SFRP1 (NM_003012) Human Untagged Clone

SKU SC118246

SFRP1 (untagged)-Human secreted frizzled-related protein 1 (SFRP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SFRP1
• Synonyms: FRP; FRP-1; FRP1; FrzA; SARP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118246 sequence for NM_003012 edited (data generated by NextGen Sequencing); ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG; CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG; TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC; ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC; CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG; CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC; TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC; GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC; CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC; CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA; CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT; GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG; AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG; CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG; CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA; ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTGA; ; Clone variation with respect to NM_003012.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003012 unedited; TATAGGCGGCCGCGAATTCGGCACGAGGGCAGCCCCGACGTCGCGGAGACAGGGCGCAGA; GCCGGCATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCCCTGGGCGTGCTGCTG; GCGCTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTC; CAGTCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTG; GACATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCC; AACCTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCCAGCAGCTGGGTGCC; CCTGCTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCC; CGTCTGCCTGGACCGGCCCATCTACCCGTGTTCGCTGGCTCTGCGAGGCCGTGCGCGACT; CGTGCGAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACA; AGTTCCCGAGGGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCC; AAGCCCCAAGACAAACGGGTGTGTCCTCCCTGTGACACGAGTTGAAATCTGAGGCCATCA; TTGGACATCTCTGTGCAANNCGAGTTTGCNCTGGAGGATGAAATAAAAAGAAGTGAAAAA; GAAATGGCGACAGGAAAATGGTCCCCAGAGAAGAAGCCCTGGGGGTTGGGGGCCATCAAN; AANAAAGACTGAAAAACCTTGGGCTGACTTGAAAAAGGGGGGTGGATTGCCCTGGCACCA; GTGGACAACTTAGCCCCATTTCTCATATGGCCCAAAGGGGGAAACCAGACTTTGTGGCGG; CATCCCACATGGGACAGAAAAAAAGGGGTCAAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003012 unedited; NAATCTATGNACGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTGATTAAT; TGAGTGACTTTAGTCAAGTGAATATTGATACATGNGCTGAAAAAGCATGAAAATAAAATG; AACACGTACGGGAATTACTATTAACATAAGCGATAACATCAAAACATCTGGTAAAATGCA; GTTAAAAAAACAACACAAATGAAATGGAATGTAAAACATTTTCACAGTATTCAAAGCTTT; TGTAAGAGACTTAGGATCTAAAAGAGGAGTACTACAGGAAGAAACTAGAGGAAACGGGAA; TTTCATCCATGTCCTGTGTATCTGCTGGCAACAGGTCAGAACGGCCAGTATGTTATTCCC; TGCAGGCTGCCTAGGGTGCTCTCCTCAAACAGATCACCTGAGCCTCCTGCATCTATGAAG; TTATGACACAGCAACCAGTTACTCAGAGTCTGATGAGAAAAACAGATTTTAGGTTTGGGA; AATGGGATTACTGTAATTTACACATCCAAATGCAAACTGGAGCTCTGATTGAATTCTACC; CTGNGGAGAACTTGATGCTAACCCACAGGTACCAAGAGCCAAGTGTTACACAGGATATTN; TAAAAATAAAATGTTTTTGGAATCCTCACCTNCCATGCTATCTTCTAAGAAACTACAAAT; ATTCTTCAAAGATTTACCTGAGTTCTGCCAAGGACCTCCCAGGACTCTATCCAGAATGGA; TATTTGTAAAGCTTTACAAATNCCACCTTGGCCTTAGCGAATATTAGGAAATNACAGGGC; AACCTCCTCTCTCGGGAACCATGACCAGGCCATTAGTCTGACATTGGGTTTGTTAAAACC; TTGGGGGAAAAACAAAGCTCGGGATAATGCACTCTTGGCCTCAAGAAAGCCCCTTTTGGG; TTTGAAATGGCGGAGGGACAAAAAAACTTGTATGGGTTAAAACTCAAATAGAACTATTGA; CCTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003012
• Insert Size: 4300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A True Clone.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003012.3, NP_003003.3
• RefSeq Size: 4465 bp
• RefSeq ORF: 945 bp
• Locus ID: 6422
• UniProt ID: Q8N474
• Cytogenetics: 8p11.21
• Domains: FRI, NTR
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
• Protein Pathways: Wnt signaling pathway
• Summary: This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]