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AP1AR (NM_018569) Human Untagged Clone

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SKU
SC113430
AP1AR (untagged)-Human adaptor-related protein complex 1 associated regulatory protein (AP1AR), transcript variant 1
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol AP1AR
Synonyms 2C18; C4orf16; gamma-BAR; GBAR; PRO0971
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113430 sequence for NM_018569 edited (data generated by NextGen Sequencing)
ATGGGGAACTGCTGCTGGACGCAGTGCTTCGGACTGCTTCGCAAGGAAGCGGGGCGGCTG
CAGCGAGTAGGCGGCGGCGGAGGATCCAAGTATTTTAGAACATGCTCAAGAGGTGAGCAC
TTAACAATAGAGTTTGAGAATCTAGTAGAAAGTGATGAAGGGGAGAGCCCAGGAAGCAGT
CATAGGCCTCTTACTGAGGAAGAAATTGTTGACCTAAGAGAAAGGCATTATGATTCCATT
GCCGAAAAACAAAAAGATCTTGATAAGAAAATTCAAAAAGAGTTAGCCTTACAAGAAGAG
AAGTTAAGACTAGAAGAAGAAGCTTTATACGCTGCACAGCGTGAAGCAGCCAGGGCAGCA
AAGCAGCGAAAGCTCTTGGAGCAAGAAAGGCAGAGAATTGTGCAGCAATATCATCCTTCC
AACAATGGAGAATATCAAAGTTCAGGACCAGAAGATGACTTCGAATCTTGTTTGAGAAAT
ATGAAGTCACAGTATGAAGTTTTTCGAAGTAGTAGACTCTCATCAGATGCTACAGTTTTG
ACACCAAATACAGAAAGCAGTTGTGATTTAATGACCAAAACTAAATCAACTAGTGGAAAT
GACGACAGCACATCCTTAGATCTAGAGTGGGAAGATGAAGAAGGAATGAATAGAATGCTT
CCAATGAGAGAACGTTCCAAAACAGAGGAAGACATTCTACGGGCAGCACTTAAGTATAGC
AACAAGAAGACTGGAAGTAATCCTACATCAGCCTCTGATGATTCCAATGGGCTGGAGTGG
GAAAATGATTTTGTTAGTGCCGAAATGGATGATAATGGAAATTCCGAGTATTCTGGATTT
GTAAATCCTGTATTAGAACTGTCTGATTCTGGCATAAGGCATTCTGACACAGATCAACAG
ACTCGATAG

Clone variation with respect to NM_018569.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018569 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGAGGAGGAGCGGC
GGCGCCTGGGCGGCATGCGATGGGGAACTGCTGCTGGACGCAGTGCTTCGGACTGCTTCG
CAAGGAAGCGGGGCGGCTGCAGCGAGTAGGCGGCGGCGGAGGATCCAAGTATTTTAGAAC
ATGCTCAAGAGGTGAGCACTTAACAATAGAGTTTGAGAATCTAGTAGAAAGTGATGAAGG
GGAGAGCCCAGGAAGCAGTCATAGGCCTCTTACTGAGGAAGAAATTGTTGACCTAAGAGA
AAGGCATTATGATTCCATTGCCGAAAAACAAAAAGATCTTGATAAGAAAATTCAAAAAGA
GTTAGCCTTACAAGAAGAGAAGTTAAGACTAGAAGAAGAAGCTTTATACGCTGCACAGCG
TGAAGCAGCCAGGGCAGCAAAGCAGCGAAAGCTCTTGGAGCAAGAAAGGCAGAGAATTGT
GCAGCAATATCATCCTTCCAACAATGGAGAATATCAAAGTTCAGGACCAGAAGATGACTT
CGAATCTTGTTTGAGAAATATGAAGTCACAGTATGAAGTTTTTCGAAGTAGTAGACTCTC
ATCAGATGCTACAGTTTTGACACCAAATACAGAAAGCAGTTGTGATTTAATGACCAAAAC
TAAATCAACTAGTGGAAATGACGACAGCACATCCTTAGATCTAGAGTGGGAAGATGAAGA
AGGATGAATAGAATGCTTCCATGAGAGAACGTTCCANACAGAGGAAGACATTCTACGGGC
AGCACTTAAGTATAGCACAAGAGACTGGAAGTAATCTACATCACCTCTGATGATTCCATG
GNCTGAGTGGGAAATGATTTTGTTATGCCGAATGGTGATATGGAAATCCGAGANCTGGAT
TGTAAATCTGATTANAACTGTCTGATCTGGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018569 unedited
GCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGTGTAAAAATGTGTTTATTC
ACAACCATTTGTAAATTACCATTTGTTACCATTCCTTCAAAAAATTCTTTATGAACAAAA
AGCCAAGAAAAATTACAATCGTAGCTTATGCCAAACCAAAAATATACATCTGATGTATAC
AAACTTTAAAAAGCATTAACACAGGCAATGTGAATATAGATTACCTTAAAAATATTTCCA
AGACACAGCTACAACTTAAATTATGTTAAGACAGATATAAATAATAGCATTACCTAATGT
ATTATATTTACATATTTAAAGTATCATATTAATTTCTATGACATAAAAAAGGAGATAAAA
CTACAGATGTCATTGCTTAGCAGTTCAAATATTGAAAGGGCCACATGATTTTTTTTTTTT
AAGCACAGCAAGAACTTTAAGTTACTATTTATATCTGGACCTTCCTATATTTATAAGAAC
ATGCACCGTCATTGTTGCAAGAGGAAAAAAAATTAAAAACATTAAGGAAAAACCAAAAAA
CAGTTTGCTTACTAAAATCATACCAAATCCTCAGCAAAATCAAGGAAAGCAATTATGGGA
GTGTTTTCTATCCCTGGCCTTGTAAATTGACATATACATTTTAGAGAAAAGAACAGTAAT
TGTCTAGCACAAAATGCAGTTATAGATATTGATATGCTTAGTTCTATCCCATTAAAGCAC
CTTGTACTACAAGGTAAAGGGAAGGCTTAATAAAATCAATGGGAGCCTTTCATACCAGAA
AGGATTTTATTTGAAATACCCGTATTACCTTNNTAGAATTTCTTTCTGAAATATGCANAA
ATATGTTCTGTGTAAATCTACATCGTTATCGAATATAGAATTTTAAAATAATGCACTAAA
CTATACTGATTCANTAAAGCATTAGGGCTGAAATGAGTGGAAAACTATTTTTATATAACT
CTAAAC
Restriction Sites NotI-NotI |
ACCN NM_018569
Insert Size 2550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018569.2, NP_061039.2
RefSeq Size 2835 bp
RefSeq ORF 909 bp
Locus ID 55435
UniProt ID Q63HQ0
Cytogenetics 4q25
Summary Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_018569" in other vectors (4)
SKU Description Size Price
RC222729 AP1AR (Myc-DDK-tagged)-Human adaptor-related protein complex 1 associated regulatory protein (AP1AR), transcript variant 1 10 ug
$300.00
RC222729L3 Lenti ORF clone of Human adaptor-related protein complex 1 associated regulatory protein (AP1AR), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC222729L4 Lenti ORF clone of Human adaptor-related protein complex 1 associated regulatory protein (AP1AR), transcript variant 1, mGFP tagged 10 ug
$600.00
RG222729 AP1AR (tGFP-tagged) - Human adaptor-related protein complex 1 associated regulatory protein (AP1AR), transcript variant 1 10 ug
$500.00

file-alt Citations

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