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SFRP1 (NM_003012) Human Untagged Clone

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SKU
SC118246
SFRP1 (untagged)-Human secreted frizzled-related protein 1 (SFRP1)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SFRP1
Synonyms FRP; FRP-1; FRP1; FrzA; SARP2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118246 sequence for NM_003012 edited (data generated by NextGen Sequencing)
ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG
CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG
TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC
ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC
CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG
CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC
TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC
GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC
CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC
CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA
CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT
GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG
AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG
CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG
CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA
ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTGA

Clone variation with respect to NM_003012.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003012 unedited
TATAGGCGGCCGCGAATTCGGCACGAGGGCAGCCCCGACGTCGCGGAGACAGGGCGCAGA
GCCGGCATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCCCTGGGCGTGCTGCTG
GCGCTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTC
CAGTCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTG
GACATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCC
AACCTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCCAGCAGCTGGGTGCC
CCTGCTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCC
CGTCTGCCTGGACCGGCCCATCTACCCGTGTTCGCTGGCTCTGCGAGGCCGTGCGCGACT
CGTGCGAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACA
AGTTCCCGAGGGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCC
AAGCCCCAAGACAAACGGGTGTGTCCTCCCTGTGACACGAGTTGAAATCTGAGGCCATCA
TTGGACATCTCTGTGCAANNCGAGTTTGCNCTGGAGGATGAAATAAAAAGAAGTGAAAAA
GAAATGGCGACAGGAAAATGGTCCCCAGAGAAGAAGCCCTGGGGGTTGGGGGCCATCAAN
AANAAAGACTGAAAAACCTTGGGCTGACTTGAAAAAGGGGGGTGGATTGCCCTGGCACCA
GTGGACAACTTAGCCCCATTTCTCATATGGCCCAAAGGGGGAAACCAGACTTTGTGGCGG
CATCCCACATGGGACAGAAAAAAAGGGGTCAAAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003012 unedited
NAATCTATGNACGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTGATTAAT
TGAGTGACTTTAGTCAAGTGAATATTGATACATGNGCTGAAAAAGCATGAAAATAAAATG
AACACGTACGGGAATTACTATTAACATAAGCGATAACATCAAAACATCTGGTAAAATGCA
GTTAAAAAAACAACACAAATGAAATGGAATGTAAAACATTTTCACAGTATTCAAAGCTTT
TGTAAGAGACTTAGGATCTAAAAGAGGAGTACTACAGGAAGAAACTAGAGGAAACGGGAA
TTTCATCCATGTCCTGTGTATCTGCTGGCAACAGGTCAGAACGGCCAGTATGTTATTCCC
TGCAGGCTGCCTAGGGTGCTCTCCTCAAACAGATCACCTGAGCCTCCTGCATCTATGAAG
TTATGACACAGCAACCAGTTACTCAGAGTCTGATGAGAAAAACAGATTTTAGGTTTGGGA
AATGGGATTACTGTAATTTACACATCCAAATGCAAACTGGAGCTCTGATTGAATTCTACC
CTGNGGAGAACTTGATGCTAACCCACAGGTACCAAGAGCCAAGTGTTACACAGGATATTN
TAAAAATAAAATGTTTTTGGAATCCTCACCTNCCATGCTATCTTCTAAGAAACTACAAAT
ATTCTTCAAAGATTTACCTGAGTTCTGCCAAGGACCTCCCAGGACTCTATCCAGAATGGA
TATTTGTAAAGCTTTACAAATNCCACCTTGGCCTTAGCGAATATTAGGAAATNACAGGGC
AACCTCCTCTCTCGGGAACCATGACCAGGCCATTAGTCTGACATTGGGTTTGTTAAAACC
TTGGGGGAAAAACAAAGCTCGGGATAATGCACTCTTGGCCTCAAGAAAGCCCCTTTTGGG
TTTGAAATGGCGGAGGGACAAAAAAACTTGTATGGGTTAAAACTCAAATAGAACTATTGA
CCTA
Restriction Sites NotI-NotI |
ACCN NM_003012
Insert Size 4300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation A True Clone.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003012.3, NP_003003.3
RefSeq Size 4465 bp
RefSeq ORF 945 bp
Locus ID 6422
UniProt ID Q8N474
Cytogenetics 8p11.21
Domains FRI, NTR
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
Protein Pathways Wnt signaling pathway
Summary This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. provided by RefSeq, Sep 2009
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Documents
Resources
"NM_003012" in other vectors (6)
SKU Description Size Price
RC207328 SFRP1 (Myc-DDK-tagged)-Human secreted frizzled-related protein 1 (SFRP1) 10 ug
$300.00
RC207328L1 Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), Myc-DDK-tagged 10 ug
$600.00
RC207328L2 Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), mGFP tagged 10 ug
$600.00
RC207328L3 Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), Myc-DDK-tagged 10 ug
$600.00
RC207328L4 Lenti ORF clone of Human secreted frizzled-related protein 1 (SFRP1), mGFP tagged 10 ug
$600.00
RG207328 SFRP1 (tGFP-tagged) - Human secreted frizzled-related protein 1 (SFRP1) 10 ug
$489.00 $500.00

file-alt Citations

Secreted-frizzled related protein 1 is a transcriptional repression target of the t(8;21) fusion protein in acute myeloid leukemia
Cheng, Li, Cheng et al
Blood (2011) 118 (25), 6638-48 DOI: 10.1182/blood-2011-05-354712
Cited by 43
AI Summary: OriGene's human SFRP1 cDNA expression plasmid (pCMV-SFRP1) was used in this study to investigate the functional effects of SFRP1 on Wnt signaling and cellular proliferation in acute myeloid leukemia cells.
Snippet: School of Medicine). pCMV-RUNX1-ETOΔ469 and mutant SFRP1 promoter construct were prepared as previously described. 25 Human SFRP1 cDNA expression plasmid (pCMV-SFRP1) was obtained from Origene. T-cell factor
Showing 1-1 of 1 papers.
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