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Serum Response Factor (SRF) (NM_003131) Human Untagged Clone

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SKU
SC118177
SRF (untagged)-Human serum response factor (c-fos serum response element-binding transcription factor) (SRF)
  $474.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Serum Response Factor
Synonyms MCM1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_003131, the custom clone sequence may differ by one or more nucleotides


ATGTTACCGACCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAGCCTGAACC
GGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACACGCGGGGCTAACGGGGGCCGGGTCCCCGGGAA
TGGCGCGGGGCTCGGGCCCGGCCGCCTGGAGCGGGAGGCTGCGGCAGCGGCGGCAACCACCCCGGCGCCC
ACCGCGGGGGCCCTCTACAGCGGCAGCGAGGGCGACTCGGAGTCGGGCGAGGAGGAGGAGCTGGGCGCCG
AGCGGCGCGGCCTGAAGCGGAGCCTGAGCGAGATGGAGATCGGTATGGTGGTCGGTGGGCCCGAGGCGTC
GGCAGCGGCCACCGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGGGCCAAGCCGGGTAAGAAGACC
CGGGGCCGCGTGAAGATCAAGATGGAGTTCATCGACAACAAGCTGCGGCGCTACACGACCTTCAGCAAGA
GGAAGACGGGCATCATGAAGAAGGCCTATGAGCTGTCCACGCTGACAGGGACACAGGTGCTGTTGCTGGT
GGCCAGTGAGACAGGCCATGTGTATACCTTTGCCACCCGAAAACTGCAGCCCATGATCACCAGTGAGACC
GGCAAGGCACTGATTCAGACCTGCCTCAACTCGCCAGACTCTCCACCCCGTTCAGACCCCACAACAGACC
AGAGAATGAGTGCCACTGGCTTTGAAGAGACAGATCTCACCTACCAGGTGTCGGAGTCTGACAGCAGTGG
GGAGACCAAGGACACACTGAAGCCGGCGTTCACAGTCACCAACCTGCCGGGTACAACCTCCACCATCCAA
ACAGCACCTAGCACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCCTTTCCCATCACCAACTACCTGG
CACCAGTGTCTGCTAGTGTCAGCCCCAGTGCTGTCAGCAGTGCCAATGGGACTGTGCTGAAGAGTACAGG
CAGCGGCCCTGTCTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGCTTCACCCTCATGCCTGGTGGGGCA
GTGGCCCAGCAGGTCCCAGTGCAGGCCATTCAAGTGCACCAGGCCCCACAGCAAGCGTCTCCCTCCCGTG
ACAGCAGCACAGACCTCACGCAGACCTCCTCCAGCGGGACAGTGACGCTGCCCGCCACCATCATGACGTC
ATCCGTGCCCACAACTGTGGGTGGCCACATGATGTACCCTAGCCCGCATGCGGTGATGTATGCCCCCACC
TCGGGCCTGGGTGATGGCAGCCTCACCGTGCTGAATGCCTTCTCCCAGGCACCATCCACCATGCAGGTGT
CACACAGCCAGGTCCAGGAGCCAGGTGGCGTCCCCCAGGTGTTCCTGACAGCATCATCTGGGACAGTGCA
GATCCCTGTTTCAGCAGTTCAGCTCCACCAGATGGCTGTGATAGGGCAGCAGGCCGGGAGCAGCAGCAAC
CTCACCGAGCTACAGGTGGTGAACCTGGACACCGCCCACAGCACCAAGAGTGAATGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_003131 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCACAGGGGCAGGAAA
GTGAGGGCCCAGGTCGGCCCGGGCGTGCAGGGGCCCCGGGTTCGCAGCGGCGGCCGCGGC
AGCGATAGCGGCACTAGCAGCAGCGGGAGTGCCGGGTTGAGCCGGGAAGCCGATGGCGGC
GGCTGCGGCGGCTCCGATTCCTCGCTGACTGCCCGTCCGCCCTCCTGCATCGAGCGCCAT
GTTACCGACCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAG
CCTGAACCGGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACACGCGGGGCTAACGG
GGGCCGGGTCCCCGGGAATGGCGCGGGGCTCGGGCCCGGCCGCCTGGAGCGGGAGGCTGC
GGCAGCGGCGGCAACCACCCCGGCGCCCACCGCGGGGGCCCTCTACAGCGGCAGCGAGGG
CGACTCGGAGTCGGGCGAGGAGGAGGAGCTGGGCGCCGAGCGGCGCGGCCTGAAGCGGAG
CCTGAGCGAGATGGAGATCGGTATGGTGGTCGGTGGGCCCGAGGCGTCGGCAGCGGCCAC
CGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGTGCCAATCCGGGTAAGAAGACCCG
GGGCCGCGTGAAGATCAAGATGGAGTTCATCGACACCAGCTGCGGCGCTACACGACCTTC
AGCAAGAAGAAGACGGGCATCATGAAAGAAGCCTATGAGCTGTCCACGCTGACAGGGACA
CAGGTTGCTGTTGCTGGTGGCCAGTGAGACAGCCATGTGTATACCTTTTGCCACCCGAAA
CTGCAGCCCATGATCACCATGAGACCGGCAAGCACTGATTCAAACCTGCCTCATCTGCAG
ACTCTCCACCCGTTAGACCCCACACAGACANAGGATGATCGCCCTGCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003131 unedited
TTATAGCATTTGGTTTTCACTTTTCGTCTCCTCAAATAAAAAGTTCAAAATCAACTTTAA
GTAAAGCAGCTGGGAGGAGAGGGGGGAAGACAAGGGGGAGAAGGGAACGCTGGTGGTTCC
CTCCAACCCAGGAGCCCCTGGAGGTCCAAGGGGAGCGGCAAGGCAGGGCAGGCGGCCCCA
GGAACCTGCCCAGTGCACAGGCCCAGCTACTCAGGAGCTGAAGGGGGAGAGGACAGCAGT
GTGAGGAAGAAGCCCCCATCTCCCCAGCTGGCCCCCTGAGGGGCAGAGCCAGGGCTCTTT
TGACCCAGCCTGTGACAGACACAGGGCTGACCTGGGGTCAGCAAGGAGGCTGGCAAGAGG
GCCCCAACTGCTTCCTCAGAGGAGGACACGGTGCCCAGCCCCACAGAATGCCTGTGTGTG
GGGAGGTGGGGCACGGTCCCATTGGGTAAGCTAATACTCATGGCAAACATCGAGCCAAGC
TGGCGAGGGGGTCCCATGAAGGAGGACAGCCCCTTCAGCAACAGAAATGTGATGTGGGGC
GGGAACAAAGGGAGGCTGATGGACGACCGTTGCGAGCTTGCATGCCCTTTCCTTTGCCTG
ACTCATAGGCCTCACCCTCGGCCGCCCTTCCTCTTCCCCCCTCCTTCTTCCTTTCTCCCT
CCCCCTTCCTCCCCCCCTCACCTCCCCCTTTTTTCTCCCCTCTCTTCTCTCCCAGATATT
TTTTTTCCCCCTCTCCTCCCCCCCTCCTTTCCCCCTTCCCCCCTTTCTTTTTTATTCGTC
ACATTCCCCGCCCCGTTTCCGTCTTTTCCCCCTCCCATTTCCCCCCCCTTCTCTTTACGT
TCCTTCTTTTTTTTCCCTTTCCTCCCTTCCCTCCTTCTTTCTCCCTTTCTTTTTTCCTTT
TTTCCCCCTCCTTCTTCCTCTTCCCCCTCCTCTTCTCTTCCCTATTTTCTCCCCCCCCAT
TCTTTTCTTTCTTCCTCCCCCTCCTTATCTTCCATCCTCGATTCTTTCTCTTT
Restriction Sites NotI-NotI |
ACCN NM_003131
Insert Size 2740 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003131.2, NP_003122.1
RefSeq Size 4343 bp
RefSeq ORF 1527 bp
Locus ID 6722
UniProt ID P11831
Cytogenetics 6p21.1
Domains MADS
Protein Families Druggable Genome, Transcription Factors
Protein Pathways MAPK signaling pathway
Summary This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2014
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_003131" in other vectors (6)
SKU Description Size Price
RC208596 SRF (Myc-DDK-tagged)-Human serum response factor (c-fos serum response element-binding transcription factor) (SRF) 10 ug
$473.00
RC208596L1 Lenti ORF clone of Human serum response factor (c-fos serum response element-binding transcription factor) (SRF), Myc-DDK-tagged 10 ug
$773.00
RC208596L2 Lenti ORF clone of Human serum response factor (c-fos serum response element-binding transcription factor) (SRF), mGFP tagged 10 ug
$773.00
RC208596L3 Lenti ORF clone of Human serum response factor (c-fos serum response element-binding transcription factor) (SRF), Myc-DDK-tagged 10 ug
$773.00
RC208596L4 Lenti ORF clone of Human serum response factor (c-fos serum response element-binding transcription factor) (SRF), mGFP tagged 10 ug
$773.00
RG208596 SRF (tGFP-tagged) - Human serum response factor (c-fos serum response element-binding transcription factor) (SRF) 10 ug
$489.00 $673.00

