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ME3 (NM_006680) Human Untagged Clone

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SKU
SC115951
ME3 (untagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1
  $563.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ME3
Synonyms NADP-ME
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115951 sequence for NM_006680 edited (data generated by NextGen Sequencing)
ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGC
GCCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCG
GCGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAAC
AAGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCG
CCCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCATGAGATATTACGAGCGGCAG
CAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTC
TTCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC
GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATC
ACCATTCATGACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATT
AAGGCCGTGGTGGTGACTGATGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTAC
GGCATGGGCATCCCTGTGGGCAAGCTGGCCCTGTACACGGCATGCGGAGGGGTGAACCCG
CAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACAATGAGGAGCTGCTCAGAGAC
CCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGATGACTTGCTG
GATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA
GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATG
TTCAATGATGACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTG
CGAATCACCAACAACAAGCTTTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCA
GCTATGGGCATTGCCCACCTCCTTGTCATGGCCCTAGAGAAAGAAGGTGTACCGAAGGCA
GAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGCTCATTGTCAAGGGGAGGAGC
CACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAACTCCCTGGAG
GAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC
TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCC
CTGAGCAACCCCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAG
GGCCGAGGGATTTTTGCCAGTGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAG
ACCTTCATTCCTGGGCAGGGAAACAATGCTTACGTGTTCCCCGGGGTGGCACTGGGAGTC
ATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCCTCCTGACAGCAGAGCAAATT
GCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTATCCACCACTCAGCACC
ATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC
CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTAC
ACTCCAGACTATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAAT
GTTCAGACGGTCTGA

Clone variation with respect to NM_006680.2
972 g=>c;1605 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006680 unedited
GGGTCAATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTCAGGCG
GGCATTCTGAGCTGCTTCTTGTCACTGGGNGAAAACCACGTGCACGCAGTGCTGAGGCGC
CCCACGCGCGTCCTCAGGGGAAGCCAGGCACTGAGAACAAAGGTTGTGGGACCGCTCTTT
GAGGAACTCACCAGCTCCGCGAGCTCGCGGCCGCCAGCGCGGTCCACCCATTGGCGGGAG
AGGACTCACAGACCGCAGCTCCTCACCCCGCCTGCTCCGCAGCGGGACCGCGTAGACCTC
AGCTCCCCGAGCAGCGCAGGTGGGCCAGGGAAGGAGAGGACCGAGGTCTGCCAAGGACCA
TGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCG
CCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGG
CGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACA
AGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCGC
CCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCATGAGATATTACGAGCGGCAGC
AGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTCT
TCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACCG
TGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCA
CCATTCATGACANAGGTCATCTTTGCACAATGCTGAATCTTGGCCAGAAGACATATTAGG
CA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006680 unedited
GTACGCGGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTCAAAAACAAG
GCTTTTATTCACTGTATGCCTTGGCATCTCCTCTCACACCAAAAAGTTTAAAAGAAAAAA
CACAGCGACAAGGATTCACTTGGGCCATTTTGGAACCCATTTATATTGGGGGTGTTACTC
TGGGCTTCATCTAGCCCCATACTAGCCTCTGAAACTGCCTCAAACCGTCTGAACATTCAT
GGCTTCCTTGGGCCAAGTGTAGCTGTCCAGTGTAAAGGAGTCATAGTCTGGAGTGTAAAC
CAGGGATCTTACAAAAGCCTCCTTGTCCTTAGGCTCTGGGTAGTAGGAAGCCAGGTTGTG
TTTGTACGCGTAGTCGAAAACTTTGATGGCAATTCTCAAAGACACGTCTCGGATGGTGCT
GAGTGGTGGATAAAGTCTCCCCTGGGACAGATGCTGCTCAAAGACTTCCTGGGCAATTTG
CTCTGCTGTCAGGAGGAAAATCTCATCTGGGATGTGCCGGATCCCGCCGGCGATGACTCC
CAGTGCCACCCCGGGGAACACGCAAGCATTGTTTCCCTGCCCAGGAATGAAGGTCTTGCC
ATCTTCCAAAGCCACACTCTTAAAAGGACTTCCACTGGAAAAAATCCTTCGGTCCTTCGG
AGACCCGGAAGAACTTATAAACCGCGCACTTCGGCCTTGCTGGGGGGGTTCTCCAGGGCA
AAAATAATAGGCCGCTCCGGGAAGGAGGCCCTGTCCCTCAAAATCTGCTCCGTGAAAGCC
TCCTGCTATGGCAGACACACCTATGTAGGGCTGTGGGCTTACACAGCCCTACCCACTTCT
CCAGGGAGTCTATTTAAGAATGGCCTTGGCACAACTTTCCTTTTATGGTCCAGGGGGTTC
CCCCTCACAAAGACACCTCTAAG
Restriction Sites NotI-NotI |
ACCN NM_006680
Insert Size 2260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006680.2, NP_006671.2
RefSeq Size 2090 bp
RefSeq ORF 1815 bp
Locus ID 10873
UniProt ID Q16798
Cytogenetics 11q14.2
Domains malic
Protein Pathways Metabolic pathways, Pyruvate metabolism
Summary Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) and variants 2-4 all encode the same protein.
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Documents
Resources
"NM_006680" in other vectors (6)
SKU Description Size Price
RC224151 ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$289.00 $562.00
RC224151L1 Lenti-ORF clone of ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RC224151L2 Lenti-ORF clone of ME3 (mGFP-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RC224151L3 Lenti-ORF clone of ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RC224151L4 Lenti-ORF clone of ME3 (mGFP-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RG224151 ME3 (tGFP-tagged) - Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00 $762.00

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