STAT4 (NM_003151) Human Untagged Clone

SKU SC118193

STAT4 (untagged)-Human signal transducer and activator of transcription 4 (STAT4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: STAT4
• Synonyms: SLEB11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003151, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCTCAGTGGAATCAAGTCCAACAGTTAGAAATCAAGTTTTTGGAGCAGGTGGATCAATTCTATGATG; ACAACTTTCCCATGGAAATTCGGCATCTGTTGGCCCAATGGATTGAAAATCAAGACTGGGAGGCAGCTTC; TAACAATGAAACCATGGCAACGATTCTTCTTCAAAACTTGTTAATACAACTGGATGAACAGTTAGGTCGT; GTTTCCAAAGAGAAAAACCTACTCTTGATACACAATCTAAAAAGAATTAGGAAGGTCCTTCAGGGAAAAT; TTCATGGAAATCCAATGCATGTAGCTGTGGTTATTTCAAACTGTTTAAGGGAAGAGAGGAGAATATTGGC; TGCAGCCAACATGCCTGTCCAGGGGCCTCTAGAGAAATCCTTACAAAGTTCTTCAGTTTCAGAAAGACAG; AGGAATGTGGAGCACAAAGTGGCTGCCATTAAAAACAGTGTGCAGATGACAGAACAAGATACCAAATACT; TAGAAGATCTGCAAGACGAATTTGACTACAGGTATAAAACAATTCAGACAATGGATCAGAGTGACAAGAA; TAGTGCCATGGTGAATCAGGAAGTTTTGACACTGCAGGAAATGCTTAACAGCCTCGATTTCAAGAGAAAG; GAGGCTCTCAGTAAAATGACCCAAATCATCCATGAGACAGACCTGTTAATGAACACCATGCTCATAGAAG; AGCTGCAAGACTGGAAGCGGCGGCAGCAAATCGCCTGCATCGGGGGTCCACTCCACAATGGGCTCGACCA; GCTTCAGAACTGCTTTACACTATTGGCAGAAAGTCTTTTCCAACTGAGAAGGCAATTGGAGAAACTAGAG; GAGCAATCTACCAAAATGACATATGAAGGTGATCCCATTCCAATGCAAAGAACTCACATGCTAGAAAGAG; TCACCTTCTTGATCTACAACCTTTTCAAGAACTCATTTGTGGTTGAGCGACAGCCATGTATGCCAACCCA; CCCTCAGAGGCCGTTGGTACTTAAAACCCTAATTCAGTTCACTGTAAAACTAAGGCTACTAATAAAATTG; CCAGAACTAAACTATCAGGTAAAGGTTAAGGCATCAATTGACAAGAATGTTTCAACTCTAAGCAACCGAA; GATTTGTACTTTGTGGAACTAATGTCAAAGCCATGTCTATTGAAGAATCTTCCAATGGGAGTCTCTCAGT; AGAATTTCGACATTTGCAACCAAAGGAAATGAAGTCCAGTGCTGGAGGTAAAGGAAATGAGGGCTGTCAC; ATGGTGACTGAAGAACTTCATTCCATAACGTTTGAAACACAGATCTGCCTCTATGGCCTGACCATAGATT; TGGAGACCAGCTCATTGCCTGTGGTGATGATTTCCAATGTCAGTCAGTTACCTAATGCTTGGGCATCCAT; CATTTGGTACAACGTGTCAACCAACGATTCCCAGAACTTGGTTTTCTTTAATAATCCTCCACCTGCCACA; TTGAGTCAACTACTGGAGGTGATGAGCTGGCAGTTTTCATCGTACGTTGGTCGTGGTCTTAACTCAGATC; AACTCCATATGCTGGCAGAGAAGCTTACAGTCCAATCTAGCTACAGTGATGGTCACCTCACCTGGGCCAA; GTTCTGCAAGGAACATTTACCTGGTAAATCATTTACCTTTTGGACATGGCTTGAAGCAATATTGGATCTA; ATTAAGAAACACATTCTTCCCCTTTGGATTGATGGGTATGTCATGGGCTTTGTTAGCAAAGAGAAGGAAC; GGCTGTTGCTAAAGGATAAAATGCCTGGCACCTTTTTATTAAGATTCAGTGAAAGCCATCTCGGAGGAAT; AACTTTCACCTGGGTGGACCATTCTGAAAGTGGGGAAGTGAGATTCCACTCTGTAGAACCCTACAATAAA; GGCCGGTTGTCTGCTCTGCCATTCGCTGACATCCTGCGAGACTACAAAGTTATTATGGCTGAAAACATTC; CTGAAAACCCTCTGAAGTACCTATATCCTGACATTCCCAAAGACAAAGCCTTCGGTAAACACTACAGCTC; TCAGCCTTGCGAAGTTTCAAGACCAACAGAAAGGGGTGACAAAGGTTATGTTCCTTCTGTTTTTATCCCC; ATCTCAACAATCCGAAGTGATTCAACAGAGCCACATTCTCCATCAGACCTTCTTCCCATGTCTCCAAGTG; TGTATGCGGTGTTGAGAGAAAACCTGAGTCCCACAACAATTGAAACTGCAATGAAGTCTCCTTATTCTGC; TGAATGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003151 