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NPC2 (NM_006432) Human Untagged Clone

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SKU
SC116115
NPC2 (untagged)-Human Niemann-Pick disease, type C2 (NPC2)
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NPC2
Synonyms EDDM1; HE1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116115 sequence for NM_006432 edited (data generated by NextGen Sequencing)
ATGCGTTTCCTGGCAGCTACATTCCTGCTCCTGGCGCTCAGCACCGCTGCCCAGGCCGAA
CCGGTGCAGTTCAAGGACTGCGGTTCTGTGGATGGAGTTATAAAGGAAGTGAATGTGAGC
CCATGCCCCACCCAACCCTGCCAGCTGAGCAAAGGACAGTCTTACAGCGTCAATGTCACC
TTCACCAGCAATATTCAGTCTAAAAGCAGCAAGGCCGTGGTGCATGGCATCCTGATGGGC
GTCCCAGTTCCCTTTCCCATTCCTGAGCCTGATGGTTGTAAGAGTGGAATTAACTGCCCT
ATCCAAAAAGACAAGACCTATAGCTACCTGAATAAACTACCAGTGAAAAGCGAATATCCC
TCTATAAAACTGGTGGTGGAGTGGCAACTTCAGGATGACAAAAACCAAAGTCTCTTCTGC
TGGGAAATCCCAGTACAGATCGTTTCTCATCTCTAA

