CHMP2B (NM_014043) Human Untagged Clone

SKU SC115190

CHMP2B (untagged)-Human chromatin modifying protein 2B (CHMP2B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CHMP2B
• Synonyms: ALS17; CHMP2.5; DMT1; FTDALS7; VPS2-2; VPS2B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014043, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGTCCCTCTTCAAGAAGAAAACCGTGGATGATGTAATAAAGGAACAGAATCGAGAGTTACGAGGTA; CACAGAGGGCTATAATCAGAGATCGAGCAGCTTTAGAGAAACAAGAAAAACAGCTGGAATTAGAAATTAA; GAAAATGGCCAAGATTGGTAATAAGGAAGCTTGCAAAGTTTTAGCCAAACAACTTGTGCATCTACGGAAA; CAGAAGACGAGAACTTTTGCTGTAAGTTCAAAAGTTACTTCTATGTCTACACAAACAAAAGTGATGAATT; CCCAAATGAAGATGGCTGGAGCAATGTCTACTACAGCAAAAACAATGCAGGCAGTTAACAAGAAGATGGA; TCCACAAAAGACATTACAAACAATGCAGAATTTCCAGAAGGAAAACATGAAAATGGAAATGACTGAAGAA; ATGATCAATGATACACTTGATGACATCTTTGACGGTTCTGATGACGAAGAAGAAAGCCAGGATATTGTGA; ATCAAGTTCTTGATGAAATTGGAATTGAAATTTCTGGAAAGATGGCCAAAGCTCCATCAGCTGCTCGAAG; CTTACCATCTGCCTCTACTTCAAAGGCTACAATCTCAGATGAAGAGATTGAACGGCAACTCAAGGCTTTA; GGAGTAGATTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014043 unedited; GTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTTCCCGGTC; ACCTGAGCTCCGGGTGACGCGGCTGCGGTAGCTGCGNGAACAAGCCTTCCGCGGGTCCTG; CCTGGCGACCCCGACCTCCTCCTGCTGTCTCTCCGCTCCGCCACCCCGAACCCGCCAAGG; TCCTGTCCTTTTCCTCCTGTCCTTTGCCAGCGTTGGGCCGGACCGGGCCGAGCCGGGCCG; CCCGGGCGCAGTCTTTAACCATGGCGTCCCTCTTCAAGAAGAAAACCGTGGATGATGTAA; TAAAGGAACAGAATCGAGAGTTACGAGGTACACAGAGGGCTATAATCAGAGATCGAGCAG; CTTTAGAGAAACAAGAAAAACAGCTGGAATTAGAAATTAAGAAAATGGCCAAGATTGGTA; ATAAGGAAGCTTGCAAAGTTTTAGCCAAACAACTTGTGCATCTACGGAAACAGAAGACGA; GAACTTTTGCTGTAAGTTCAAAAGTTACTTCTATGTCTACACAAACAAAAGTGATGAATT; CCCAAATGAAGATGGCTGGAGCAATGTCTACCACAGCAAAAACAATGCAGGCAGTTAACA; AGAAGATGGATCCACAAAAGACATTACAAACAATGCAGAATTTCCAGAAGGAAAACATGA; AAATGGAAATGACTGAAGAAATGATCAATGATACACTTGATGACATCTTTGACGGTTCTG; ATGACGAAGAAGAAAGCCAGGATATTGTGAATCAAGTTCTTGATGAAATTGGAATTNGAA; ATTCTGGAAAGATGCCAAAGCTCCATCAGCTGCTCGAAGCTTACCATCTGCCTCTACGTC; ANAGGGCTACATCTCAGATGAAGAGAATGAACGGCACTCAGGNNCTTTAGAGTAGTATAG; TCAAAAGAGTCATACTATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014043 unedited; AGCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCAAAAAC; ATGTGTTTTGTAAAAAAAATCTGCACTGTGCTTGGTTTATACTACATAATTATAAGTAAG; CAAAATAGTATGACTTCTTTTGACTAATCTACTCCTAAAGCCTTGAGTTGCCGTTCAATC; TCTTCATCTGAGATTGTAGCCTTTGACGTAGAGGCAGATGGTAAGCTTCGAGCAGCTGAT; GGAGCTTTGGCCATCTTTCCAGAAATTTCAATTCCAATTTCATCAAGAACTTGATTCACA; ATATCCTGGCTTTCTTCTTCGTCATCAGAACCGTCAAAGATGTCATCAAGTGTATCATTG; ATCATTTCTTCAGTCATTTCCATTTTCATGTTTTCCTTCTGGAAATTCTGCATTGTTTGT; AATGTCTTTTGTGGATCCATCTTCTTGTTAACTGCCTGCATTGTTTTTGCTGTGGTAGAC; ATTGCTCCAGCCATCTTCATTTGGGAATTCATCACTTTTGTTTGTGTAGACATAGAAGTA; ACTTTTGAACTTACAGCAAAAGTTCTCGTCTTCTGTTTCCGTAGATGCACAAGTTGTTTG; GCTAAAACTTTGCAAGCTTCCTTATTACCAATCTTGGCCATTTTCTTAATTTCTAATTCC; AGCTGTTTTTCTTGTTTCTCTAAAGCTGCTCGATCTCTGATTATAGCCCTCTGTGTACCT; CGTAACTCTCGATTCTGTTCCTTTATTACATCATCCACGGGTTTCTTCTTGAAGAGGGAC; GCCATGGTTAAAGACCTGCGCCGGGCGGCCCGGCTCGGNCCGGTCCCGCCCAACCGCTGC; AAGGGACAGAGAAAAGGACAGGACCTGNCGGGNTCGGGGTGCGNNNACGAAAACNNNCAG; AGAGTCGGGTCCCCAGCCAGACGCCGAAGGCTGTTTCCACTACCGANNCCGTACCCGAGC; TAGTGACGGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_014043
• Insert Size: 890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014043.2, NP_054762.2
• RefSeq Size: 2410 bp
• RefSeq ORF: 642 bp
• Locus ID: 25978
• UniProt ID: Q9UQN3
• Cytogenetics: 3p11.2
• Domains: DUF279
• Protein Pathways: Endocytosis
• Summary: This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).