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NTAQ1 (NM_018024) Human Untagged Clone

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SKU
SC113806
WDYHV1 (untagged)-Human WDYHV motif containing 1 (WDYHV1)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NTAQ1
Synonyms C8orf32; WDYHV1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113806 sequence for NM_018024 edited (data generated by NextGen Sequencing)
ATGGAAGGTAATGGCCCCGCTGCTGTCCACTACCAGCCGGCCAGCCCCCCGCGGGACGCC
TGCGTCTACAGCAGCTGCTACTGTGAAGAAAATGTTTGGAAGCTCTGTGAATACATCAAA
AACCATGACCAGTATCCTTTAGAAGAATGTTATGCTGTCTTCATATCTAATGAGAGGAAG
ATGATACCTATCTGGAAACAACAGGCGAGACCTGGAGATGGACCTGTGATCTGGGATTAC
CATGTTGTTTTGCTTCATGTTTCAAGTGGAGGACAGAGCTTCATTTATGATCTCGATACT
GTCTTGCCATTTCCCTGCCTCTTTGACACTTATGTAGAAGATGCCATTAAGTCTGATGAT
GACATTCACCCACAGTTTAGGAGGAAATTTAGAGTGATCTGTGCAGATTCATATTTGAAG
AACTTTGCTTCTGACCGATCTCACATGAAAGACTCCAGTGGGAATTGGAGAGAGCCTCCG
CCGCCATATCCCTGCATTGAGACTGGAGATTCCAAAATGAACCTGAACGATTTCATCAGT
ATGGATCCCAAGGTAGGATGGGGCGCCGTCTACACACTATCCGAATTTACACATCGGTTT
GGCAGTAAAAACTGCTGA

Clone variation with respect to NM_018024.1
94 a=>g;278 a=>g;346 t=>a;400 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018024 unedited
GGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCGGTCTT
CCTCCGGCCCGGGCCCTGGCCCAGCTAGCCGGCCATGGAAGGTAATGGCCCCGCTGCTGT
CCACTACCAGCCGGCCAGCCCCCCGCGGGACGCCTGCGTCTACAGCAGCTGCTACTGTGA
AGAAAATGTTTGGAAGCTCTGTGAATACATCAAAAACCATGACCAGTATCCTTTAGAAGA
ATGTTATGCTGTCTTCATATCTAATGAGAGGAAGATGATACCTATCTGGAAACAACAGGC
GAGACCTGGAGATGGACCTGTGATCTGGGATTACCATGTTGTTTTGCTTCATGTTTCAAG
TGGAGGACAGAGCTTCATTTATGATCTCGATACTGTCTTGCCATTTCCCTGCCTCTTTGA
CACTTATGTAGAAGATGCCATTAAGTCTGATGATGACATTCACCCACAGTTTAGGAGGAA
ATTTAGAGTGATCTGTGCAGATTCATATTTGAAGAACTTTGCTTCTGACCGATCTCACAT
GAAAGACTCCAGTGGGAATTGGAGAGAGCCTCCGCCGCCATATCCCTGCATTGAGACTGG
AGATTCCAAAATGAACCTGAACGATTTCATCAGTATGGATCCCAAGGTAGGATGGGGCGC
CGTCTACACACTATCCGAATTTACACATCCGGTTTGGCAGTAAAAACTGCTGAACTTGGC
CTCAAGATGTGGAACTGTGGGAGAATTCTAGGACATGAACCAGCTATCCTTTCATCCGAG
ACAGCANACATTATGGGTACAGTTTGGCTTGGAATTATTGCCTTCCTCTTTTAATTNTGA
TTGAGNGGGAAATCTGATGGAGTCCCAATTTTAATTGAACCACATTANAAACTTTTTGTT
NNTGACATGTCACATTGAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018024 unedited
NNAAAGTTTTTACTCTGAAACCGCGGCCGCATNCTAGNGATCAGTTTTTTTTTTTTTTTT
TTTTTTTCTGAGAGGAGTCTTGCTCTGTCACTCAGGCTGGAGTGCAGCAGCACGATCTCA
GCTCACTGCAACCTCCACCTCCCAGGTCCAAGCAATTCTGCCTCAGCCTCCCAAGTAGCT
GGCATCACAGGCGCCCGCCACCACACCCAGCCAACCTCTATATTTTTAGTAGAGACAAGG
TTTCACCACGTTGGCCAAGCTGGTCTCAAACTCCTGACCTCATGATCCGCCCACCTAGGA
CTCCCAAAGTGCTGAGATTACACACATGAGCCACCATGCCCAGCTGATAGTTTCTTAATA
AGTAGAAAGCTCATCTAAGAACAAAAGGTCAGACATCCTTGCTATAAGAAGTTATTCATT
TTTAAAGCAGAATCATGAAAACATGAGAGCTGGTACTATATGCCAATGTAATGCCAAATA
TCTTTCATTTGGAATGATATGATTAAAGCCAAATAACCAAGATGTCTTCCAGAAGGCACA
TGTAATAAATATTTTTTAAAGCCCTGGTTCTCATCTACAATTGAGGCATATTTAGGTATT
TCAGATCATCAAGGGTGACACAAAAATCTTCAAAACAAAACTAACGTTAAATCGATTTTA
TTTAAAGCCATAAATAAATAAGCCAATTAACGCTCAAGTCTGAGAGGGCTGCAGTCTTTT
TAACAATACCATAGTCCAAAAAGACTAATACTTATTGCTGATTCAGCTCACAATATTACC
CCTTTCCAGACAACAGCACATTCAAATGTTCAAGAAAACATTTTATGGGCACCTTTTATG
GGCATTTGAGATTACAGAGCAATGGGCCTGGCCCTGCCCCTCAGGACTTACGTGTACCTG
GAC
Restriction Sites NotI-NotI |
ACCN NM_018024
Insert Size 1900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018024.1, NP_060494.1
RefSeq Size 1348 bp
RefSeq ORF 618 bp
Locus ID 55093
UniProt ID Q96HA8
Cytogenetics 8q24.13
Protein Families Stem cell - Pluripotency
Summary Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_018024" in other vectors (6)
SKU Description Size Price
RC201055 WDYHV1 (Myc-DDK-tagged)-Human WDYHV motif containing 1 (WDYHV1) 10 ug
$300.00
RC201055L1 Lenti ORF clone of Human WDYHV motif containing 1 (WDYHV1), Myc-DDK-tagged 10 ug
$600.00
RC201055L2 Lenti ORF clone of Human WDYHV motif containing 1 (WDYHV1), mGFP tagged 10 ug
$600.00
RC201055L3 Lenti ORF clone of Human WDYHV motif containing 1 (WDYHV1), Myc-DDK-tagged 10 ug
$600.00
RC201055L4 Lenti ORF clone of Human WDYHV motif containing 1 (WDYHV1), mGFP tagged 10 ug
$600.00
RG201055 WDYHV1 (tGFP-tagged) - Human WDYHV motif containing 1 (WDYHV1) 10 ug
$489.00 $500.00

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