this gene on chromosomes 1, 2, 3, 7, and 18. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2013
Transcript Variant: This variant (1) encodes the longest isoform (a).
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Documents
Resources
"NM_003002" in other vectors (6)
SKU Description Size Price
RC203269 SDHD (Myc-DDK-tagged)-Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein 10 ug
$150.00
RC203269L1 Lenti ORF clone of Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC203269L2 Lenti ORF clone of Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RC203269L3 Lenti ORF clone of Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC203269L4 Lenti ORF clone of Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG203269 SDHD (tGFP-tagged) - Human succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), nuclear gene encoding mitochondrial protein 10 ug
$489.00

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