RIC3 (NM_024557) Human Untagged Clone
SKU
SC112180
RIC3 (untagged)-Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | RIC3 |
| Synonyms | AYST720; PRO1385 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_024557 |
| Insert Size | 1107 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_024557.2, NP_078833.2 |
| RefSeq Size | 2907 bp |
| RefSeq ORF | 1107 bp |
| Locus ID | 79608 |
| UniProt ID | Q7Z5B4 |
| Cytogenetics | 11p15.4 |
| Protein Families | Transmembrane |
| Summary | This gene encodes a member of the resistance to inhibitors of cholinesterase 3-like family which functions as a chaperone of specific 5-hydroxytryptamine type 3 receptor and nicotinic acetylcholine receptor subtypes. The encoded protein influences the folding and assembly of these receptor subunits in the endoplasmic reticulum and expression on the cell surface. This protein contains an N-terminal transmembrane domain, a proline-rich spacer, and a cytosolic C-terminal coiled-coil domain. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2016 Transcript Variant: This variant (1) encodes isoform c. The isoform designation was changed from 'a' to 'c' to be consistent with isoforms cited in PMID 18691158. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_024557" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205179 | RIC3 (Myc-DDK-tagged)-Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1 | 10 ug |
$457.00
|
|
| RC205179L1 | Lenti ORF clone of Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC205179L2 | Lenti ORF clone of Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC205179L3 | Lenti ORF clone of Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC205179L4 | Lenti ORF clone of Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG205179 | RIC3 (tGFP-tagged) - Human resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (RIC3), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
Citations
NACHO Engages N-Glycosylation ER Chaperone Pathways for α7 Nicotinic Receptor Assembly
Kweon, Gu, Witham et al
Cell Rep (2020) 32 (6), 108025 DOI: 10.1016/j.celrep.2020.108025
Kweon, Gu, Witham et al
Cell Rep (2020) 32 (6), 108025 DOI: 10.1016/j.celrep.2020.108025
Cited by 23
AI Summary: OriGene products, specifically the human CHRNA7 Myc-DDK tagged cDNA (Cat# RC211456) and RIC3 Myc-DDK tagged cDNA (Cat# RC219445), were used in this study to express and analyze the roles of α7 nicotinic receptor and RIC-3 protein, enabling the investigation of NACHO-mediated receptor assembly and trafficking.
Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?
Deshpande, Vinayakamoorthy, Garg et al
Biomolecules (2020) 10 (3) DOI: 10.3390/biom10030470
Deshpande, Vinayakamoorthy, Garg et al
Biomolecules (2020) 10 (3) DOI: 10.3390/biom10030470
Cited by 17
AI Summary: In this study, the mouse alpha7 nicotinic acetylcholine receptor plasmid in pCMV6 with a DDK (FLAG) tag (OriGene catalog MR224522) was used to investigate the roles of NACHO and RIC3 in α7nAChR expression.
Snippet: While addressing these specific objectives, our findings generate new questions about the interactions between NACHO and RIC3. 2. …Mouse alpha7 in pCMV6 with a DDK (FLAG) tag was purchased from Origene (catalog MR224522 Cached;- While addressing these specific objectives, our findings generate new questions about the interactions between NACHO and RIC3. 2. …Mouse alpha7 in pCMV6 with a DDK (FLAG) tag was purchased from Origene (catalog MR224522 Cached
NACHO Engages N-Glycosylation ER Chaperone Pathways for α7 Nicotinic Receptor Assembly
Kweon, Gu, Witham et al
Cell Rep (2020) 32 (6), 108025 DOI: 10.1016/j.celrep.2020.108025
Kweon, Gu, Witham et al
Cell Rep (2020) 32 (6), 108025 DOI: 10.1016/j.celrep.2020.108025
Cited by 23
AI Summary: OriGene products, including mouse and human CHRNA7 (Cat# MC216944, SC124074), mouse and human HTR3A (Cat# MC216801, SC122578), human CHRNA3 (Cat# SC122160), human CHRNA9 (Cat# SC313657), human TMEM35A (Cat# SC112910), and human RIC3 (Cat# SC112180), provided essential plasmids for the expression and functional analysis of nicotinic receptor subunits and related proteins in this study.