unedited; NGGTTCGTTTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGGGACACT; CACCCTTGGAACCCAGCTGCCATATTGTGAGGAAGCTCAAGCCACAGGGAAGGCCATGTG; TAGGTGTTTGGGCTGATGGTGCCAGCTGAGGACCTGGTCTACTGCCAGTACAACTTATAT; CAAGCATGTGAGTTGGCGAGCTTTTGAAAGAACTCCAGCCACAGTCACTGACCACGCTGC; CTGAGAGACCTAGAGAGAAAACCTAGCTGAGCCAAATCAGGCCCCGGAAACCATGTCTCA; GTGGAATCAAGTCCAACAGTTAGAAATCAAGTTTTTGGAGCAGGTGGATCAATTCTATGA; TGACAACTTTCCCATGGAAATTCGGCATCTGTTGGCCCAATGGATTGAAAATCAAGACTG; GGAGGCAGCTTCTAACAATGAAACCATGGCAACGATTCTTCTTCAAAACTTGTTAATACA; ACTGGATGAACAGTTAGGTCGTGTTTCCAAAGAGAAAAACCTACTCTTGATACACAATCT; AAAAAGAATTAGGAAGGTCCTTCAGGGAAAATTTCATGGAAATCCAATGCATGTAGCTGT; GGTTATTTCAAACTGTTTAAGGGAAGAGAGGAGAATATTGGCTGCAGCCAACATGCCTGT; CCAGNGGCCTCTAGAGAAATCCTTACAAAGTTCTTCAGTTTCAGAAAGACAGAGGAATGT; GGAGCACAAAGTGGCTGCCATTAAAAACAGTGTGCAGATGACAGAACAAGATACCANATA; CTTAGNAAGATCTGCAAGACGAANTTGACTACNAGGTATANACAATTCAGACCATGGATC; AGAGTGACAAGAATAGTGCCATGGTGATCCAAGNAAGTTTTGACACTGCAGNAAATGCTT; AACAGCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003151 unedited; GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTGTTAATATTGTTATTAACAC; TGGTTTCTTAAAGTTGTCTTATCTTGCAAGTTTATCTGAAGCTTTGGTTTCAAGCATTTC; AGTCACAACACTCCCAATTGAGGAGTGCCACGGGAGTGTGAAGAGAGCTTCAGATGTCAA; ACATTTCCTAGAACCTGGTATTTACAAAGCTGAAGAAATAAAATGTGGTTATTGGGCAAA; GAACAGTCTTTAAACTTTTTCATTTGCTTCCTTTCTTGGTGCGTCAGAGTTTATCCTGTC; ATTCAGCAGAATAAGGAGACTTCATTGCAGTTTCAATTGTTGTGGGACTCAGGTTTTCTC; TCAACACCGCATACACACTTGGAGACATGGGAAGAAGGTCTGATGGAGAATGTGGCTCTG; TTGAATCACTTCGGATTGTTGAGATGGGGATAAAAACAGAAGGAACATAACCTTTGTCAC; CCCTTTCTGTTGGTCTTGAAACTTCGCAAGGCTGAGAGCTGTAGTGTTTACCGAAGGCTT; TGTCTTTGGGAATGTCAGGATATAGGTACTTCAGAGGGTTTTCAGGAATGTTTTCAGCCA; TAATAACTTTGTAGTCTCGCAGGATGTCAGCGAATGGCAGAGCAGACAACCGGCCTTTAT; TGTANGGTTCTACAGAGTGGAATCTCACTTCCCCACTTTCAGAATGGTCCACCCCAGTGA; AAAGTATTCCTCCGAGATGGCTTTCACTGAATCTTATAAAAAGGTGCCAGGCATTTTATC; CTTTAGCACAGCCGTTCCTTCTCTTTGCTAACAAAGCCCATGACATACCCATCAATCCAA; AGGGAAGAATGTNGTTNCTAAATAGATCCAATATGCTCCAGCCTGTCCAAAGTAATGATT; ACCAGGTAATGTTCTGCAAACTGGGCCGGTGAGTGACATACTGTACTTGATGACTGAAGC; TTCCTGCACTTGGAGTGACTGAGTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003151
• Insert Size: 3070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003151.2, NP_003142.1
• RefSeq Size: 2761 bp
• RefSeq ORF: 2247 bp
• Locus ID: 6775
• UniProt ID: Q14765
• Cytogenetics: 2q32.2-q32.3
• Domains: SH2, STAT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway
• Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.