CD2AP (NM_012120) Human Untagged Clone

SKU SC115518

CD2AP (untagged)-Human CD2-associated protein (CD2AP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD2AP
• Synonyms: CMS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012120, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGTTGACTATATTGTGGAGTATGACTATGATGCTGTACATGATGATGAATTAACTATTCGAGTTGGAG; AAATCATCAGGAATGTGAAAAAGCTACAGGAGGAAGGGTGGCTGGAAGGAGAACTAAATGGGAGAAGAGG; AATGTTCCCTGACAATTTCGTTAAGGAAATTAAAAGAGAGACGGAATTCAAGGATGACAGTTTGCCCATC; AAACGGGAAAGGCATGGGAATGTAGCAAGTCTTGTACAACGAATAAGCACCTATGGACTTCCAGCTGGAG; GAATTCAGCCACATCCACAAACCAAAAACATTAAGAAGAAGACCAAGAAGCGTCAGTGTAAAGTTCTTTT; TGAGTACATTCCACAAAATGAGGATGAACTGGAGCTGAAAGTGGGAGATATTATTGATATTAATGAAGAG; GTAGAAGAAGGCTGGTGGAGTGGAACCCTGAATAACAAGTTGGGACTGTTTCCCTCAAATTTTGTGAAAG; AATTAGAGGTAACAGATGATGGTGAAACTCATGAAGCCCAGGACGATTCAGAAACTGTTTTGGCTGGGCC; TACTTCACCTATACCTTCTCTGGGAAATGTGAGTGAAACTGCATCTGGATCAGTTACACAGCCAAAGAAA; ATTCGAGGAATTGGATTTGGAGACATTTTTAAAGAAGGCTCTGTGAAACTTCGGACAAGAACATCCAGTA; GTGAAACAGAAGAGAAAAAACCAGAAAAGCCCTTAATCCTACAGTCACTGGGACCCAAAACTCAGAGTGT; GGAGATAACAAAAACAGATACCGAAGGTAAAATTAAAGCTAAAGAATATTGTAGAACATTATTTGCCTAT; GAAGGTACTAATGAAGATGAACTTACTTTTAAAGAGGGGGAGATAATCCATTTGATAAGTAAGGAGACTG; GAGAAGCTGGCTGGTGGAGGGGCGAACTTAATGGTAAAGAAGGAGTATTTCCAGACAATTTTGCTGTCCA; GATAAATGAACTTGATAAAGACTTTCCAAAACCAAAGAAACCACCACCTCCTGCTAAGGCTCCAGCTCCA; AAGCCTGAACTGATAGCTGCAGAGAAGAAATATTTTTCTTTAAAGCCTGAAGAAAAGGATGAAAAATCAA; CACTGGAACAGAAACCTTCTAAACCAGCAGCTCCACAAGTCCCACCCAAGAAACCTACTCCACCTACCAA; AGCCAGTAATTTACTGAGATCTTCTGGAACAGTGTACCCAAAGCGACCTGAAAAACCAGTTCCTCCACCA; CCTCCTATAGCCAAGATTAATGGGGAAGTTTCTAGCATTTCATCAAAATTTGAAACTGAGCCAGTATCAA; AACTAAAGCTAGATTCTGAACAGCTGCCCCTTAGACCAAAATCAGTAGACTTTGATTCACTTACAGTAAG; GACCTCCAAAGAAACAGATGTTGTAAATTTTGATGACATAGCTTCCTCAGAAAACTTGCTTCATCTCACT; GCAAATAGACCAAAGATGCCTGGAAGAAGGTTGCCGGGCCGTTTCAATGGTGGACATTCTCCAACTCACA; GCCCCGAAAAAATCTTGAAGTTACCAAAAGAAGAAGACAGTGCCAACCTGAAGCCATCTGAATTAAAAAA; AGATACATGCTACTCTCCAAAGCCATCTGTGTACCTTTCAACACCTTCCAGTGCTTCTAAAGCAAATACA; ACTGCTTTCCTGACTCCATTAGAAATCAAAGCTAAAGTGGAAACAGATGATGTGAAAAAAAATTCCCTGG; ATGAACTTAGAGCCCAGATTATTGAATTGTTGTGCATTGTAGAAGCACTGAAAAAGGATCACGGGAAAGA; ACTGGAAAAACTGCGAAAAGATTTGGAAGAAGAGAAGACAATGAGAAGTAATCTAGAGATGGAAATAGAG; AAGCTGAAAAAAGCTGTCCTGTCTTCTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012120 