Snippet: Mouse CHRNA7 (NM_007390.3) Origene Technologies Cat# MC216944 Human CHRNA7 (NM_000746.5) Origene Technologies Cat# SC124074 Mouse HTR3A (NM_013561.2) Origene Technologies Cat# MC216801 Human HTR3A (NM_000869.5) Origene Technologies Cat# SC122578 Human CHRNA3 (NM_000743.4) Origene Technologies Cat# SC122160 Human CHRNA9 (NM_017581.3) Origene Technologies Cat# SC313657 Human TMEM35A (NM_021637.2) Origene Technologies Cat# SC112910 Human RIC3 (NM_024557.5) Origene Technologies Cat# SC112180 Human CANX (NM_001746.3) Genecopoeia Cat# EX-K4904-M02
Oligoarginine Peptides, a New Family of Nicotinic Acetylcholine Receptor Inhibitors
Lebedev, Kryukova, Ivanov et al
Mol. Pharmacol. (2019) 96 (5), 664-673 DOI: 10.1124/mol.119.117713
Lebedev, Kryukova, Ivanov et al
Mol. Pharmacol. (2019) 96 (5), 664-673 DOI: 10.1124/mol.119.117713
AI Summary: In this study, the Ric3-pCMV6-XL5 plasmid from OriGene was used to transiently transfect Neuro2a cells, enabling the functional expression and analysis of nicotinic acetylcholine receptors for investigating the inhibitory effects of oligoarginine peptides.
Snippet: Neuro2a cells were transiently transfected with plasmids α7 nAChR-pCEP4, Ric3- pCMV6-XL5 (OriGene, Rockville, MD, USA) and pCase12-cyto vector (Evrogen, Moscow, Russia) utilizing lipofectamine transfection protocol (Invitrogen, Waltham, MA, USA) View as HTML;Neuro2a cells were transiently transfected with plasmids α7 nAChR-pCEP4, Ric3- pCMV6-XL5 (OriGene, Rockville, MD, USA) and pCase12-cyto vector (Evrogen, Moscow, Russia) utilizing lipofectamine transfection protocol (Invitrogen, Waltham, MA, USA) View as HTML
Curare alkaloids from Matis Dart Poison: Comparison with d-tubocurarine in interactions with nicotinic, 5-HT3 serotonin and GABAA receptors
Spirova, Ivanov, Kasheverov et al
PLoS ONE (2019) 14 (1), e0210182 DOI: 10.1371/journal.pone.0210182
Spirova, Ivanov, Kasheverov et al
PLoS ONE (2019) 14 (1), e0210182 DOI: 10.1371/journal.pone.0210182
AI Summary: OriGene products contributed to this study by providing the Ric3-pCMV6-XL5 and NACHO TMEM35-pCMV6-XL5 plasmid constructs used for the transient co-transfection and functional expression of human α7 nicotinic acetylcholine receptors in Neuro2a cells.
Snippet: followed by lipofectamine (Invitrogen, USA)-mediated transient co-transfection of human α7 nAChR-pCEP4, fluorescent calcium sensor pCase12-cyto (Evrogen, Russia) and chaperone Ric3-pCMV6-XL5 or NACHO TMEM35-pCMV6-XL5 plasmid constructs (OriGene Cached
Azemiopsin, a Selective Peptide Antagonist of Muscle Nicotinic Acetylcholine Receptor: Preclinical Evaluation as a Local Muscle Relaxant
Shelukhina, Zhmak, Lobanov et al
Toxins (Basel) (2018) 10 (1) DOI: 10.3390/toxins10010034
Shelukhina, Zhmak, Lobanov et al
Toxins (Basel) (2018) 10 (1) DOI: 10.3390/toxins10010034
AI Summary: OriGene’s Ric3-pCMV6-XL5 plasmid was used to co-express the human chaperone Ric-3 alongside human α7 nAChR in Neuro2a cells, enabling functional receptor expression for calcium imaging assays in the preclinical evaluation of azemiopsin as a muscle relaxant.
Snippet: …On the next day Neuro2a cells were transiently transfected with plasmids coding mouse muscle α1β1δε nAChR (pRBG4-vector) and a fluorescent calcium sensor Case12 (pCase12-cyto vector, Evrogen, Moscow, Russia) in a molar ratio of 2:1 following a lipofectamine transfection protocol (ThermoFisher Scientific, Waltham, MA, USA). Human α7 nAChR (α7 nAChR-pCEP4) was expressed accordingly with a co-expression of the human chaperone Ric-3 (Ric3-pCMV6-XL5, OriGene, Rockville, MD, USA) in a molar ratio 4:1. …
Showing 1-6 of 13 papers.
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