EIF2S2 (NM_003908) Human Tagged ORF Clone
SKU
RC201563
EIF2S2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2)
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Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Target Symbol | EIF2S2 |
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Synonyms | eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC201563 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAGAAGCCTTTTA TGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAAACAAAAGAAGTGGAGCCAGA GCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGACACTAGGAAAAAAGATGCTTCTGATGATCTA GATGACTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAG CTGAAGAAGGTGTAAAGGATCTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCT TGACATTATGCTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTA GAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGTAATCAGACAG GCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGATGAGCTGCTGAATCGAGTGTTCAACATCAT GAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGGAAATTTGTCATGAAACCTCCACAAGTCGTC CGAGTAGGAACCAAGAAAACTTCTTTTGTCAACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCA AACATCTCCTTGCATTTTTGTTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGT AATCAAAGGAAGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACT TGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGGACACACGACTCTATTTCCTACAGTGCGAAA CTTGTCATTCTAGATGTTCTGTTGCCAGTATCAAAACCGGCTTCCAGGCTGTCACGGGCAAGCGAGCACA GCTCCGTGCCAAAGCTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201563 protein sequence
Red=Cloning site Green=Tags(s) MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDL DDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEIL EKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVV RVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVT CHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003908 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_003908.5 |
RefSeq Size | 2592 bp |
RefSeq ORF | 1002 bp |
Locus ID | 8894 |
UniProt ID | P20042 |
Cytogenetics | 20q11.22 |
Domains | eIF2B_5 |
MW | 38.4 kDa |
Summary | Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC201563L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), Myc-DDK-tagged | 10 ug |
$600.00
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RC201563L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mGFP tagged | 10 ug |
$600.00
|
|
RC201563L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), Myc-DDK-tagged | 10 ug |
$600.00
|
|
RC201563L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mGFP tagged | 10 ug |
$600.00
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RG201563 | EIF2S2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2) | 10 ug |
$489.00
MSRP
$500.00
MSRP
$500.00
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SC117677 | EIF2S2 (untagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2) | 10 ug |
$457.00
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.