RND3 (NM_005168) Human Untagged Clone

SKU SC116889

RND3 (untagged)-Human Rho family GTPase 3 (RND3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RND3
• Synonyms: ARHE; memB; Rho8; RhoE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116889 sequence for NM_005168 edited (data generated by NextGen Sequencing); ATGAAGGAGAGAAGAGCCAGCCAGAAATTATCCAGCAAATCTATCATGGATCCTAATCAG; AACGTGAAATGCAAGATAGTTGTGGTGGGAGACAGTCAGTGTGGAAAAACTGCGCTGCTC; CATGTCTTCGCCAAGGACTGCTTCCCCGAGAATTACGTTCCTACAGTGTTTGAGAATTAC; ACGGCCAGTTTTGAAATCGACACACAAAGAATAGAGTTGAGCCTGTGGGACACTTCGGGT; TCTCCTTACTATGACAATGTCCGCCCCCTCTCTTACCCTGATTCGGATGCTGTGCTGATT; TGCTTTGACATCAGTAGACCAGAGACCCTGGACAGTGTCCTCAAAAAGTGGAAAGGTGAA; ATCCAGGAATTTTGTCCAAATACCAAAATGCTCTTGGTCGGCTGCAAGTCTGATCTGCGG; ACAGATGTTAGTACATTAGTAGAGCTCTCCAATCACAGGCAGACGCCAGTGTCCTATGAC; CAGGGGGCAAATATGGCCAAACAGATTGGAGCAGCTACTTATATCGAATGCTCAGCTTTA; CAGTCGGAAAATAGCGTCAGAGACATTTTTCACGTTGCCACCTTGGCATGTGTAAATAAG; ACAAATAAAAACGTTAAGCGGAACAAATCACAGAGAGCCACAAAGCGGATTTCACACATG; CCTAGCAGACCAGAACTCTCGGCAGTTGCTACGGACTTACGAAAGGACAAAGCGAAGAGC; TGCACTGTGATGTGA; ; Clone variation with respect to NM_005168.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005168 unedited; GGGTTCAGAATTTGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGG; GCCGGTGAGGTAGTTCAGGCTTAAAACTTGTTGGAGGGGAGTAACCAGCCTGCTCCTCTC; GCTCTCCTCCTCGTCTGCGCCGCGTTTCAGAGGTTGCCCATCAGCCTTGTGATTTATTTT; TATATCTGCTTTTTATAAAGAGAGAAATATATATATATATATATATATATTTTTTTTCTT; CTTAAGAGAAAATTCCTGTTCCAAGAGAAAATAAGGCAACATCAATGAAGGAGAGAAGAG; CCAGCCAGAAATTATCCAGCAAATCTATCATGGATCCTAATCAGAACGTGAAATGCAAGA; TAGTTGTGGTGGGAGACAGTCAGTGTGGAAAAACTGCGCTGCTCCATGTCTTCGCCAAGG; ACTGCTTCCCCGAGAATTACGTTCCTACAGTGTTTGAGAATTACACGGCCAGTTTTGAAA; TCGACACACAAAGAATAGAGTTGAGCCTGTGGGACACTTCGGGTTCTCCTTACTATGACA; ATGTCCGCCCCCTCTCTTACCCTGATTCGGATGCTGTGCTGATTTGCTTTGACATCAGTA; GACCAGAGACCCTGGACAGTGTCCTCAAAAAGTGGAAAGGTGAAATCCAGGAATTTTGTC; CAAATACCAAAATGCTCTTGGTCGGCTGCAAGTCTGATCTGCGGACAGATGTTAGTACAT; TAGTAGAGCTCTCCAATCACAGGCAGACGCCAGTGTCCTATGACCAGNGGGCANATATGG; CCAAACAGATTGGAGCAGCTACTTATATCGAATGCTCAGCTNTACAGTCGGAANATAGCG; TCAGAGACATTTTTCACGTTGCCACCTTGCCATGTGTAAATAAGACAAATAAAACGTTAA; GCGGNACAAATCACAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005168 unedited; TTACTCTTTTATTTTGAGNAAAAAAAAAAGTTGTTTTAATAAATACTGCGGAAACATTGA; CCAAACAAACATACACAGTGACTTCAACAGTTTAAAAAATGCAACAAAAGTTCTGCTTTA; TAACAGTTATAACTTATTTTCTTTTTACAATATTTAAATACAGAAAGCACTCGCCAGCTA; TTTTGTAATACTGCCCAAAGCATAATGCTGCATCAATCAAAGCATATTATGTTGGTAAAA; TGTCTGTATTTTATGGGAAATGACTGGAATCGAATTTTCTTGCAGAGACAAGACAAAGTG; CTTGTAAGACATAGAACTGAGTGTTGTAATTACCTTAATCTCTCCTGCCACGATTTAAGG; GGAGCACAGCTTCAACACTTCAAAAACGAAGCACTATCATGGGAAAGAAAGCTCGTTTTC; AACAAGCGCCAACAATAAACAGGTCAGATCTGGTTTCTTCTCTGAAAGAATCTCTAAAAG; GAACTGGTCAAAAATATCTGTAAACTATTTTAAATGCTTATTATTTTACGTAACAACAGA; GACCAAGTGCTGAACACAGACCTTGGCTGCGTCTGTTCAGTGAGGTTTTAGCAATGACAC; TCTAGAAATGCCCTAGTGTTATGTCAGGACTGAAAGTTTATCATATAATCTTNCACTAGG; GATTCTCTAAAGCTTGGGTAAGTGGCATCTTCTCTCATCAATGTTCATTTAATGAAAAGT; GAAATAAACCACCAGATTATCAGATGCTGTCAGTTTTGGAGCTTCAACCACAAATATAGT; CCTTTTGCTTTGTNATTTTGGTAACTAANTTCAGACAGCTTTACTCACAAACTGGGCTTN; CTGCTACTATTCTTTCCTTCACTCATTACCTTTTCTGGGGAAAACAGAACTACCCTTTTT; CTCTTCTTGGGACATTTCTTGACATAAACACAGCAACCTTTTTTGATTTGGCTTTNTTTA; ACCGTGGCAAAATAACACACACTTTAAACAGCGCCAAAATTTCCTTGGGCATTCACACTT; TTTAGCAATCCATGGAACACAACTCGACGAATAAACGCTATTNTTCCTTTGAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005168
• Insert Size: 2950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005168.3, NP_005159.1
• RefSeq Size: 2683 bp
• RefSeq ORF: 735 bp
• Locus ID: 390
• UniProt ID: P61587
• Cytogenetics: 2q23.3
• Domains: RAB, ras, RAS, RHO
• Summary: This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.