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TMS1 (PYCARD) (NM_013258) Human Untagged Clone

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SKU
SC115313
PYCARD (untagged)-Human PYD and CARD domain containing (PYCARD), transcript variant 1
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TMS1
Synonyms ASC; CARD5; TMS; TMS-1; TMS1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_013258 edited
GAATTCGGCACGAGGCATGGGGCGCGCGCGCGACGCCATCCTGGATGCGCTGGAGAACCT
GACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCTGCTGTCGGTGCCGCTGCGCGAGGG
CTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCATGGACGCCTTGGACCTCACCGACAA
GCTGGTCAGCTTTTACCTGGAGACCTACGGCGCCGAGCTCACCGCTAACGTGCTGCGCGA
CATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGCGGCCACGCACCAGGGCTCTGGAGC
CGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGCAGCCAAGCCAGGCCTGCACTTTAT
AGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCACAAACGTTGAGTGGCTGCTGGATGC
TCTGTACGGGAAGGTCCTGACGGATGAGCAGTACCAGGCAGTGCGGGCCGAGCCCACCAA
CCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACCAGCCTGGAACTGGACCTGCAAGGA
CTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTACCTGGTGGAGGACCTGGAGCGGAG
CTGAGGCTCCTTCCCAGCAACACTCCGGTCAGCCCCTGGCAATCCCACCAAATCATCCTG
AATCTGATCTTTTTATACACAATATACGAAAAGCCAGCTTGAAAAAAAAAAAAAAAAAAC
TCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_013258 unedited
TACGACTCTCTATAGGGCGGCCGCGAATTCGGCACGAGGCATGGGGCGCGCGCGCGACGC
CATCCTGGATGCGCTGGAGAACCTGACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCT
GCTGTCGGTGCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCAT
GGACGCCTTGGACCTCACCGACAAGCTGGTCAGCTTTTACCTGGAGACCTACGGCGCCGA
GCTCACCGCTAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGC
GGCCACGCACCAGGGCTCTGGAGCCGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGC
AGCCAAGCCAGGCCTGCACTTTATAGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCAC
AAACGTTGAGTGGCTGCTGGATGCTCTGTACGGGAAGGTCCTGACCGATGAGCAGTACCA
GGCAGTGCGGGCCGAGCCCACCAACCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACC
AGCCTGGAACTGCACCTGCAAAGACTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTA
CCTGGTGGAGGACCTGGAACGGAGCTGAAGCTTCTTCCCACACACACTTCCGTTAAGCCC
CTGCCAATCCCACCAAATAATCCTGAATCTGATCTTTTATTCACCTTTTACGAAAAGCCC
CTTTGAAAAAAAAAAAAAAAACTTCACCTTAATTGCGGCCCCGGGCATATTTTTTCCCTG
ACAAATCCCCGGGGGATCTCTGGAACCCTCCCAAAGCCTTTCCTGGGCCTGAAAATTGGA
CCTCCAGGGGCCCCCAACCTTGTCCTAAAAAATAAAATTGCCAATTTGGTCGCCACGGGG
GCCCCTTTAAAATATCGGGGGAAGGGGGGGGTTCTGACCCACGN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_013258 unedited
GTACCGCGGCNCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTCAAGCTGGCTTTTC
GTATATTGTGTATAAAAAGATCAGATTCAGGATGATTTGGTGGGATTGCCAGGGGCTGAC
CGGAGTGTTGCTGGGAAGGAGCCTCAGCTCCGCTCCAGGTCCTCCACCAGGTAGGACTGG
GACTCCCTTAGGGCCTGGAGGAGCAAGTCCTTGCAGGTCCAGTTCCAGGCTGGTGTGAAA
CTGAAAAGCTTCCGCATCTTGCTTGGGTTGGTGGGCTCGGCCCGCACTGCCTGGTACTGC
TCATCCGTCAGGACCTTCCCGTACAGAGCATCCACCAGCCACTCAACGTTTGTGACCCTC
CCGATAAGCGCACCCCGGTGCTGGTCTATAAAGCGCACGCCTGGCTTGGCTGCCGACTGA
GGAGGGGCCTGGATCCCACCTGGGGCGGCTTCACAGCCCTGGTGCCTGGCCGCCTGCCCC
TGCTCCGCCCTCTCCTGCAGGCCCCTTGCCCCCCATCGCCCTCCCGGTCACTTTCGCGCT
CATCGTCTCCCCCGTTCACCCCCTCCCTTTCTTCGCTAGCTCCCCCCTTTTCCTTCACCT
TCCCCCCTCCCCCATACCTCTTCTCCATCTCTCCGTCCTACCCGCCCTATTCTCCGCCCC
GATTCCTGATCTCCCTCCTTTCCCCGCTTTCTCGCCACCCTCCCCTTCTTCTTTCTCCCT
CCCCCCACCCCCCTCCCTGTCCCCACCCTCTTCAACCTCTCCCCCCCTTCTATCCCCCGC
ACCCCCCCCCTACGCCTATACGCCTCTCTAACACCCCCCCCGCTCTTCTCCCCCCCCCCC
GTTCTCCCCCCCCCCACCTCCTATTGCCTCGCCTGCCCTCCTGTTTTTTCCCTGCCGCCC
ACGTCTCTTCACCCCATCCC
Restriction Sites NotI-NotI |
ACCN NM_013258
Insert Size 730 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013258.3, NP_037390.2
RefSeq Size 936 bp
RefSeq ORF 588 bp
Locus ID 29108
UniProt ID Q9ULZ3
Cytogenetics 16p11.2
Domains PAAD_DAPIN
Protein Families Druggable Genome
Protein Pathways Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
Summary This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
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Documents
Resources
"NM_013258" in other vectors (6)
SKU Description Size Price
RC215592 PYCARD (Myc-DDK-tagged)-Human PYD and CARD domain containing (PYCARD), transcript variant 1 10 ug
$300.00
RC215592L1 Lenti ORF clone of Human PYD and CARD domain containing (PYCARD), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215592L2 Lenti ORF clone of Human PYD and CARD domain containing (PYCARD), transcript variant 1, mGFP tagged 10 ug
$600.00
RC215592L3 Lenti ORF clone of Human PYD and CARD domain containing (PYCARD), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215592L4 Lenti ORF clone of Human PYD and CARD domain containing (PYCARD), transcript variant 1, mGFP tagged 10 ug
$600.00
RG215592 PYCARD (tGFP-tagged) - Human PYD and CARD domain containing (PYCARD), transcript variant 1 10 ug
$489.00 $500.00

