EEF1E1 (NM_004280) Human Untagged Clone

SKU SC117477

EEF1E1 (untagged)-Human eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EEF1E1
• Synonyms: AIMP3; P18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004280 edited; ATGGCGGCGGCCGCAGAGTTGTCGCTACTGGAGAAGTCCCTGGGACTGAGTAAGGGGAAT; AAATACAGTGCTCAGGGCGAGCGACAGATTCCAGTTCTTCAGACAAACAATGGTCCAAGT; CTAACAGGATTGACTACTATAGCAGCTCATCTAGTCAAGCAAGCCAACAAAGAATATTTG; CTGGGGAGTACTGCAGAAGAAAAAGCAATCGTTCAGCAGTGGTTAGAATACAGGGTCACT; CAAGTAGATGGGCACTCCAGTAAAAATGACATCCACACACTGTTGAAGGATCTTAATTCA; TATCTTGAAGATAAAGTCTACCTTACAGGGTATAACTTTACATTAGCAGATATACTATTG; TACTATGGACTTCATCGCTTTATAGTTGACCTGACAGTTCAAGAAAAGGAGAAATATCTT; AATGTGTCTCGCTGGTTTTGTCACATTCAGCATTATCCAGGCATCAGGCAACATCTGTCT; AGTGTTGTCTTCATCAAGAACAGACTATATACTAATTCCCACTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004280 unedited; GGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGATGGCGGCGG; CCGCAGAGTTGTCGCTACTGGAGAAGTCCCTGGGACTGAGTAAGGGGAATAAATACAGTG; CTCAGGGCGAGCGACAGATTCCAGTTCTTCAGACAAACAATGGTCCAAGTCTAACAGGAT; TGACTACTATAGCAGCTCATCTAGTCAAGCAAGCCAACAAAGAATATTTGCTGGGGAGTA; CTGCAGAAGAAAAAGCAATCGTTCAGCAGTGGTTAGAATACAGGGTCACTCAAGTAGATG; GGCACTCCAGTAAAAATGACATCCACACACTGTTGAAGGATCTTAATTCATATCTTGAAG; ATAAAGTCTACCTTACAGGGTATAACTTTACATTAGCAGATATACTATTGTACTATGGAC; TTCATCGCTTTATAGTTGACCTGACAGTTCAAGAAAAGGAGAAATATCTTAATGTGTCTC; GCTGGTTTTGTCACATTCAGCATTATCCAGGCATCAGGCAACATCTGTCTAGTGTTGTCT; TCATCAAGAACAGACTATATACTAATTCCCACTAGAAGCTGTCCATGCCATACAGAAGAT; CTATTAAAAATGTTTTAAATGGAAAATGTACTCTAGACCACAGGACTAATGTAAATTAAT; ATACAGTCATTCATTATTTGTTGAAGTTGATAGAATTTTTGAAGTGTAAACTTGTGTCTG; AATGTTTTATTTGTTCTTTAGCTGAAGTTTTGCAATTTTTATGTCAAAATTCAATTGCTA; TTAAACCAGTTGAGATCCAGTTANTAATTAACCTTGTTTTTAGTAGATGACATTNNATTT; CATAAAGNTGCAAATCGGGCTTAATCTAAAATGGNGGTCATTNCATGGNNTGACATATTG; GGCTATTATAACCTCTCTTTCTATTCTAANATCATTTCCCCTATGAATNTAGGGGGNNAT; TTGGTAGACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004280 unedited; TATCTTGNACCGCGCCGCTTTCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAACCACATT; TACTAGCTCACATAAATATTTTAAAACAAATCCATCTGTCTTCCCTTTTGGCTTCCTTGG; CACAATTTATCAGTTCTTAACAAACTACCATAAATATCCATAAGGGGAAAATGAATTTTA; GAATATGAAAGAGAGGTTAATAAATAGCCAAATATGTCAACCATTGAAATGACCACCAAT; TTTAAGATTAAGCCCGATTTGCAACTTTTATTGAAATAAATGTCATCTACTAAAAACAAG; GTTAATTTATAACTGGATCTCAACTTGTTTAATAGCAATTGAATTTTGACATAAAAATTG; CAAAACTTCAGCTAAAGAACAAATAAAACATTCAGACACAAGTTTACACTTCAAAAATTC; TATCAACTTCAACAAATAATGAATGACTGTATATTAATTTACATTAGTCCTGTGGTCTAG; AGTACATTTTCCATTTAAAACATTTTTAATAGATCTTCTGTATGGCATGGACAGCTTCTA; GTGGGAATTAGTATATAGTCTGTTCTTGATGAAGACAACACTAGACAGATGTTGCCTGAT; GCCTGGATATGCTGAATGTGACANANCCAGCGAGACACATTAAGATATTTCTCCTTTTTC; TGAACTGTCAGGTCAACTATAAAGCGATGAAGTCCATAGTACAATAGTATATCTGCTTAT; GTAAAGTTATACCCTGTTAGGTAGACTTTNNATCTCAGATATGAATTAAGATCCNTNCAC; AGTGTGTGGATGTCATTTTTACTGGNAGTGCCCATCTACTTGAGTGACCCTGGTTTCTAC; ACCTGCTGAACGATTGCTTTTTCTCTGCAGTACTCCCCAGCAAAAATTCTTTGNTGGCTT; GCTTGACTAAAAGAGCTGCTTTAGTAATCAATCCTGTTAAACTTGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004280
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004280.2, NP_004271.1
• RefSeq Size: 1067 bp
• RefSeq ORF: 525 bp
• Locus ID: 9521
• UniProt ID: O43324
• Cytogenetics: 6p24.3
• Protein Families: Druggable Genome
• Summary: This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).