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ME3 (NM_006680) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG224151
ME3 (tGFP-tagged) - Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $762.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ME3
Synonyms NADP-ME
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG224151 representing NM_006680
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCGCCCTCCCGC
GCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGGCGCGCCCTGTGCCCCTGAA
GAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACAAGGGGATGGCCTTTACCCTTGAAGAAAGG
CTGCAGCTTGGAATCCACGGCCTAATCCCGCCCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCA
TGAGATATTACGAGCGGCAGCAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAA
CGAGAAGCTCTTCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC
GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCACCATTCATG
ACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATTAAGGCCGTGGTGGTGACTGA
TGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTACGGCATGGGCATCCCTGTGGGCAAGCTGGCC
CTGTACACGGCATGCGGAGGGGTGAACCCGCAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACA
ATGAGGAGCTGCTCAGAGACCCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGA
TGACTTGCTGGATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA
GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATGTTCAATGATG
ACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTGCGAATCACCAAGAACAAGCT
TTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCAGCTATGGGCATTGCCCACCTCCTTGTCATG
GCCCTAGAGAAAGAAGGTGTACCGAAGGCAGAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGC
TCATTGTCAAGGGGAGGAGCCACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAA
CTCCCTGGAGGAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC
TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCCCTGAGCAACC
CCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAGGGCCGAGGGATTTTTGCCAG
TGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAGACCTTCATTCCTGGGCAGGGAAACAATGCT
TACGTGTTCCCCGGGGTGGCACTGGGAGTCATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCC
TCCTGACAGCAGAGCAAATTGCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTACCCACC
ACTCAGCACCATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC
CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTACACTCCAGACT
ATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAATGTTCAGACGGTC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_006680
ORF Size 1812 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006680.2, NP_006671.2
RefSeq Size 2090 bp
RefSeq ORF 1815 bp
Locus ID 10873
UniProt ID Q16798
Cytogenetics 11q14.2
Domains malic
Protein Pathways Metabolic pathways, Pyruvate metabolism
Summary Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006680" in other vectors (6)
SKU Description Size Price
RC224151 ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$289.00 $562.00
RC224151L1 Lenti-ORF clone of ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RC224151L2 Lenti-ORF clone of ME3 (mGFP-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RC224151L3 Lenti-ORF clone of ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
RC224151L4 Lenti-ORF clone of ME3 (mGFP-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$862.00
SC115951 ME3 (untagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$563.00

Plasmid Map for RG224151

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG224151
Circular map for RG224151

file-alt Citations

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