TFB2M (NM_022366) Human Untagged Clone

SKU SC112537

TFB2M (untagged)-Human transcription factor B2, mitochondrial (TFB2M), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: TFB2M
• Synonyms: Hkp1; mtTFB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112537 sequence for NM_022366 edited (data generated by NextGen Sequencing); ATGTGGATCCCAGTGGTCGGGCTTCCTCGGCGGCTGAGGCTCTCCGCCTTGGCGGGCGCT; GGTCGCTTTTGCATTTTAGGGTCTGAAGCGGCGACGCGAAAGCATTTGCCGGCGAGGAAC; CACTGTGGGCTCTCTGACTCCTCTCCGCAGCTGTGGCCCGAACCGGATTTCAGGAATCCG; CCAAGGAAGGCGTCTAAGGCCAGCTTAGACTTTAAGCGTTACGTAACCGATCGGAGATTG; GCTGAGACCCTGGCGCAAATCTATTTGGGAAAACCAAGTAGACCTCCACACCTACTGCTG; GAGTGCAATCCAGGTCCTGGAATCCTGACTCAGGCATTACTTGAAGCTGGTGCCAAAGTG; GTTGCGCTCGAAAGTGACAAAACTTTTATTCCACATTTGGAGTCCTTAGGAAAAAATCTG; GATGGAAAACTACGAGTGATTCACTGTGACTTCTTTAAACTAGATCCTAGAAGTGGTGGA; GTAATAAAACCACCTGCTATGTCTTCTCGAGGGCTCTTTAAGAATTTGGGAATAGAAGCA; GTTCCTTGGACAGCAGACATCCCTTTAAAAGTAGTTGGAATGTTCCCAAGTAGAGGTGAG; AAAAGGGCACTTTGGAAACTCGCATATGACTTGTATTCCTGTACTTCTATATATAAATTT; GGACGAATAGAAGTAAATATGTTTATTGGTGAAAAAGAATTCCAGAAACTAATGGCAGAT; CCCGGAAATCCAGACTTGTATCATGTATTAAGTGTTATCTGGCAATTAGCTTGTGAGATT; AAGGTTCTGCACATGGAGCCTTGGTCATCATTTGATATATACACCCGGAAAGGGCCGCTG; GAAAACCCAAAGCGTAGGGAATTATTAGACCAATTACAACAAAAGCTGTATCTTATTCAA; ATGATTCCTCGTCAAAATTTATTTACCAAGAACTTAACACCTATGAACTATAATATATTT; TTTCACTTGTTAAAGCACTGTTTCGGGAGGCGCAGCGCCACTGTAATAGACCACTTACGT; TCATTGACTCCACTTGATGCGAGAGATATATTGATGCAAATAGGAAAACAGGAGGATGAG; AAAGTAGTTAACATGCACCCTCAAGACTTCAAAACACTTTTTGAAACTATAGAGCGTTCC; AAAGATTGTGCTTATAAATGGCTGTATGATGAAACCCTGGAAGATAGGTAG; ; Clone variation with respect to NM_022366.2; 984 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022366 unedited; GCGATTGTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGTGGGATACCCTGC; GTGGGGTTCACTGTAGTAGCTGCACTAGGTGATTCTTGGAGCGGGCCTGAGAGACAAGGA; CATGTGGATCCCAGTGGTCGGGCTTCCTCGGCGGCTGAGGCTCTCCGCCTTGGCGGGCGC; TGGTCGCTTTTGCATTTTAGGGTCTGAAGCGGCGACGCGAAAGCATTTGCCGGCGAGGAA; CCACTGTGGGCTCTCTGACTCCTCTCCGCAGCTGTGGCCCGAACCGGATTTCAGGAATCC; GCCAAGGAAGGCGTCTAAGGCCAGCTTAGACTTTAAGCGTTACGTAACCGATCGGAGATT; GGCTGAGACCCTGGCGCAAATCTATTTGGGAAAACCAAGTAGACCTCCACACCTACTGCT; GGAGTGCAATCCAGGTCCTGGAATCCTGACTCAGGCATTACTTGAAGCTGGTGCCAAAGT; GGTTGCGCTCGAAAGTGACAAAACTTTTATTCCACATTTGGAGTCCTTAGGAAAAAATCT; GGATGGAAAACTACGAGTGATTCACTGTGACTTCTTTAAACTAGATCCTAGAAGTGGTGG; AGTAATAAAACCACCTGCTATGTCTTCTCGAGGGCTCTTTAAGAATTTGGGAATAGAAGC; AGTTCCTTGGACAGCAGACATCCCTTTAAAAGTAGTTGGAATGTTCCCAAGTAGAGGTGA; GAAAAGGGCACTNTGGAAACTCGCATATGACTTGTATTCCTGTACTTCTATATATAAATT; TGGACGAATAGAAGTAAATATGTTTATTGGTGAAAAAGATTCCAGAAACTAATGGCAGAT; CCCCGGAATCCAGACCTGNATCATGTATTAAGTGGTATCTGGGCAANTAGCTGTGAGAAT; AAAGGTCTGCACATGGNACCCTNGNTCATCACTTGATATATACACCCGNNAAGGGCCGCT; GGAAACCCNAAGCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022366 unedited; GAACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTGAAGTTTTCA; TTTTATTTAATTGTGAATAAAATAGCAGACACTGTTTCATCCAATAAGCCAATGATATCA; GCTTAGGAGAAATGATCTGCCTGGCTTGTGCAAGACAAGAACAGTTACCTTCTGCTGAAA; GGATGTGAGTTTTCAAATTTGGTTTTCATGTCATAGTTTCCAAATAAATGAACCGCTCCA; CCAAAAACGACAGTCTAGTTGCTACCTATCTTCCAGGGTTTCATCATACAGCCATTTATA; AGCACAATCTTTGGAACGCTCTATAGTTTCAAAAAGTGTTTTGAAGTCTTGAGGGTGCAT; GTTAACTACTTTCTCATCCTCCTGTTTTCCTATTTGCATCAATATATCTCTCGCATCAAG; TGGAGTCAATGAACGTAAGTGGTCTATTACAGTGGCGCTGCGCCTCCCGAAACAGTGCTT; TAACAAGTGAAAAAATATATTATAGTTCATAGGTGTTAAGTTCTTGGTAAATAAATTTTG; ACGAGGAATCATTTGAATAAGATACAGCTTTTGTTGTAATTGGTCTAATAATTCCCTACG; CTTTGGGTTTTCCAGCGGCCCTTTCCGGGTGTATATATCAAATGATGACCAAGGCTCCAT; GTGCAGACCCTTAATCTCACAAGCTAATTGCCAGATAACACTTAATACATGATACAAGTC; TGGATTTCCGGGATCTGCCTTAATTTCCTGAATTCTTTTTCACCAATAAACATATTTACT; TCTATTCGTCCAATTTATTATAGAAGTCAGGAATCCCAGTCATATGCCAGTTTCCAAGCG; CCCTTTTTCACCTTACTTGGGACCATCCCACTACTTTTAAGGNATGTTTGCTGCCCAAGT; ACTGCTTTATTCCCAATTCTAAGAGCCTCGAAACACCTACAGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_022366
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022366.1, NP_071761.1
• RefSeq Size: 1803 bp
• RefSeq ORF: 1191 bp
• Locus ID: 64216
• UniProt ID: Q9H5Q4
• Cytogenetics: 1q44
• Domains: rADc
• Protein Families: Transcription Factors
• Summary: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.[UniProtKB/Swiss-Prot Function]