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NCF4 (NM_013416) Human Untagged Clone

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SKU
SC115259
NCF4 (untagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NCF4
Synonyms CGD3; NCF; P40PHOX; SH3PXD4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_013416, the custom clone sequence may differ by one or more nucleotides


ATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGATGATGTTGCCATCTCGG
CCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTTTTCGTCATCGAGGTGAAGAC
AAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGCCAGTTCCATGCTTTGCAGAGCAAGCTGGAG
GAGCGCTTCGGGCCAGACAGCAAGAGCAGTGCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCT
ACGTGGGTGTGAAACAGGAGATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCT
CAGCCTGCCGGTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCA
GAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTGTCCCCACAGG
GCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGACTTCACTGGAAACAGCAAACT
GGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTCAGTCGGATCAACAAAGACTGGCTGGAGGGC
ACTGTCCGGGGAGCCACGGGCATCTTCCCTCTCTCCTTCGTGAAGATCCTCAAAGACTTCCCTGAGGAGG
ACGACCCCACCAACTGGCTGCGTTGCTACTACTACGAAGACACCATCAGCACCATCAAGTCTGTGGCCTG
GGAGGGAGGGGCCTGTCCAGCCTTCCTGCCATCCCTACGACCACTGCCCCTCACATCACCTTCTCATGGG
TCCCTCTCCCACTCCAAAGCCCCCAGTGGCTCCCAGATGAGCCACAATGCTGTAACAAGCCATCAACGTC
CAGGGTGGCCTGGCCAGCCTCATTCCCCTTTCCCCCACCCCACACCCCACTTCCAGCCTGATGCCTCCTT
ACTCCAGCCTGTCACCCCCTTAGGGACATCGCGGTGGAGGAAGATCTCAGCAGCACTCCCCTATTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_013416 unedited
CCCAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGGACTCCC
AGACCACAGCTGAGACGAGACGCAGGGTGGCTGGAGGAAGTGAGAGGTGAACTCAGCCTG
GGACTGGCTGGGCGAGACTCTCCACCTGCTCCCTGGGACCATCGCCCACCATGGCTGTGG
CCCAGCAGCTGCGGGCCGAGAGTACCTTTGAACAGCTTCCGGATGATGTTGCCATCTCGG
CCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTTTTCGTCATCG
AGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGCCAGTTCCATG
CTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAGAGCAGTGCCCTGGCCT
GTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAACAGGAGCTCGCCGAGA
TGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGCCTGCCGGTCTGGGTGC
TGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCAGAGCAGGTGC
CCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTGTCCCCACAGG
GCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGACTTCACTGGAA
ACAGCAAACTGGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTCAGTCCGATCA
ACAAAGACTGGCTGGAGGGCACTGTTCCGGGAGCCACGGGCATCTTCCCTCTCCCCTTCG
TGAAGACCTCAAAGACTTCCCCTGAGAGGACGACCCCACCACTGGCTGCGTTGCTCTACA
CGAAGACACATNGGACCATCAGGACATGCGTGGAGGAGTCTCCCAGCACTCCCTATGAAG
ACTGCTGGACTACAGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_013416 unedited
GGGGGGGCCGGGCCCGNGCCCCCTTCNNNNNGTNNGTACTTGNACGCGGCCGCTTTTNAN
NGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTCAAATGGATGGTTTATTTAAATCAGATG
TAAATAGTAGAAAGAAGAAGATTACAAGCCCCACGGGTCCATTAGCCTCAAAAACAGGAA
GTCTGCCCATCCTCCCAGGTTTTCCTGGTCCCAATCTCCTCTGAGAGCTGAAGGTTTTTG
CTGGTGTCCCCTCACTGCTCCAGGGACACCGTCAGCTCATGGCATCGTGTTGTAAACCCT
GTAGTTGTCCTTCTGCGTGATGTGCAGCTTCCAGGGGAAGAGGCGCTTCTGGGAGGGGAG
GCCACGAGCCTGCCGCACCATGAGCGCTACGTCCTCATCCGACAGCAGCCGAACCAGATC
CCCCTCAGCGTCCCGGTAATTCAGAGCTATGTCCTCTCTCTGGAACTCCCGCCTTGTGAG
CTCCAGCAGGTCTTTCAATAGGGGAGTGCTGCTGAGATCTTCCTCCACCGCGATGTCCTT
GATGGTGCTGATGGTGTCTTCGTAGTAGTAGCAACGCAGCCAGTTGGTGGGGTCGTCCTC
CTCAGGGAAGTCTTTGAGGATCTTCACGAAGGAGAGAGGGAAGATGCCCGTGGCTCCCCG
GACAGTGCCCTCCAGCCAGTCTTTGTTGATCCGACTGAGGAGGAAGATCACATTTCCAGC
TTTGAAATTCAGCTCCAGTTTGCTGTTTCCAGTGAAGCCAAATGAGCCTCTGCTCTCGGC
CTGCCATGCCGTCAACGCTGTTGCCCTGGGGGGACCCCTTTTTGACTTTCCCGGTGCCNC
GGCCGGAACCGGCCGAATGCCCTGGGGCACCTGCTTTGGATTATAAGGCGACTGGTAAAA
AAAGAACCGGACGTCCTCATCCTTAG
Restriction Sites NotI-NotI |
ACCN NM_013416
Insert Size 1470 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013416.2, NP_038202.1
RefSeq Size 1631 bp
RefSeq ORF 1047 bp
Locus ID 4689
UniProt ID Q15080
Cytogenetics 22q12.3
Domains PX, SH3
Protein Pathways Leukocyte transendothelial migration
Summary The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). This isoform (2) lacks the PC (Phox and Cdc24p) motif, which may be important for the interaction with NCF2.
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"NM_013416" in other vectors (6)
SKU Description Size Price
RC201191 NCF4 (Myc-DDK-tagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2 10 ug
$457.00
RC201191L1 Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC201191L2 Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, mGFP tagged 10 ug
$757.00
RC201191L3 Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC201191L4 Lenti ORF clone of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, mGFP tagged 10 ug
$757.00
RG201191 NCF4 (tGFP-tagged) - Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2 10 ug
$657.00

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