ARPP19 (NM_006628) Human Untagged Clone

SKU SC115993

ARPP19 (untagged)-Human cAMP-regulated phosphoprotein, 19kDa (ARPP19)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARPP19
• Synonyms: ARPP-16; ARPP-19; ARPP16; ENSAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115993 sequence for NM_006628 edited (data generated by NextGen Sequencing); ATGTCTGCGGAAGTCCCCGAGGCAGCCTCCGCGGAGGAGCAGAAGGAAATGGAAGATAAA; GTGACTAGTCCAGAGAAAGCAGAAGAAGCAAAATTAAAAGCAAGATATCCTCATCTGGGA; CAAAAGCCTGGAGGTTCAGATTTCTTAAGGAAACGGTTGCAGAAAGGGCAAAAATATTTT; GATTCTGGGGATTACAACATGGCTAAAGCAAAAATGAAGAACAAGCAACTTCCTACTGCA; GCTCCGGATAAGACGGAGGTCACTGGTGACCACATTCCCACTCCGCAAGACCTTCCTCAA; CGGAAGCCGTCCCTTGTTGCTAGCAAGCTGGCTGGCTGA; ; Clone variation with respect to NM_006628.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006628 unedited; GTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCATTTTCGCG; GGCGGAGGCGGCCCGGCGGGCCCTGGGAGAGCTGGGACGGGCGGCGGCCGGGTGGCCTCG; GCCACCCGCTAATTGCATCTTTTCCCGGCGTCTCGTCTGCAGAGGGAGCACTATGTCTGC; GGAAGTCCCCGAGGCAGCCTCCGCGGAGGAGCAGAAGGAAATGGAAGATAAAGTGACTAG; TCCAGAGAAAGCAGAAGAAGCAAAATTAAAAGCAAGATATCCTCATCTGGGACAAAAGCC; TGGAGGTTCAGATTTCTTAAGGAAACGGTTGCAGAAAGGGCAAAAATATTTTGATTCTGG; GGATTACAACATGGCTAAAGCAAAAATGAAGAACAAGCAACTTCCTACTGCAGCTCCGGA; TAAGACGGAGGTCACTGGTGACCACATTCCCACTCCGCAAGACCTTCCTCAACGGAAGCC; GTCCCTTGTTGCTAGCAAGCTGGCTGGCTGATTAAGAGGCTGAACTGCATGAATCTGCTA; AATCTCATTATTTCTCCTTAATATGTTACTTATCTACTTTTTATTTCCTTTCATTCACTA; GTCATTTGAGACTGACAGCTTTGCAGGTAGCAGTAGTGTGTGCTGCTATTGTGGAATATA; CGTGTGTAGAGTTTTTGATTAGTTTAACAGTGCACTGGTGAAGAGGACATGTTAGAGCAA; CATAAGTAAACTACTTGAAATAGTTGTATATATTACCTAACTTCTAGTGTAGTACTGGTT; CTAACAAGTAACCAAGCAGTTTTAANAATTTAATGTTTTGGCTNTCATTACTTCATCTTA; ATTATAGCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006628
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006628.4, NP_006619.1
• RefSeq Size: 5162 bp
• RefSeq ORF: 339 bp
• Locus ID: 10776
• UniProt ID: P56211
• Cytogenetics: 15q21.2
• Domains: endosulfine
• Protein Families: Druggable Genome
• Summary: The 19-kD cAMP-regulated phosphoprotein plays a role in regulating mitosis by inhibiting protein phosphatase-2A (PP2A; see MIM 176915) (summary by Gharbi-Ayachi et al., 2010 [PubMed 21164014]).[supplied by OMIM, Feb 2011]; Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the CDS. The encoded isoform (2) is shorter than isoform 1. Both variants 3 and 4 encode isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.