CENPH (NM_022909) Human Untagged Clone

SKU SC112402

CENPH (untagged)-Human centromere protein H (CENPH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CENPH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112402 sequence for NM_022909 edited (data generated by NextGen Sequencing); ATGGAGGAGCAGCCCCAGATGCAAGACGCCGACGAGCCCGCGGACTCCGGAGGGGAAGGC; CGGGCAGGCGGGCCACCGCAGGTCGCCGGCGCCCAGGCGGCGTGCAGCGAGGACCGCATG; ACCCTGCTCCTCAGGCTGAGAGCACAGACAAAACAACAACTCTTAGAATATAAATCAATG; GTTGATGCAAGTGAAGAAAAAACTCCAGAACAAATTATGCAAGAAAAGCAAATCGAAGCT; AAAATTGAAGACCTGGAAAATGAAATTGAAGAGGTAAAAGTTGCTTTTGAGATAAAAAAG; CTTGCATTAGACAGGATGAGACTTTCAACTGCACTTAAAAAAAACCTGGAGAAAATTAGC; AGACAGTCTAGTGTGCTCATGGATAACATGAAACACCTATTAGAGCTAAATAAATTAATA; ATGAAATCACAGCAGGAATCTTGGGATTTAGAGGAAAAACTGCTTGATATTAGAAAGAAG; AGATTGCAATTAAAACAAGCTTCAGAAAGTAAGCTTTTAGAAATACAGACTGAAAAGAAC; AAACAGAAGATTGATTTGGACAGTATGGAAAACTCAGAGAGGATAAAGATCATACGACAA; AACCTACAGATGGAGATAAAAATTACTACTGTTATTCAACATGTGTTCCAGAACCTTATT; TTGGGGAGTAAAGTCAATTGGGCAGAGGATCCTGCCCTTAAGGAAATTGTTCTGCAGCTT; GAGAAGAATGTTGACATGATGTAA; ; Clone variation with respect to NM_022909.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022909 unedited; GTATACGACTTTTATGGGCGGCCGCGAATTCGGCACCAGGCCTGTTGAGTGTTTGCCTTT; CCCCTCAACCAGCAATGGAGGAGCAGCCCCAGATGCAAGACGCCGACGAGCCCGCGGACT; CCGGAGGGGAAGGCCGGGCAGGCGGGCCACCGCAGGTCGCCGGCGCCCAGGCGGCGTGCA; GCGAGGACCGCATGACCCTGCTCCTCAGGCTGAGAGCACAGACAAAACAACAACTCTTAG; AATATAAATCAATGGTTGATGCAAGTGAAGAAAAAACTCCAGAACAAATTATGCAAGAAA; AGCAAATCGAAGCTAAAATTGAAGACCTGGAAAATGAAATTGAAGAGGTAAAAGTTGCTT; TTGAGATAAAAAAGCTTGCATTAGACAGGATGAGACTTTCAACTGCACTTAAAAAAAACC; TGGAGAAAATTAGCAGACAGTCTAGTGTGCTCATGGATAACATGAAACACCTATTAGAGC; TAAATAAATTAATAATGAAATCACAGCAGGAATCTTGGGATTTAGAGGAAAAACTGCTTG; ATATTAGAAAGAAAGAGATTGCATTAAAACAAGCTTCAGAAAGTAAGCTTTTAGAAATAC; AGACTGAAAAGAACANACAGAAGATTGATTTGGACAGTATGGAANACTCAGAGAGGGATA; AGATCATACGACANNACCTACAGATGGAGATAAAATTACTACTGTTATTCAACATGTGTT; CCAGAACCTTTATTTGGNNGAGTAAGTCANTTGGGCAGAGGATCCCTGCCCTTAAGGAAA; TTGNTCTGCAGCTTGAGAAAGATGGTGACATGATGTANNTAGAANTCATTTCTGACANNT; ATTACATTNCTGGGCATCTCAACTCTTATTTGGGATACTTNCTGTGCATTTGNCTGCCCA; CCGTTATTTTAGAAAAGCTATNCCATACCGTTTCAGTTGGTAGTACAGTTGTGGGTTAGT; ATTTNGCAGGGGGATNGAANGNNNATTTTTCTTTTTAATNNCTAAATTAAGNTGGNTTTT; GGGTGCGGCTGGTTTTGAGAGGAGGCCGCTTTGTGCCAGATGCNAGCAGTGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022909 unedited; GGGGCACAGACCCAACCTATANNNNNNNGNGGTCGTGNACCGCGCCGCATTTTANGATCG; GTTTTTTTTTTTTTTTTTTTTTTAACATTAATTCCTTTTATTTCTTAACTTATAAAACTG; ATAGAAAAAAATGCAAACTAGACCAGGCTCGGTGGCTTACGTCTGTAATCCTAGCACTTC; GGTAGGCCGAGGCGGTGGACTGCCTGAGCTCAGGAGTTCTGAGAGCAGCCTGGGCAACAC; GGTGAAACCCCGTCTCTACTAAAATACAAAAAATTAGCTGGGCATGGCGGCGTGCACCTT; TAGTCACAGCTACTTGGGAGGCTGAGGCAGAACTGCTTGAACCCGGGAGGCAGAGGTTGC; AGTGAGCCGAGATCGCGCCACTGCCCTCCAGTCTGGGCAACAAAGCGAGACTCCATCTCA; AAAAACAACAACAACAAAAAAACAAACTAAATATAGAAATATAAAAAGAAAAAAATTACT; TTCAATCCCACTACAAATAACTAACCACAACTGTACTACAACTGTAAACGTTATGGATAT; GCTTTTCTAAAATTACGGTGGACAGACAAATGCACAGAAGTATTCCAAATAAGAGTTGAG; ATTGCCAGAAATGTAAATATGTCAGAAATGAATTCTTATACATCATGTCAACATTCTTTT; CAAGCTGCAGAACAATTTCCTAAGGGCANGATCCCTCTGCCCATTGACTTTTACTCCCCC; AAATAAAGTTTCTGGAACACATTGTTGAATAACAGGAGTAAATTTTTATCTCCATCTGAA; GGTTTTGGCCAATGAACTTTATCCCCTCTGGAGTTTCCATACTTGCCAAAAAAAACTTCT; GGTTGTTCTTTCAGCNCCAATTCCTAAAGCCAACTTTTTGAAACCGGGTTAAATGCAACC; CTTTTTTTTAAATTAAGCAGATTTTCCTTTAAACCCAAAATCCGGCGGGAATTCATTATA; ATTAATTAACCCCAAAAGGGGTTCATGTTTCCCTGGACCCCCTCAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_022909
• Insert Size: 980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022909.3, NP_075060.1
• RefSeq Size: 1405 bp
• RefSeq ORF: 744 bp
• Locus ID: 64946
• UniProt ID: Q9H3R5
• Cytogenetics: 5q13.2
• Protein Families: Druggable Genome
• Summary: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]