PDCD6 (NM_013232) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC202030L4
Lenti ORF clone of Human programmed cell death 6 (PDCD6), mGFP tagged
  $750.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDCD6
Synonyms ALG-2; ALG2; PEF1B
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC202030).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013232
ORF Size 573 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013232.2
RefSeq Size 1151 bp
RefSeq ORF 576 bp
Locus ID 10016
UniProt ID O75340
Cytogenetics 5p15.33
Domains EFh
Protein Families Druggable Genome
MW 21.9 kDa
Summary This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. provided by RefSeq, May 2012
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Documents
Resources
"NM_013232" in other vectors (4)
SKU Description Size Price
RC202030 PDCD6 (Myc-DDK-tagged)-Human programmed cell death 6 (PDCD6) 10 ug
$450.00
RC202030L3 Lenti ORF clone of Human programmed cell death 6 (PDCD6), Myc-DDK-tagged 10 ug
$750.00
RG202030 PDCD6 (tGFP-tagged) - Human programmed cell death 6 (PDCD6) 10 ug
$650.00
SC115334 PDCD6 (untagged)-Human programmed cell death 6 (PDCD6) 10 ug
$300.00

Plasmid Map for RC202030L4

Download sequence for

Cloning scheme for RC202030L4

Circular map for RC202030L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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