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EAP30 (SNF8) (NM_007241) Human Untagged Clone

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SKU
SC115610
SNF8 (untagged)-Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EAP30
Synonyms Dot3; EAP30; VPS22
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_007241, the custom clone sequence may differ by one or more nucleotides


ATGCACCGCCGCGGGGTGGGAGCTGGCGCCATCGCCAAGAAGAAACTTGCAGAGGCCAAGTATAAGGAGC
GAGGGACGGTCTTGGCTGAGGACCAGCTAGCCCAGATGTCAAAGCAGTTGGACATGTTCAAGACCAACCT
GGAGGAATTTGCCAGCAAACACAAGCAGGAGATCCGGAAGAATCCTGAGTTCCGTGTGCAGTTCCAGGAC
ATGTGTGCAACCATTGGCGTGGATCCGCTGGCCTCTGGAAAAGGATTTTGGTCTGAGATGCTGGGCGTGG
GGGACTTCTATTACGAACTAGGTGTCCAAATTATCGAAGTGTGCCTGGCGCTGAAGCATCGGAATGGAGG
TCTGATAACTTTGGAGGAACTACATCAACAGGTGTTGAAGGGAAGGGGCAAGTTCGCCCAGGATGTCAGT
CAAGATGACCTGATCAGAGCCATCAAGAAACTAAAGGCACTTGGCACTGGCTTCGGCATCATCCCTGTGG
GCGGCACTTACCTCATTCAGTCTGTTCCAGCTGAGCTCAATATGGATCACACCGTGGTGCTGCAGCTGGC
AGAGAAGAATGGCTACGTGACTGTCAGTGAGATCAAAGCCAGTCTTAAATGGGAGACCGAGCGAGCGCGG
CAAGTGCTGGAACACCTGCTGAAGGAAGGGTTGGCGTGGCTGGACTTACAGGCCCCAGGGGAGGCCCACT
ACTGGCTGCCAGCTCTCTTCACTGACCTCTACTCCCAGGAGATTACAGCTGAGGAGGCCAGAGAAGCCCT
CCCCTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_007241 unedited
CATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGAGTCC
GGGACGTGGAGACCCGGGGTCCCGGCAGCCGGGCGGCCCGCGGGCCCAGGGTGGGGATGC
ACCGCCGCGGGGTGGGAGCTGGCGCCATCGCCAAGAAGAAACTTGCAGAGGCCAAGTATA
AGGAGCGAGGGACGGTCTTGGCTGAGGACCAGCTAGCCCAGATGTCAAAGCAGTTGGACA
TGTTCAAGACCAACCTGGAGGAATTTGCCAGCAAACACAAGCAGGAGATCCGGAAGAATC
CTGAGTTCCGTGTGCAGTTCCAGGACATGTGTGCAACCATTGGCGTGGATCCGCTGGCCT
CTGGAAAAGGATTTTGGTCTGAGATGCTGGGCGTGGGGGACTTCTATTACGAACTAGGTG
TCCAAATTATCGAAGTGTGCCTGGCGCTGAAGCATCGGAATGGAGGTCTGATAACTTTGG
AGGAACTACATCAACAGGTGTTGAAGGGAAGGGGCAAGTTCGCCCAGGATGTCAGTCAAG
ATGACCTGATCAGAGCCATCAAGAAACTAAAGGCACTTGGCACTGGCTTCGGCATCATCC
CTGTGGGCGGCACTTACCTCATTCAGTCTGTTCCAGCTGAGCTCAATATGGATCACACCG
TGGTGCTGCAGCTGGCAGAGAAGAATGGCTACGTGACTGTCAGTGAGATCAAAGCCAGTC
TTAAATGGGAGACCGAGCGAGCGCGGGCAGTGCTGGAACACCTGCTAAAGGAGGNTTTNG
CGTGCTGGACTACAGGCCCCAGGGNAGGCCACTACTGGCTGCCAGCTCTCTTCACTGACC
TCTACTCCCCAGAGATACAGCTGAGGAGGCCAGANAAGCCTNCCCTGACTGCATGTGGAG
GGCCACANCANCAGGCAGGGAGGAGGCNGAGGTGGCAAATAAACTGGGCATTTTTGTNAT
TCAAAAATAAAAAAAAN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_007241 unedited
ACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTATTTTTGGGAAAAACAAAATTGCCCAAGTTTATTTGCCACCTCCGCCTC
CTCCCTGCCTGGTGGTGGGGGCCCTTCCACATGCAGTCAGGGGAGGGCTTTTTTGGCCTC
CTCAACTGTAATCTCCTGGGAGTAAAGGTCAATGAAAAAAACTGGCAGCCATTATTGGGC