Clone variation with respect to NM_006432.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006432 unedited
ATATTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCCTGACTGGGCT
CCTCCCCGGGCCCGCCCCGACGGTTTGTCTTGTGACCGCGGGCGGCCGCTGCTTCTTTCC
CGAGCTTGGAACTTCGTTATCCGCGATGCGTTTCCTGGCAGCTACATTCCTGCTCCTGGC
GCTCAGCACCGCTGCCCAGGCCGAACCGGTGCAGTTCAAGGACTGCGGTTCTGTGGATGG
AGTTATAAAGGAAGTGAATGTGAGCCCATGCCCCACCCAACCCTGCCAGCTGAGCAAAGG
ACAGTCTTACAGCGTCAATGTCACCTTCACCAGCAATATTCAGTCTAAAAGCAGCAAGGC
CGTGGTGCATGGCATCCTGATGGGCGTCCCAGTTCCCTTTCCCATTCCTGAGCCTGATGG
TTGTAAGAGTGGAATTAACTGCCCTATCCAAAAAGACAAGACCTATAGCTACCTGAATAA
ACTACCAGTGAAAAGCGAATATCCCTCTATAAAACTGGTGGTGGAGTGGCAACTTCAGGA
TGACAAAAACCAAAGTCTCTTCTGCTGGGAAATCCCAGTACAGATCGTTTCTCATCTCTA
AGTGCCTCATTGAGTTCGGTGCATCTGGCCAATGAGTCTGCTGAGACTCTTGACAGCACC
TCCAGCTCTGCTGCTTCAACAACAGTGACTTGCTCTCCAATGGTATCCCAGTGATTCGTT
GAAGAGGAGGTGCTCTGTAGCAGAAACTGAGCTCCGGGTGGCTGGTTCTCAGTGGTTGNC
TCATGTCTCTTTTTCTGTCTTAAGNGGNTTCATTAATGCAGCACTTGGNTAGCAGATGTT
TATTTTTTTTTTAACACATAAACTTGTGGCCTCTTCTACACCTGGAATTTACTCTGAATA
ATAAAACTCGTTGTCCTGTCTCTGCAAAAAAAAAAAAAACTCGCTCTAGATGCGGCCGCG
TCAAGCTGTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006432 unedited
GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCAGAAGACAGACAAACGAG
TTTTTATTTATTCAAGAGTAAATTTCCAGGTGTAGAAAGAGGCCACAAGTTAATGTTGTT
AAAAAAAAAATTAAACATCTGCTAACCAAGTGCTGCATTTAATGAAACCACCTAAGACAG
AAAAAGAGACATGAGACAACCACTGAGAACCAGCCACCCGGAGCTCAGTTTCTGCTACAG
AGCACCTCCTCTTCAACGAATCACTGGATACCATTGGAGAGCAAGTCACTGTTGTTGAAG
CAGCAGAGCTGGAGGTGCTGTCAAGAGTCTCAGCAGACTCATTGGCCAGATGCACCGAAC
TCAATGAGGCACTTAGAGATGAGAAACGATCTGTACTGGGATTTCCCAGCAGAAGAGACT
TTGGTTTTTGTCATCCTGAAGTTGCCACTCCACCACCAGTTTTATAGAGGGATATTCGCT
TTTCACTGGTAGTTTATTCAGGTAGCTATAGGTCTTGTCTTTTTGGATAGGGCAGTTAAT
TCCACTCTTACAACCATCAGGCTCAGGAATGGGAAAGGGAACTGGGACGCCCATCAGGAT
GCCATGCACCACGGCCTTGCTGCTTTTAGACTGAATATTGCTGGTGAAGGTGACATTGAC
GCTGTAAGACTGTCCTTTGCTCANCTGGCANGGGTTGGGTGGGGCATGGGCTCACATTCA
CTTCCTTTATACTNCATCCACAGACCGCAGTCCTTTGACTGCACCCGGTTCGCCTGGGNN
CACGTGCTGNACGCCAGGACAGGAATGTANCTGCAGGAAACGCATCGCGGATAACGAAAG
TCCCAGCTCGGGAAAGAAGCACGGCCGCCCGCGTCACAAGACAAACTGTCGGGGCCGCCC
GGGNAGGGACCCAATCAGGCTGGTGCGAAATCCGGNCGCCCTTAGTGGAGTCGATACA
Restriction Sites NotI-NotI |
ACCN NM_006432
Insert Size 850 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006432.3, NP_006423.1
RefSeq Size 921 bp
RefSeq ORF 456 bp
Locus ID 10577
UniProt ID P61916
Cytogenetics 14q24.3
Domains ML
Protein Families Druggable Genome, Secreted Protein
Summary This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006432" in other vectors (6)
SKU Description Size Price
RC200282 NPC2 (Myc-DDK-tagged)-Human Niemann-Pick disease, type C2 (NPC2) 10 ug
$150.00
RC200282L1 Lenti ORF clone of Human Niemann-Pick disease, type C2 (NPC2), Myc-DDK-tagged 10 ug
$450.00
RC200282L2 Lenti ORF clone of Human Niemann-Pick disease, type C2 (NPC2), mGFP tagged 10 ug
$450.00
RC200282L3 Lenti ORF clone of Human Niemann-Pick disease, type C2 (NPC2), Myc-DDK-tagged 10 ug
$450.00
RC200282L4 Lenti ORF clone of Human Niemann-Pick disease, type C2 (NPC2), mGFP tagged 10 ug
$450.00
RG200282 NPC2 (tGFP-tagged) - Human Niemann-Pick disease, type C2 (NPC2) 10 ug
$489.00

file-alt Citations

NPC2 facilitates bidirectional transfer of cholesterol between NPC1 and lipid bilayers, a step in cholesterol egress from lysosomes
Infante, Wang, Radhakrishnan et al
Proc Natl Acad Sci U S A (2008) 105 (40), 15287-92 DOI: 10.1073/pnas.0807328105
Cited by 497
AI Summary: In this study, OriGene’s human NPC2 cDNA (OriGene, SC122208) was used to express and analyze the function of NPC2 protein in cholesterol transfer assays, helping to elucidate its role in facilitating cholesterol movement between NPC1 and lipid bilayers.
Purified NPC1 protein: II. Localization of sterol binding to a 240-amino acid soluble luminal loop
Infante, Radhakrishnan, Abi-Mosleh et al
J Biol Chem (2008) 283 (2), 1064-75 DOI: 10.1074/jbc.M707944200
AI Summary: OriGene's products, specifically the Q79A mutant NPC1 cDNA (OriGene, SC120746), were used to express and analyze mutant NPC1 protein, enabling the authors to determine the role of the luminal loop-1 sterol binding site in cholesterol binding and transport.
Showing 1-2 of 2 papers.
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