NIPSNAP1 (NM_003634) Human Untagged Clone

SKU SC117825

NIPSNAP1 (untagged)-Human nipsnap homolog 1 (C. elegans) (NIPSNAP1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NIPSNAP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117825 sequence for NM_003634 edited (data generated by NextGen Sequencing); ATGGCTCCGCGGCTGTGCAGCATCTCTGTGACGGCGCGGCGGCTGCTGGGGGGCCCGGGG; CCTCGCGCTGGGGACGTTGCGTCTGCAGCTGCGGCGCGTTTCTATTCCAAGGACAATGAA; GGCAGCTGGTTCCGCTCCCTCTTTGTTCACAAAGTGGATCCCCGGAAGGATGCCCACTCC; ACCCTGCTGTCCAAGAAGGAAACCAGCAACCTCTATAAGATCCAGTTTCACAATGTAAAG; CCTGAATACCTGGATGCCTACAACAGCCTCACGGAGGCTGTGCTGCCCAAGCTTCACCTG; GATGAGGACTACCCATGCTCACTCGTGGGCAACTGGAACACGTGGTATGGGGAGCAGGAC; CAGGCAGTGCACCTGTGGCGATTCTCAGGTGGCTACCCAGCCCTCATGGACTGCATGAAC; AAGCTCAAAAACAATAAGGAGTACCTGGAGTTCCGAAGGGAGCGGAGCCAGATGCTGCTG; TCCAGGAGAAACCAGCTGCTCCTCGAGTTCAGCTTCTGGAATGAGCCACAGCCCAGAATG; GGTCCCAACATCTATGAGCTGAGGACATACAAGCTCAAGCCAGGAACCATGATCGAGTGG; GGGAACAACTGGGCTCGGGCCATCAAGTACCGGCAGGAGAACCAGGAGGCAGTGGGCGGC; TTCTTCTCACAGATAGGAGAGCTCTACGTGGTGCACCATCTCTGGGCCTATAAAGACCTG; CAGTCTCGGGAGGAGACTCGAAACGCTGCCTGGAGGAAGAGAGGCTGGGATGAAAATGTC; TACTATACAGTCCCCCTGGTGCGACACATGGAGTCTAGGATCATGATCCCCTTGAAGATC; TCGCCTCTGCAGTGA; ; Clone variation with respect to NM_003634.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003634 unedited; NGGGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGAACATGGCT; CCGCGGCTGTGCAGCATCTCTGTGACGGCGCGGCGGCTGCTGGGGGGCCCGGGGCCTCGC; GCTGGGGACGTTGCGTCTGCAGCTGCGGCGCGTTTCTATTCCAAGGACAATGAAGGCAGC; TGGTTCCGCTCCCTCTTTGTTCACAAAGTGGATCCCCGGAAGGATGCCCACTCCACCCTG; CTGTCCAAGAAGGAAACCAGCAACCTCTATAAGATCCAGTTTCACAATGTAAAGCCTGAA; TACCTGGATGCCTACAACAGCCTCACGGAGGCTGTGCTGCCCAAGCTTCACCTGGATGAG; GACTACCCATGCTCACTCGTGGGCAACTGGAACACGTGGTATGGGGAGCAGGACCAGGCA; GTGCACCTGTGGCGATTCTCAGGTGGCTACCCAGCCCTCATGGACTGCATGAACAAGCTC; AAAAACAATAAGGAGTACCTGGAGTTCCGAAGGGAGCGGAGCCAGATGCTGCTGTCCAGG; AGAAACCAGCTGCTCCTCGAGTTCAGCTTCTGGAATGAGCCACAGCCCAGAATGGGTCCC; AACATCTATGAGCTGAGGACATACAAGCTCAAGCCAGGAACCATGATCGAGTGGGGGAAC; AACTGGGCTCGGGCCATCAAGTACCGGCAGGAGAACCAGGAGGCAGTGGGCGGCTTCTTC; TCACAGATAGGAGAGCTCTACGTGGTGCACCATCTCTGGGCCTATAAAGACCTGCAGTCT; CGGGAGGAGACTCGAAACGCTGCCTGNAGGAAGAGAGGGCTGGATGAAAATGTCTACTAT; ANCAGTCCCCTGGTGCGACACATGAGTCTANGATCATGATCCNNCTTGAGATCTCGCCTC; TGCAGTGATGCTGCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003634 unedited; NCCTCNTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTAGTAAATA; TTTAATTGTTTCCATCAGCAATTCCAGCACAAGTTTTCCTGGATGGTAGGCAGAATCAAG; CTACCCAAGGGTTCATGATGAGGTATGGGGGTCACTGAGGAGACCCCCAGAGTCACTGAC; CCCTCCCGCCACCTCCACACACCAGGTGGCCCTGCAGAATGAGGGTTGGGCTGATAGAAT; GTCAATTAGGGGAGACAGGATACAGGGTGAGGGAACAGGGTCTAGCTTGTATATTTGCCT; GCAGGAAGGAGGGAGGGCAGGAGAGACTCTGCATAGAAGGACTGGAACTACACATTTAAG; TTTTCAACCCCAATATGCAGGGGGAAACAGCCAAGCCACTCTCCATCTGTCTAGTATTAG; GAACCTCTCTTCAAGTGGTCTTTTGTCATCTCTGTTCTTCTTCCCAATTCTGTATTCCAG; ATTCCAAATTCTACAATTGAAACCCAAGTTCTGAATTCTAAGTTCTACCTTCCGAGTTCC; TGTTACGTGTCTGTCTCCTGCCCCTAAATCTTTCCTGGCTCCCATCCCTTTCCCCAGCCC; CTCTGGGCAGAATGTCTGAACCTCCTCCTGACCGTGGTGACGAGGTTTGTTTGTATTTGT; TTTTTTGTTTTGGCAGTTCCAGGCTTTTCTCCAGCAGGAGGGACTTGGCTCAGACTGTCC; AGNCAGTGGGTGGCCCAGTGGAAACCAGGGAATTCCTGNAGGCCTGGCCTGGCCTNCTCT; CTTCCCACCCCACCTCTAGCCACTGGACATACTACCTTTTTGTGCACTAAGCTAANCAGA; GGATTTCCAAGGGTGATAACTGNGGNACTGGTGGGGCCTTGAGATGAGAATTTNTAGAAA; GATATATGAAGGCCCTANAAGATCACTATCTTTCATTCAGGNAAATCAGAACTTCTTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003634
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003634.1, NP_003625.1
• RefSeq Size: 2233 bp
• RefSeq ORF: 855 bp
• Locus ID: 8508
• UniProt ID: Q9BPW8
• Cytogenetics: 22q12.2
• Summary: This gene encodes a member of the NipSnap family of proteins that may be involved in vesicular transport. A similar protein in mice inhibits the calcium channel TRPV6, and is also localized to the inner mitochondrial membrane where it may play a role in mitochondrial DNA maintenance. A pseudogene of this gene is located on the short arm of chromosome 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.