CTCCCCTGGGGCCTGTAAGTCCAACCACCCCAACCCTTTCTTTAACAAGGGTTCCAACAC
TTGCCGGGCTCGCTCGGGCTCCCATTTAAAAATGGCTTTGATCTCACTGACAGTCACGTA
ACCATTTTTTTTTGCCAGCTGGAGCACCACGGGGGGATCCATATTGAGCTCACCTGGAAC
AAACTGAATGAGGTAAGTGCCCCCCACAGGGATGATGCCCAATCCAGTGCCAAGGGCCTT
TAATTTCTTGAAGGCTCTGATCAAGGCATCTTGACTGACATCCTGGGCGAACTTGCCCCT
TCCCTTCAACACCTGTTGATGTAGTTCCTCCAAAGTTATCAAACCTCCATTCCGATGCTT
CAACGCCAGGCACACTTCGATAATTTGGACACCTATTTCGTAATAGAAGTCCCCCACGCC
CAGCATCTCAGACCAAAATCCTTTTCCAGAGGCCCGCGGATCCACGCCAATGGTTGCACA
CATGTCCTGGAACTGCCACGGAACTCAAGATTCTTCCGGACTCCCGCTTGGGTTGCTGGC
AAATCCTCAAGTTGTCTGAACAGTCCACTGTTTGAATCTGGCTACTGGCCTACCAGACCG
CCTCCTCTTAACTGGCCTTGGAATTTTTCTGGGATGCGCACTCCCCCCGGGGGGGTTCCC
CCTGGCCGGGGCCCCCGTGCGGAACCCGGTTCACTTCGTCTCCCCCTTTGCAATTCGG
Restriction Sites NotI-NotI |
ACCN NM_007241
Insert Size 1110 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007241.2, NP_009172.2
RefSeq Size 1226 bp
RefSeq ORF 777 bp
Locus ID 11267
UniProt ID Q96H20
Cytogenetics 17q21.32
Domains EAP30
Protein Families Transcription Factors
Protein Pathways Endocytosis
Summary The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2015
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_007241" in other vectors (7)
SKU Description Size Price
RC202137 SNF8 (Myc-DDK-tagged)-Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8) 10 ug
$300.00
RC202137L1 Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), Myc-DDK-tagged 10 ug
$600.00
RC202137L2 Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), mGFP tagged 10 ug
$600.00
RC202137L3 Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), Myc-DDK-tagged 10 ug
$600.00
RC202137L4 Lenti ORF clone of Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8), mGFP tagged 10 ug
$600.00
RG202137 SNF8 (tGFP-tagged) - Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8) 10 ug
$489.00 $500.00
SC322220 SNF8 (untagged)-Human SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) (SNF8) 10 ug
$300.00

file-alt Citations

Involvement of ESCRT-II in Hepatitis B Virus Morphogenesis
Stieler, Prange
PLoS ONE (2014) 9 (3), e91279 DOI: 10.1371/journal.pone.0091279
Cited by 65
AI Summary: In this study, a human EAP20 cDNA construct from OriGene was used to investigate the role of ESCRT-II components in hepatitis B virus morphogenesis.
Snippet: employed [38]. Full-length cDNA clones encoding human EAP30 (NM_007241) and EAP45 (NM_016075) were obtained from imaGenes (Germany), while a human EAP20 (NM_032353) construct was purchased from Origene. For ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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