TMS1 (PYCARD) (NM_013258) Human Untagged Clone

SKU SC115313

PYCARD (untagged)-Human PYD and CARD domain containing (PYCARD), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMS1
• Synonyms: ASC; CARD5; TMS; TMS-1; TMS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_013258 edited; GAATTCGGCACGAGGCATGGGGCGCGCGCGCGACGCCATCCTGGATGCGCTGGAGAACCT; GACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCTGCTGTCGGTGCCGCTGCGCGAGGG; CTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCATGGACGCCTTGGACCTCACCGACAA; GCTGGTCAGCTTTTACCTGGAGACCTACGGCGCCGAGCTCACCGCTAACGTGCTGCGCGA; CATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGCGGCCACGCACCAGGGCTCTGGAGC; CGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGCAGCCAAGCCAGGCCTGCACTTTAT; AGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCACAAACGTTGAGTGGCTGCTGGATGC; TCTGTACGGGAAGGTCCTGACGGATGAGCAGTACCAGGCAGTGCGGGCCGAGCCCACCAA; CCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACCAGCCTGGAACTGGACCTGCAAGGA; CTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTACCTGGTGGAGGACCTGGAGCGGAG; CTGAGGCTCCTTCCCAGCAACACTCCGGTCAGCCCCTGGCAATCCCACCAAATCATCCTG; AATCTGATCTTTTTATACACAATATACGAAAAGCCAGCTTGAAAAAAAAAAAAAAAAAAC; TCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013258 unedited; TACGACTCTCTATAGGGCGGCCGCGAATTCGGCACGAGGCATGGGGCGCGCGCGCGACGC; CATCCTGGATGCGCTGGAGAACCTGACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCT; GCTGTCGGTGCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCAT; GGACGCCTTGGACCTCACCGACAAGCTGGTCAGCTTTTACCTGGAGACCTACGGCGCCGA; GCTCACCGCTAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGC; GGCCACGCACCAGGGCTCTGGAGCCGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGC; AGCCAAGCCAGGCCTGCACTTTATAGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCAC; AAACGTTGAGTGGCTGCTGGATGCTCTGTACGGGAAGGTCCTGACCGATGAGCAGTACCA; GGCAGTGCGGGCCGAGCCCACCAACCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACC; AGCCTGGAACTGCACCTGCAAAGACTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTA; CCTGGTGGAGGACCTGGAACGGAGCTGAAGCTTCTTCCCACACACACTTCCGTTAAGCCC; CTGCCAATCCCACCAAATAATCCTGAATCTGATCTTTTATTCACCTTTTACGAAAAGCCC; CTTTGAAAAAAAAAAAAAAAACTTCACCTTAATTGCGGCCCCGGGCATATTTTTTCCCTG; ACAAATCCCCGGGGGATCTCTGGAACCCTCCCAAAGCCTTTCCTGGGCCTGAAAATTGGA; CCTCCAGGGGCCCCCAACCTTGTCCTAAAAAATAAAATTGCCAATTTGGTCGCCACGGGG; GCCCCTTTAAAATATCGGGGGAAGGGGGGGGTTCTGACCCACGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013258 unedited; GTACCGCGGCNCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTCAAGCTGGCTTTTC; GTATATTGTGTATAAAAAGATCAGATTCAGGATGATTTGGTGGGATTGCCAGGGGCTGAC; CGGAGTGTTGCTGGGAAGGAGCCTCAGCTCCGCTCCAGGTCCTCCACCAGGTAGGACTGG; GACTCCCTTAGGGCCTGGAGGAGCAAGTCCTTGCAGGTCCAGTTCCAGGCTGGTGTGAAA; CTGAAAAGCTTCCGCATCTTGCTTGGGTTGGTGGGCTCGGCCCGCACTGCCTGGTACTGC; TCATCCGTCAGGACCTTCCCGTACAGAGCATCCACCAGCCACTCAACGTTTGTGACCCTC; CCGATAAGCGCACCCCGGTGCTGGTCTATAAAGCGCACGCCTGGCTTGGCTGCCGACTGA; GGAGGGGCCTGGATCCCACCTGGGGCGGCTTCACAGCCCTGGTGCCTGGCCGCCTGCCCC; TGCTCCGCCCTCTCCTGCAGGCCCCTTGCCCCCCATCGCCCTCCCGGTCACTTTCGCGCT; CATCGTCTCCCCCGTTCACCCCCTCCCTTTCTTCGCTAGCTCCCCCCTTTTCCTTCACCT; TCCCCCCTCCCCCATACCTCTTCTCCATCTCTCCGTCCTACCCGCCCTATTCTCCGCCCC; GATTCCTGATCTCCCTCCTTTCCCCGCTTTCTCGCCACCCTCCCCTTCTTCTTTCTCCCT; CCCCCCACCCCCCTCCCTGTCCCCACCCTCTTCAACCTCTCCCCCCCTTCTATCCCCCGC; ACCCCCCCCCTACGCCTATACGCCTCTCTAACACCCCCCCCGCTCTTCTCCCCCCCCCCC; GTTCTCCCCCCCCCCACCTCCTATTGCCTCGCCTGCCCTCCTGTTTTTTCCCTGCCGCCC; ACGTCTCTTCACCCCATCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_013258
• Insert Size: 730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013258.3, NP_037390.2
• RefSeq Size: 936 bp
• RefSeq ORF: 588 bp
• Locus ID: 29108
• UniProt ID: Q9ULZ3
• Cytogenetics: 16p11.2
• Domains: PAAD_DAPIN
• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
• Summary: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).