CAPZB (NM_004930) Human Untagged Clone

SKU SC117036

CAPZB (untagged)-Human capping protein (actin filament) muscle Z-line, beta (CAPZB), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CAPZB
• Synonyms: CAPB; CAPPB; CAPZ
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117036 sequence for NM_004930 edited (data generated by NextGen Sequencing); ATGAGTGATCAGCAGCTGGACTGTGCCTTGGACCTAATGAGGCGCCTGCCTCCCCAGCAA; ATCGAGAAAAACCTCAGCGACCTGATCGACCTGGTCCCCAGTCTATGTGAGGATCTCCTG; TCTTCTGTTGACCAGCCACTGAAAATTGCCAGAGACAAGGTGGTGGGAAAGGATTACCTT; TTGTGTGACTACAACAGAGATGGGGACTCCTATAGGTCACCATGGAGTAACAAGTATGAC; CCTCCCTTGGAGGATGGGGCCATGCCGTCAGCTCGGCTGAGAAAGCTGGAGGTGGAAGCC; AACAATGCCTTTGACCAGTATCGAGACCTGTATTTTGAAGGTGGCGTCTCATCTGTCTAC; CTCTGGGATCTGGATCATGGCTTTGCTGGAGTGATCCTCATAAAGAAGGCTGGAGATGGA; TCAAAGAAGATCAAAGGCTGCTGGGATTCCATCCACGTGGTAGAAGTGCAGGAGAAATCC; AGCGGTCGCACCGCCCATTACAAGTTGACCTCCACGGTGATGCTGTGGCTGCAGACCAAC; AAATCTGGCTCTGGCACCATGAACCTCGGAGGCAGCCTTACCAGACAGATGGAGAAGGAT; GAAACTGTGAGTGACTGCTCCCCACACATAGCCAACATCGGGCGCCTGGTAGAGGACATG; GAAAATAAAATCAGAAGTACGCTGAACGAGATCTACTTTGGAAAAACAAAGGATATCGTC; AATGGGCTGAGGTCTGTGCAGACTTTTGCAGACAAATCAAAACAAGAAGCTCTGAAGAAT; GACCTGGTGGAGGCTTTGAAGAGAAAGCAGCAATGCTAA; ; Clone variation with respect to NM_004930.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004930 unedited; CGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCGGGACCG; GGACCGCCGCCGCCGCCGCCACCATGAGTGATCAGCAGCTGGACTGTGCCTTGGACCTAA; TGAGGCGCCTGCCTCCCCAGCAAATCGAGAAAAACCTCAGCGACCTGATCGACCTGGTCC; CCAGTCTATGTGAGGATCTCCTGTCTTCTGTTGACCAGCCACTGAAAATTGCCAGAGACA; AGGTGGTGGGAAAGGATTACCTTTTGTGTGACTACAACAGAGATGGGGACTCCTATAGGT; CACCATGGAGTAACAAGTATGACCCTCCCTTGGAGGATGGGGCCATGCCGTCAGCTCGGC; TGAGAAAGCTGGAGGTGGAAGCCAACAATGCCTTTGACCAGTATCGAGACCTGTATTTTG; AAGGTGGCGTCTCATCTGTCTACCTCTGGGATCTGGATCATGGCTTTGCTGGAGTGATCC; TCATAAAGAAGGCTGGAGATGGATCAAAGAAGATCAAAGGCTGCTGGGATTCCATCCACG; TGGTAGAAGTGCAGGAGAAATCCAGCGGTCGCACCGCCCATTACAAGTTGACCTCCACGG; TGATGCTGTGGCTGCAGACCAACAAATCTGGCTCTGGCACCATGAACCTCGGAGGCAGCC; TTACCAGACAGATGGAGAAGGATGAAACTGTGAGTGACTGCTCCCCACACATAGCCAACA; TCGGGCGCCTGGTAGAGGACATGGNAAATAAAATCAGAAGTACGCTGAACGAGATCTACT; TTGGAAAAACANAGGATATCGTCATGGGCTGAGGTCTGTGCAGACTTTTGCANGACAATC; AAAACAGNAGCTCTGAAGATGACCTGGTGGAGGCTTTGAAGAGAAAGCANCATGCTAAAC; CTCTGNTTCATGCTAACCAGACACGCCGTGCACTCGTAGATTCCCTTCTAAAACTCGTTT; CTGCTCCTTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004930 unedited; NCACAGGGATCCTACCCCAATCCAGGATCNGTTTAAAATCTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTAAACCGCCCCCCAATTTC; TTTTTATGGATTTCTTTTTTTTTTTTTTTTAAAGTTGGGGGGCCCAAGTTTAAAAAGGAA; ATTTTTTTTTTAACCTTTCCCAAAAAACAAATTTATACAAAACCCCCCTTTTGCCTTCCA; TAAAAATTGACAAAAAACCCCCTGGCCGGAAAAACGGAACGGTGGGGGGAACCCCCCCCA; GCCCCCACCCCGGGGCCTCCGGGGGACGGGAAAATTTGCCCAAGACGGCCCCGAGGGCCC; CCTTTGGTCCCAAAGGTGTCCCCTGGCCCCACAACCCCCAACCCCAAGAAATTCCCAAAC; ACCCCCGGAAAAAAATTTCCAGTTTTTTTTTTTTTTTTCACCCCCCCCAGTTAGTTCAAA; AAATTTTTTTGGTTTACATTTTTTACCCCAATGTCCCAAAAAGGGGGGAGGGAAAGGAGG; CTGGCAAACAGGGGATTTTATCCCCATAAATGGGGGGACAGGGAGAAAAACGGGGGGCCC; GGGTGAACAAAAACCACCCGGTCTTTTTGGAAATGGGGAAAAAACTGAAAGCCAGGGGGG; GCAAAAACAGGCTCGGACCCCGAGGAGGGGGGCTATCGGCTTTATTCTCAAGGAAAGATG; GCCCTGTGCCGTCAATGAAGCACCTTTTATGTGACCCGCCGGGGAGGGAAAGGACCAGGG; GAGGGGACCCCAAAACCAGTTTTTTAAAAAAGAATCTTAACCAGGCCCCGCGGTTGCTTG; GTTAACAAAAACCAAAGGTTTAACATTGGTTGTTTCTTTTTGAAGCCTCCCCCCAGGTCT; TTTTTAAAAATCTTTTGTTTGGATTTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004930
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004930.1, NP_004921.1
• RefSeq Size: 1077 bp
• RefSeq ORF: 819 bp
• Locus ID: 832
• UniProt ID: P47756
• Cytogenetics: 1p36.13
• Domains: F_actin_cap_B
• Summary: This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. A pseudogene of this gene is located on the long arm of chromosome 2. Multiple alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, Aug 2013]; Transcript Variant: This variant (1) encodes isoform 1.