file-alt Citations

Gene-based vaccination and screening methods to develop monoclonal antibodies
Daftarian, Vosoughi, Lemmon
Methods Mol. Biol. (2014) 1121, 337-46 DOI: 10.1007/978-1-4614-9632-8_30
Cited by 3
Snippet: Dr. V. Lemmon, University of Miami), pcDNA3-vGPCR (G protein-coupled receptor) and pcDNA3 empty (provided by Dr. E. Mesri, University of Miami), pEGFP-C2-uPAR (provided by Dr. J. Reiser, University of Miami), pCMV6-XL5-PYCARD V1 (OriGene Technologies ... Cite SaveSaving
Inflammasome activation has an important role in the development of spontaneous colitis
Zhang, Fu, Sun et al
Mucosal Immunol (2014)
Cited by 125
AI Summary: In this study, OriGene’s ASC expression plasmid was used to reconstitute the inflammasome in 293T cells, enabling the authors to investigate the molecular mechanisms of inflammasome activation in chronic intestinal inflammation.
Snippet: ELISA. Inflammasome reconstitution in 293T cells. Briefly, 293T cells were transfected with expression plasmids for human NLRP3 (Invivogen), ASC (Origene, Rockville, MD), caspase-1 (Addgene, Cambridge, MA), and IL-1β. At ... Cite SaveSaving
5-azacytidine reverses drug resistance in bladder cancer cells
Ramachandran, Gordian, Singal
Anticancer Res. (2011) 31 (11), 3757-66
Cited by 5
AI Summary: OriGene’s pCMV6-XL5/TMS1 and pCMV6-XL5/Mock plasmids were used to transfect bladder cancer cells, enabling the study of TMS1’s role in reversing drug resistance.
Snippet: Transfection. UMUC3 cells were transfected with either pCMV6-XL5/TMS1 plasmid or pCMV6-XL5/Mock plasmid (Origene, Rockville, MD, USA). ... TMS1 was found to be down regulated by promoter methylation in the breast cancer cell line SKBR3.
Showing 1-3 of 3 papers.
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