TDO2 (NM_005651) Human Untagged Clone

SKU SC116613

TDO2 (untagged)-Human tryptophan 2,3-dioxygenase (TDO2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TDO2
• Synonyms: HYPTRP; TDO; TO; TPH2; TRPO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116613 sequence for NM_005651 edited (data generated by NextGen Sequencing); ATGAGTGGGTGCCCATTTTTAGGAAACAACTTTGGATATACTTTTAAAAAACTCCCCGTA; GAAGGCAGCGAAGAAGACAAATCACAAACTGGTGTGAATAGAGCCAGCAAAGGAGGTCTT; ATCTATGGGAACTACCTGCATTTGGAAAAAGTTTTGAATGCACAAGAACTGCAAAGTGAA; ACAAAAGGAAATAAAATCCATGATGAACATCTTTTTATCATAACTCATCAAGCTTATGAA; CTCTGGTTTAAGCAAATCCTCTGGGAGTTGGATTCTGTTCGAGAGATCTTTCAGAATGGC; CATGTCAGAGATGAAAGGAACATGCTTAAGGTTGTTTCTCGGATGCACCGAGTGTCAGTG; ATCCTGAAACTGCTGGTGCAGCAGTTTTCCATTCTGGAGACGATGACAGCCTTGGACTTC; AATGACTTCAGAGAGTACTTATCTCCAGCATCAGGCTTCCAGAGTTTGCAATTCCGACTA; TTAGAAAACAAGATAGGTGTTCTTCAGAACATGAGAGTCCCTTATAACAGAAGACATTAT; CGTGATAACTTCAAAGGAGAAGAAAATGAACTGCTACTTAAATCTGAGCAGGAAAAGACA; CTTCTGGAATTAGTGGAGGCATGGCTGGAAAGAACTCCAGGTTTAGAGCCACATGGATTT; AACTTCTGGGGAAAGCTTGAAAAAAATATCACCAGAGGCCTGGAAGAGGAATTCATAAGG; ATTCAGGCTAAAGAAGAGTCTGAAGAAAAAGAGGAACAGGTGGCTGAATTTCAGAAGCAA; AAAGAGGTGCTACTGTCCTTATTTGATGAGAAACGTCATGAACATCTCCTTAGTAAAGGT; GAAAGACGGCTGTCATACAGAGCACTTCAGGGAGCATTGATGATATATTTTTACAGGGAA; GAGCCTAGGTTCCAGGTGCCTTTTCAGTTGCTGACTTCTCTTATGGACATAGATTCACTG; ATGACCAAATGGAGATATAACCATGTGTGCATGGTGCACAGAATGCTGGGCAGCAAAGCT; GGCACCGGTGGTTCCTCAGGCTATCACTACCTGCGATCAACTGTGAGTGATAGGTACAAG; GTATTTGTAGATTTATTTAATCTTTCAACATACCTGATTCCCCGACACTGGATACCGAAG; ATGAACCCAACCATTCACAAATTTCTATATACAGCAGAATACTGTGATAGCTCCTACTTC; AGCAGTGATGAATCAGATTAA; ; Clone variation with respect to NM_005651.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005651 unedited; NGGGCGTGTCAGAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGCCTA; GAGTCAAACCTCCGTGCTTCTCAGACGTGCCTTTTCACCATGAGTGGGTGCCCATTTTTA; GGAAACAACTTTGGATATACTTTTAAAAAACTCCCCGTAGAAGGCAGCGAAGAAGACAAA; TCACAAACTGGTGTGAATAGAGCCAGCAAAGGAGGTCTTATCTATGGGAACTACCTGCAT; TTGGAAAAAGTTTTGAATGCACAAGAACTGCAAAGTGAAACAAAAGGAAATAAAATCCAT; GATGAACATCTTTTTATCATAACTCATCAAGCTTATGAACTCTGGTTTAAGCAAATCCTC; TGGGAGTTGGATTCTGTTCGAGAGATCTTTCAGAATGGCCATGTCAGAGATGAAAGGAAC; ATGCTTAAGGTTGTTTCTCGGATGCACCGAGTGTCAGTGATCCTGAAACTGCTGGTGCAG; CAGTTTTCCATTCTGGAGACGATGACAGCCTTGGACTTCAATGACTTCAGAGAGTACTTA; TCTCCAGCATCAGGCTTCCAGAGTTTGCAATTCCGACTATTAGAAAACAAGAATAGGTGT; TCTTCAGAACATGAGAGTCCCTTATAACAGAAGACATTATCGTGATAACTTCAAAGGAGA; AGAAAATGAACTGCTACTTAAATCTGAGCANGAAAAGACACTTCTGGAATTAGTGGANGC; ATGGCTGGAAAGAACTNCAGGTTTAGAGCCCATGGATTTAACTTCTGGGGAAAGCTTGAA; AAAATATCACCAGAGGCCTGNAAAGAGAANTCATTAGGATTCANNGCTAAGAAGAGTCTG; AAGAAAAGAGNAACAANGTGGNCTGATTTCAGAGCAANAAGAAGTGCTACTNGTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005651 unedited; NCCCACGTTGAAGCGCGGTCCGCATGCTAGGTACAAGCCTGGGGTAGGGTCACATGGTAG; GGGCGCAAGAATTCAGACTTGAATATTTTAAAAATTTGTTCGAAAGCCTTAGTCCAGAAT; AATATAATGTACTGCATAAAAATTTGAAATAAATGAAGTTTACAAGTTTATAAAGATGAA; ACAATGAGATAGTGATTACATTAAGTGGTTACGTTAAGTCTCAGGGGAAACTGAATTTTT; AATAGGAAAACACCCTATGTACAATGTTACTATTTAAATGTATTCTTTCTACATCTAAAT; GCTAAGTCTTGCCGCAAACAAAAGGTAATCAAATTGTTTCTAGAATGTAAATCATAGCAT; CGTGGTGCTGAACAATATATGCATACATATTTTCAAACTGTATTTATGAAAATCCTTAGA; AAATAAAAAATAGGCTGTGAAACCAGGCTTCTTCCATAGATTTTGCAGACGATTTTAATC; GGATTCATCACTGCTGAAGTAGGAGCTATCACAGTCTTCAGCTGCATATAGAAATTAGAG; AATGGCTGGGTTCATCTTCGGTATCCAGTGTCGGGGAATCAGGTCTGTTGAAAGATTAAG; TCAGTCTACAGATACCTTGTACCGATCACTCACAGTTGATCGCAGGTAGTGATAGCCTGC; AGGACCACCGGAGCCATCTTTGCTGCCCCGCATTCTGTGCACCATGCACACATGGTTATA; TCTCCTTTGATCACCAGGAAAACCAGTGCCTCAGATAATCCGCCCCTGAAAAGGCGCCTG; AAACCTAGGCTGTTTCCTGGTAAAATGGTTCATTCATGCATCCTGGACTGCTCAGAATGA; CAGCCCTTCTTTACCCTTCCTAAAGAAAAGGTCATGACGTTACAAAACAAAAAAGGGACC; TGCCGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_005651
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005651.1, NP_005642.1
• RefSeq Size: 1712 bp
• RefSeq ORF: 1221 bp
• Locus ID: 6999
• UniProt ID: P48775
• Cytogenetics: 4q32.1
• Domains: Trp_dioxygenase
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• Summary: This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]