unedited; GTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCAGCATGGTTG; ACTATATTGTGGAGTATGACTATNATGCTGTACATGATGATGAATTAACTATTCGAGTTG; GAGAAATCATCAGGAATGTGAAAAAGCTACAGGAGGAAGGGTGGCTGGAAGGAGAACTAA; ATGGGAGAAGAGGAATGTTCCCTGACAATTTCGTTAAGGAAATTAAAAGAGAGACGGAAT; TCAAGGATGACAGTTTGCCCATCAAACGGGAAAGGCATGGGAATGTAGCAAGTCTTGTAC; AACGAATAAGCACCTATGGACTTCCAGCTGGAGGAATTCAGCCACATCCACAAACCAAAA; ACATTAAGAAGAAGACCAAGAAGCGTCAGTGTAAAGTTCTTTTTGAGTACATTCCACAAA; ATGAGGATGAACTGGAGCTGAAAGTGGGAGATATTATTGATATTAATGAAGAGGTAGAAG; AAGGCTGGTGGAGTGGAACCCTGAATAACAAGTTGGGACTGTTTCCCTCAAATTTTGTGA; AAGAATTAGAGGTAACAGATGATGGTGAAACTCATGAAGCCCAGGACGATTCAAAAACTG; TTTTGGCTGGGCCTACTTCACCTATACCTTCTCTGGGAAATGTGAGTGAAACTGCATCTG; GTTCAGTTACACAGCCAAAGAAAATTCGAGGAATTGGATTTGGAGACATTTTTAAAGAAG; GCTCTGTGANACTTNCGGACAGACATCCAGTAGTGAAACAGAAGAGAAAAACCAGAAAGC; CCTTATCCTACAGTCACTGGGACCCANACTCANAGTGTGGAGATNACANAACAGATACCG; AAGTAAAATTAAGCTAAAGATATTGTAGACATTATTGCTATGANGGTACTAATGAGAGAA; CTACTTTAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012120 unedited; ACATTCAACTGGGCATTATGTAACANNGTTATTGAAAGCAAGTCAGGGTAAAATTTTATA; GCCAAAAATATTCATAGCAGCAACTTTTAAAATATGATTTTATTAAATCAAGTCATTGCA; CTTGGTCATTTTATTGCTACAGCAAAACAAGGCCTTTACATTATAATACTTCTCATTTCT; GATTTAACTGATTGTCTCATTCTGCTCATACATTTCAAGTTTAAATGCAAGCATAAAATG; TTTATCAACAAATCTAGAGAGCACTTGGATTTTTAATTTTTCTGTGATCACAGTAAGGAG; CATAAAAAAGAGTATCTTCTGTTACACAAGGCCTGTTCTCTCTTTACATCTTCAGACTTA; AATTCTGTAGAAGGTAACAGCTTTGTATTAAGACAGAAGCTTAGTGGTCACAAACAAAAA; ATAACACTGAAATACAATTCGGAAATTAATGATACTGTGTGTCTCAAAGAATACCTGAAC; TATACATTCACTAATAATTTGGCAATGAGATTCCATGCTGTTCAACTTTTGTCTTTTATA; CTCACACGTAGAAAAAAAATTCATGATTTACCCCAAGGTTATTATTAAATATCATTTTGT; GATATGAACTNNTAGTATAGAAATTAATACATTTCCCATACCAACAGAAAGTAAAGTGCT; AGAATAAAGGATCATTNTTCCCATCANGCCTGNGTTTGTTTGGGTTTTCTTACTATTTTA; TCAAGGACAGAAAGGGTAAAGTATTTCAGTTACCTTTTCTATTCTGACTGAGGGAGACAC; CAGCAAAAACTTTAAATACATTTCCATGCTCATAAGCATNNCTGATTCATGTACTATTGA; GAAAATGACCACCAAAACTTTAGAACAGCCTGAAACTGCTGNAAACTTCCTCATCCCATA; TAGGATGACCAGGNATCACTTCATNGATTTTCAGGTGAAAAAGCTGAGAATAATNCTAGA; TACAAATTTTTGTTTACCTAGGTTGCCTTAAAGGAAGAGATTATTTTTTTACGAAGAAAT; AATCTTTGCTGNCCTTTACCTTNCCTTTACGAAAGGACATTTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_012120
• Insert Size: 5520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012120.1, NP_036252.1
• RefSeq Size: 5425 bp
• RefSeq ORF: 1920 bp
• Locus ID: 23607
• UniProt ID: Q9Y5K6
• Cytogenetics: 6p12.3
• Domains: SH3
• Protein Families: Druggable Genome
• Summary: This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008]