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PPP2R2D (NM_001003656) Human Untagged Clone

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SKU
SC113475
PPP2R2D (untagged)-Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2
  $300.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPP2R2D
Synonyms MDS026
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113475 sequence for NM_001003656 edited (data generated by NextGen Sequencing)
ATGGAGGAGCTGACCGAAGTCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTG
TTCGTCTACAGCAGTAGCAAAGGGACCATCCGCCTGTGTGACATGCGCTCCTCGGCCCTG
TGCGACAGACACTCCAAGTTTTTTGAAGAGCCTGAAGATCCCAGCAGTAGGTCCTTCTTC
TCAGAAATAATTTCATCCATATCCGATGTAAAATTCAGTCATAGTGGGCGGTACATGATG
ACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACATGGAGAGCAGGCCGGTGGAG
ACCCACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGAGAACGACTGC
ATCTTTGACAAGTTTGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCC
TATAACAACTTCTTCAGGATGTTTGATAGAGACACGCGGAGGGATGTGACCCTGGAGGCC
TCGAGAGAGAGCAGCAAACCGCGCGCCAGCCTCAAACCCCGGAAGGTGTGTACGGGGGGT
AAGCGGAGGAAAGACGAGATCAGTGTGGACAGTCTGGACTTCAACAAGAAGATCCTGCAC
ACAGCCTGGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATAACTTGTACATA
TTCCAGGACAAAATCAACTAG

Clone variation with respect to NM_001003656.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001003656 unedited
ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGAAAAGGGAAAAAAATCCC
TCCCCGGCGGCGGCGGCGGCGGCGGCGGCGCCGGCGGTGGTGGCGGCCCCGGGGCTGAGC
GCTCGGCTGCAGCGGCGCGGAGGCCGTCTCCCTGGTCTGCCGCGGTCCCCGCCCGTCCCG
CCGCCGGCTGCCATGGCAGGAGCCGGAGGCGGCGGCTGCCCCGCGGGCGGCAACGACTTC
CAGTGGTGCTTCTCGCAGGTCAAGGGGGCCATCGACGAGGACGTGGCCGAAGAATAAAAG
CCGCCCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGTCATGAACCGGA
GTTTGACTATTTGAAAAGTCTAGAAATTGAGGAAAAAATTAATAAAATTAGGTGGTTACC
ACAACAGAATGCTGCTCATTTTCTACTGTCTACAAATGATAAAACTATAAAATTATGGAA
AATAAGTGAACGGGATAAAAGAGCAGAAGGTTATAACCTGAAAGACGAAGATGGAAGACT
TCGAGACCCATTTAGGATCACGGCGCTACGGATGACCTGAGAATTAATTTATGGCACTTA
AAAATACAGATAGAAGCTTTAACATCGTGGACATCAAGCCTGCTAACATGGAGGAGCTGA
CCGAAGTCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACAGCA
GTAGCAAAGGGACCATCCGCCTGTGTGACATGCGCTCCTCGGCGCTGTGCGACAGACACT
CCAAGTTTTTTGAAGAGGCTGAGAATCCAGCAGTAGGTCCCTTCTTTCAAAAATAATCTA
TTCATATTCGATGTAAAATTAGTCCTATGGGCCGTTCATGATGACCACAAACTACCTGTG
GTGAAGGTGTGGGACCTCACAGGAAAGCAGGCCGGGGGACCCCCAGGCCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001003656 unedited
GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGATTTAAAAACACT
TTATAGTGTGTTGTTTTTATTGAGCGCTCACACCCGAAGGGGTGGCGGCGGATGCTGTGG
GTGAGTGGGGCCGCCTGAGCCTGCTCGGGCCACATCCACACATCCACAGAGTCCACCTGG
ACTCGGAGGAAGGCCGAGAGGACACGGACGGTGGCCACACCACGCTGCAGTGAAGGCCCA
AGTGTGATGGCAGAGAAAGAGGGAAAGTTGGAGAAAGAGCGGTATCTGACAATAACTTTT
CTCTTGGATGTTAATTTTTTGGTCTATAAATTGGAAAGGAAGGCTCGGACTGAAATAAAT
ACATTTATTCTGAGTAATGACCTTTTGGGAGCAGTGTCCGTCAACTCTGCTTCGAGAGCG
TCTCCACGTGAGCAGCAGCGCCTGTCTCGCGCCTCGCCGAGGCGGAACCACACCGGGCCT
CCAGCGGAGGCGGCTTTCTCCTGTGTCTGTGAAGGGAAGTAGCAGGTGCGTCACTGTTCT
TAATGGAGCGGACAATGATGCCTTTTCTCTGACAGAAAAATGTCCGGCTTAACTATGCAA
GACAAGACTTGGTCCTCACGTTCGCGTCTCTAGTTGATTNTGTCCTGGAATATGTACAAG
TTATTGGTGGCAGCCACGGCAATGACATTGTCCACGGGGTGCCANGCTGTGTGCAGGATC
TTCTTGTTGAAGTCCAGACTGTNCACACTGATCTCGTCTTTCCTCCGCTACCCCCCGTAC
ACACCTCCGGGGNTTGAGGCTGGCGCGCGGTTTGCTGCTCTCTCTTCGAGCCTCCANGGT
CACATCCCTCCGGGTGTNCTCATCAAACATNCTGNANAAATNTGTATAGGACCCCGTCAT
GATGGCGCTATTCGAACCGTTCAGCACACTCAACTGTCAAGATGCAGTCGTCT
Restriction Sites NotI-NotI |
ACCN NM_001003656
Insert Size 1960 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001003656.1, NP_001003656.1
RefSeq Size 2091 bp
RefSeq ORF 681 bp
Locus ID 55844
Cytogenetics 10q26.3
Protein Families Druggable Genome, Phosphatase
Protein Pathways Tight junction
Summary B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) has an additional exon in the 5' region and lacks an internal segment in the middle region, as compared to variant 1. It uses a downstream AUG start codon and encodes isoform 2 which has a shorter N-terminus, as compared to isoform 1.
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Documents
Resources
"NM_001003656" in other vectors (6)
SKU Description Size Price
RC221048 PPP2R2D (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2 10 ug
$300.00
RC221048L1 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC221048L2 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, mGFP tagged 10 ug
$600.00
RC221048L3 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC221048L4 Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2, mGFP tagged 10 ug
$600.00
RG221048 PPP2R2D (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R2D), transcript variant 2 10 ug
$489.00 $500.00

file-alt Citations

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