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PDCD6 (NM_013232) Human Untagged Clone

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SKU
SC115334
PDCD6 (untagged)-Human programmed cell death 6 (PDCD6)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PDCD6
Synonyms ALG-2; ALG2; PEF1B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115334 sequence for NM_013232 edited (data generated by NextGen Sequencing)
ATGGCCGCCTACTCTTACCGCCCCGGCCCTGGGGCCGGCCCTGGGCCTGCTGCAGGCGCG
GCGCTGCCGGACCAGAGCTTCCTGTGGAACGTTTTCCAGAGGGTCGATAAAGACAGGAGT
GGAGTGATATCAGACACCGAGCTTCAGCAAGCTCTCTCCAACGGCACGTGGACTCCCTTT
AATCCAGTGACTGTCAGGTCGATCATATCCATGTTTGACCGTGAGAACAAGGCCGGCGTG
AACTTCAGCGAGTTCACGGGTGTGTGGAAGTACATCACGGACTGGCAGAACGTCTTCCGC
ACGTACGACCGGGACAACTCCGGGATGATCGATAAGAACGAGCTGAAGCAGGCCCTCTCA
GGTTTCGGCTACCGGCTCTCTGACCAGTTCCACGACATCCTCATTCGAAAGTTTGACAGG
CAGGGACGGGGGCAGATTGCCTTCGACGACTTCATCCAGGGCTGCATCGTCCTGCAGAGG
TTGACGGATATATTCAGACGTTACGACACGGATCAGGACGGCTGGATTCAGGTGTCGTAC
GAACAGTACCTGTCCATGGTCTTCAGTATCGTATGA

Clone variation with respect to NM_013232.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_013232 unedited
GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCGGCCTGAG
AGGTCTCTCGTCGCTGCAGGCGCCTCAGCCCAGCCGCGTGCCTTGGCCCATGGCCGCCTA
CTCTTACCGCCCCGGCCCTGGGGCCGGCCCTGGGCCTGCTGCAGGCGCGGCGCTGCCGGA
CCAGAGCTTCCTGTGGAACGTTTTCCAGAGGGTCGATAAAGACAGGAGTGGAGTGATATC
AGACACCGAGCTTCAGCAAGCTCTCTCCAACGGCACGTGGACTCCCTTTAATCCAGTGAC
TGTCAGGTCGATCATATCCATGTTTGACCGTGAGAACAAGGCCGGCGTGAACTTCAGCGA
GTTCACGGGTGTGTGGAAGTACATCACGGACTGGCAGAACGTCTTCCGCACGTACGACCG
GGACAACTCCGGGATGATCGATAAGAACGAGCTGAAGCAGGCCCTCTCAGGTTTCGGCTA
CCGGCTCTCTGACCAGTTCCACGACATCCTCATTCGAAAGTTTGACAGGCAGGGACGGGG
GCAGATTGCCTTCGACGACTTCATCCAGGGCTGCATCGTCCTGCAGAGGTTGACGGATAT
ATTCAGACGTTACGACACGGATCAGGACGGCTGGATTCAGGTGTCGTACGAACAGTACCT
GTCCATGGTCTTCAGTATCGTATGACCCTGGCCTCTCGTGAAGAGCAGCACAACATGGAA
AGAGCCAAAATGTCACAGTTCCTATCTGTGAGGGAATGGAGCACAGGTGCAGNTAGATGC
TGTTCTTCCTTTAGATTTNGNCACGTGGGGACCCAGCTGTACATATGTGGGATAGCTGAT
TAATGGGTTTGCACTGTNATATAGCTTGTATCGTCTATGCAACATTGGATTTGGNGCTGG
CTCATGTGCCATGAGGGAAGGNAATGGTTCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_013232 unedited
GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCACGTCTAACATTCCCTTTA
TTGCTTACGTCACATATTCCAATGAATACAATAAACTCCTTTTTAAAAAAGTAAGGCACA
TGAAAAGCCTTTTGTGTAAGTTCCATGTTATTTTAAAATTCCTATTGGCTAATAAAAGCA
TTTGCACTATAGAACCAGAGACATCTAGAAAAGCACATGATAGATTTTTTTGCAAGCAGA
ATGCCTGAAACATTACATTTACCTCATGGCACAATGAGACAGTCACCAAATCCAATGTCT
GCATTAGAACGATACAGCTACTATTACAGTTGCAAAACCATTAATCAGCTTATCCACATA
TGTACAGCTGGGTCCCCACGTGACAAAATCTAAAGGAAGAACAGCATCTAACTGCACCTG
TGCTCCATTCCCTCACAGATAGGAACTGTGACATTTTGGCTCTTTCCATGTTGTGCTGCT
CTTCACGAGAGGCCAGGGTCATACGATACTGAAGACCATGGACAGGTACTGTTCGTACGA
CACCTGAATCCAGCCGTCCTGATCCGTGTCGTAACGTCTGAATATATCCGTCAACCTCTG
CAGGACGATGCAGCCCTGGATGAAGTCGTCGAAGGCAATCTGCCCCCGTCCCTGCCTGTC
AAACTTCGAATGANGATGTCGTGGAACTGGTCAGAGAGCCCGGTAGCCGAAACCTGAGAG
GCCCTGCTTCAGCTCGTTCTTATCGATCATCCCGGAGTTGTCCGGTCGTACGTGCCGGAG
ACGTTCTGCCAGTTCGTGATGTACTTCCACACCCCGTGAACTCGCTGAAGTTCACGCCGC
CCTTGTCTCACGTCCACCATGATATGATCGACTGACAGCACTGGATTAAGGAGTCCCCTG
CCGTTGGAAAAGACTGCTGAACCTCGGGCTGAATACACTCCTCCGGNCTTACGACCTCTG
AAACGCTCCAAGAAACTTTGTTCGCANGCCCGCCTGCAAAGCCAGGCCGTCCCAGCCC
Restriction Sites NotI-NotI |
ACCN NM_013232
Insert Size 1070 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013232.2, NP_037364.1
RefSeq Size 1148 bp
RefSeq ORF 576 bp
Locus ID 10016
UniProt ID O75340
Cytogenetics 5p15.33
Domains EFh
Protein Families Druggable Genome
Summary This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. provided by RefSeq, May 2012
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species. An upstream in-frame start codon is also present but is poorly conserved; use of the upstream start codon would result in a protein that is 32 aa longer. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon.
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Documents
Resources
"NM_013232" in other vectors (4)
SKU Description Size Price
RC202030 PDCD6 (Myc-DDK-tagged)-Human programmed cell death 6 (PDCD6) 10 ug
$450.00
RC202030L3 Lenti ORF clone of Human programmed cell death 6 (PDCD6), Myc-DDK-tagged 10 ug
$750.00
RC202030L4 Lenti ORF clone of Human programmed cell death 6 (PDCD6), mGFP tagged 10 ug
$750.00
RG202030 PDCD6 (tGFP-tagged) - Human programmed cell death 6 (PDCD6) 10 ug
$650.00

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