file-alt Citations

Novel mutations of the SRF gene in Chinese sporadic conotruncal heart defect patients
Mengmeng, Yuejuan, Sun et al
BMC Med. Genet. (2020) 21 (1), 95 DOI: 10.1186/s12881-020-01032-y
Cited by 10
AI Summary: OriGene’s SRF open reading frame (ORF) clone (Catalog No: SC118177) was used to construct plasmids expressing wild-type and mutant SRF proteins, enabling functional studies of novel SRF mutations identified in Chinese conotruncal heart defect patients.
Snippet: … Plasmids construction The SRF open reading frame (ORF) clone was purchased from OriGene Technologies (Catalog No: SC118177). Primers for site-directed mutagenesis (Mut-G274D forward/reverse and Mut-G294C: forward/reverse) were designed online (https://www.genomics.agilent.com/pri;The SRF open reading frame (ORF) clone was purchased from OriGene Technologies (Catalog No: SC118177). …
FigureFigureFigure
Reduction of miR‑132‑3p contributes to gastric cancer proliferation by targeting MUC13
He, Qu, Wei et al
Mol Med Rep (2017)
AI Summary: OriGene products, specifically 10% goat serum and 1% calf serum, were used as blocking agents in immunohistochemistry experiments to reduce nonspecific antibody binding in this study investigating the role of miR‑132‑3p and MUC13 in gastric cancer proliferation.
Snippet: with 10% goat serum in PBS (Origene Technologies, Inc., Rockville, MD, USA) for 30 min at 37˚C, then incubated with the MUC13 primary antibody (1:80;catalog no. ... In control sections, the primary antibody was replaced with 1% calf serum (Origene Technologies, Inc ... All 3 versions Cite SaveSaving
WB IHC
Smooth muscle expression of lipoma preferred partner is mediated by an alternative intronic promoter that is regulated by serum response factor/myocardin
Petit, Lindskog, Larsson et al
Circ Res (2008) 103 (1), 61-9 DOI: 10.1161/CIRCRESAHA.108.177436
AI Summary: OriGene products contributed to this study by providing the LPP cDNA clone (OriGene, Cat# SC119105), which was used for quantitative RT-PCR analysis to assess LPP expression from the alternative promoter in various tissues.
Showing 1-3 of 3 papers.
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