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EIF2S2 (NM_003908) Human Untagged Clone

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SKU
SC117677
EIF2S2 (untagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EIF2S2
Synonyms eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_003908 edited
GAATTCGGCACGAGGCTTTCGCTGATGCAAGAGCCTAGTGCGGTGGTGGGAGAGGTATCG
GCAGGGGCAGCGCTGCCGCCGGGGCCTGGGGCTGACCCGTCTGACTTCCCGTCCGTGCCG
AGCCCACTCGAGCCGCAGCCATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCA
AGAAGAAAAAGAAGAAGAAGAAGCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAG
AGGAAACCCAGCCTTCAGAAACAAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATT
TGGAAGCTGATGAAGAGGACACTAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGA
ACTTCTTTAATCAAAAGAAAAAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAG
CTGAAGAAGGTGTAAAGGATCTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAG
AGGATGACCTTGACATTATGCTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCC
CAGATGAGGATGAAATACTAGAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAG
ATGATGGTATCTCATTCAGTAATCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACT
ACACATACGAGGAGCTGCTGAATCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATA
TGGTTGCTGGGGAGAAAAGGAAATTTGTCATGAAACCTCCACAAGTCGTCCGAGTAGGAA
CCAAGAAAACTTCTTTTGTCAACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCA
AACATCTCCTTGCATTTTTGTTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATA
ACCAACTTGTAATCAAAGGAAGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGAT
ATATCAAGGAATATGTCACTTGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGG
ACACACGACTCTATTTCCTACAGTGCGAAACTTGTCATTCTAGATGTTCTGTTGCCAGTA
TCAAAACCGGCTTCCAGGCTGTCACGGGCAAGCGAGCACAGCTCCGTGCCAAAGCTAACT
AATTTGCTAATCACTGATTTTGCAAAGCTTGTTGTGGAGATGTGGCTGGACAGGTTTGCC
ATCAGAGTGGATATACCGTTGTATTAAAAACAAGATAAAAAAGCTGCCAAGATTTTTGGC
GAGTGGTTGGTCTGAAGTCCTTGCAAGACGCTGATGCTCAAGCTGTTGACATACTCATTG
CCTACTTTAACACCTGTCAGAGAAACGTGATATGGGGTAAGGAGGTGCTTTTTTAAAATC
GTTCATAGACTTCTGTAAAATGCAAGATAAATTAAAGTTATTATAACAGTGAAAAAAAAA
AAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003908 unedited
GGCTTTAGNATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCTTTCGC
TGATGCAAGAGCCTAGTGCGGTGGTGGGAGAGGTATCGGCAGGGGCAGCGCTGCCGCCGG
GGCCTGGGGCTGACCCGTCTGACTTCCCGTCCGTGCCGAGCCCACTCGAGCCGCAGCCAT
GTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAGAA
GCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAAAC
AAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGACAC
TAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGAACTTCTTTAATCAAAAGAAAAA
GAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAGCTGAAGAAGGTGTAAAGGATCT
TAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCTTGACATTATGCT
TGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTAGA
GAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGTAA
TCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGAGGAGCTGCTGAA
TCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGAAA
TTTGTCATGAAACCTCCACAGTCGTCCGAGTAGGAACCAAGANAACTTCTTTTGTNCACT
NTACAGATATCTGGTAACTATTACATCGTCAGCNCAAACATCTCCTTGCATTNTTGTTGG
CTGAATG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003908 unedited
CCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCACTGTTATAATAACTTTAATTT
ATCTTGCATTTTACAGAAGTCTATGAACGATTTTAAAAAAGCACCTCCTTACCCCATATC
ACGTTTCTCTGACAGGTGTTAAAGTAGGCAATGAGTATGTCAACAGCTTGAGCATCAGCG
TCTTGCAAGGACTTCAGACCAACCACTCGCCAAAAATCTTGGCAGCTTTTTTATCTTGTT
TTTAATACAACGGTATATCCACTCTGATGGCAAACCTGTCCAGCCACATCTCCACAACAA
GCTTTGCAAAATCAGTGATTAGCAAATTAGTTAGCTTTGGCACGGAGCTGTGCTCGCTTG
CCCGTGACAGCCTGGAAGCCGGTTTTGATACTGGCAACAGAACATCTAGAATGACAAGTT
TCGCACTGTAGGAAATAGAGTCGTGTGTCCTTCTGCAGGATTGTGTCCGGTGATCGGCAT
GTGTGACAAGTGACATATTCCTTGATATATCTTCTCAAGACATTTTCTATCTGTTTCTGT
TGGAATCTTCCTTTGATTACAAGTTGGTTATTACCATCTATAGAACCACTTGTACCCAAT
TCAGCCAACAAAAATGCAAGGAGATGTTTGGGCTGACGATGTAATAGTTTACAGATATCT
GTAAAGTTGACAAAAGAAGTTTTCTTGGTTCCTACTCGGACGACTTGTGGAGGTTTCATG
ACAAATTTCCTTTTCTCCCCACAACCATATCTGAATTCTTTTCCCTCATGATGTTGAACA
CTCCGATCAGCAGCCTCTCGTATGTGTAGTCTCTTTTTGAGCCTGCCCAAGCAGGGCCTG
TCTGATTACTGAATGAGAAACCATCATCCTTTTTTGTTGCCTTCATTCTCTAGAGCTNCA
CCTTCTCTAGACTTCAACCTCATCTGGGAACCTAACATTCTTCTTTTCTTCTTTTATTGC
CAGCATATGCAGGNCATCCTCTGGTTAATTGGTCCTAGACTCACTTAT
Restriction Sites NotI-NotI |
ACCN NM_003908
Insert Size 1430 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003908.2, NP_003899.2
RefSeq Size 1439 bp
RefSeq ORF 1002 bp
Locus ID 8894
UniProt ID P20042
Cytogenetics 20q11.22
Domains eIF2B_5
Summary Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2015
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_003908" in other vectors (6)
SKU Description Size Price
RC201563 EIF2S2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2) 10 ug
$300.00
RC201563L1 Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), Myc-DDK-tagged 10 ug
$600.00
RC201563L2 Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mGFP tagged 10 ug
$600.00
RC201563L3 Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), Myc-DDK-tagged 10 ug
$600.00
RC201563L4 Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mGFP tagged 10 ug
$600.00
RG201563 EIF2S2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2) 10 ug
$489.00 $500.00

file-alt Citations

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