B7-2 (CD86) (NM_006889) Human Untagged Clone

SKU SC111929

CD86 (untagged)-Human CD86 molecule (CD86), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: B7-2
• Synonyms: B7-2; B7.2; B70; CD28LG2; LAB72
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006889 edited; GAATTCGGCACGAGGGGAAGGCTTGCACAGGGTGAAAGCTTTGCTTCTCTGCTGCTGTAA; CAGGGACTAGCACAGACACACGGATGAGTGGGGTCATTTCCAGATATTAGGTCACAGCAG; AAGCAGCCAAAATGGATCCCCAGTGCACTATGGGACTGAGTAACATTCTCTTTGTGATGG; CCTTCCTGCTCTCTGGTGCTGCTCCTCTGAAGATTCAAGCTTATTTCAATGAGACTGCAG; ACCTGCCATGCCAATTTGCAAACTCTCAAAACCAAAGCCTGAGTGAGCTAGTAGTATTTT; GGCAGGACCAGGAAAACTTGGTTCTGAATGAGGTATACTTAGGCAAAGAGAAATTTGACA; GTGTTCATTCCAAGTATATGGGCCGCACAAGTTTTGATTCGGACAGTTGGACCCTGAGAC; TTCACAATCTTCAGATCAAGGACAAGGGCTTGTATCAATGTATCATCCATCACAAAAAGC; CCACAGGAATGATTCGCATCCACCAGATGAATTCTGAACTGTCAGTGCTTGCTAACTTCA; GTCAACCTGAAATAGTACCAATTTCTAATATAACAGAAAATGTGTACATAAATTTGACCT; GCTCATCTATACACGGTTACCCAGAACCTAAGAAGATGAGTGTTTTGCTAAGAACCAAGA; ATTCAACTATCGAGTATGATGGTATTATGCAGAAATCTCAAGATAATGTCACAGAACTGT; ACGACGTTTCCATCAGCTTGTCTGTTTCATTCCCTGATGTTACGAGCAATATGACCATCT; TCTGTATTCTGGAAACTGACAAGACGCGGCTTTTATCTTCACCTTTCTCTATAGAGCTTG; AGGACCCTCAGCCTCCCCCAGACCACATTCCTTGGATTACAGCTGTACTTCCAACAGTTA; TTATATGTGTGATGGTTTTCTGTCTAATTCTATGGAAATGGAAGAAGAAGAAGCGGCCTC; GCAACTCTTATAAATGTGGAACCAACACAATGGAGAGGGAAGAGAGTGAACAGACCAAGA; AAAGAGAAAAAATCCATATACCTGAAAGATCTGATGAAGCCCAGCGTGTTTTTAAAAGTT; CGAAGACATCTTCATGCGACAAAAGTGATACATGTTTTTAATTAAAGAGTAAAGCCCATA; CAAGTATTCATTTTTTCTACCCTTTCCTTTGTAAGTTCCTGGGCAACCTTTTTGATTTCT; TCCAGAAGGCAAAAAGACATTACCATGAGTAATAAGGGGGCTCCAGGACTCCCTCTAAGT; GGAATAGCCTCCCTGTAACTCCAGCTCTGCTCCGTATGCCAAGAGGAGACTTTAATTCTC; TTACTGCTTCTTTTCACTTCAGAGCACACTTATGGGCCAAGCCCAGCTTAATGGCTCATG; ACCTGGAAATAAAATTTAGGACCAATACCTCCTCCAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006889 unedited; AGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGAAGGCTT; GCACAGGGTGAAAGCTTTGCTTCTCTGCTGCTGTAACAGGGACTAGCACAGACACACGGA; TGAGTGGGGTCATTTCCAGATATTAGGTCACAGCAGAAGCAGCCAAAATGGATCCCCAGT; GCACTATGGGACTGAGTAACATTCTCTTTGTGATGGCCTTCCTGCTCTCTGGTGCTGCTC; CTCTGAAGATTCAAGCTTATTTCAATGAGACTGCAGACCTGCCATGCCAATTTGCAAACT; CTCAAAACCAAAGCCTGAGTGAGCTAGTAGTATTTTGGCAGGACCAGGAAAACTTGGTTC; TGAATGAGGTATACTTAGGCAAAGAGAAATTTGACAGTGTTCATTCCAAGTATATGGGCC; GCACAAGTTTTGATTCGGACAGTTGGACCCTGAGACTTCACAATCTTCAGATCAAGGACA; AGGGCTTGTATCAATGTATCATCCATCACAAAAGAGCCCACAGGAATGATTCGCATCCAC; CAGATGAATTCTGAACTGTCAGTGCTTGCTAACTTCAGTCAACCTGAAATAGTACCAATT; TCTAATATAACAGAAAATGTGTACATAAATTTGACCTGCTCATCTATACACGGTTACCCA; GAACCTAAGAAGATGAGTGTTTTGCTAAGAACCAAGAATTCAACTATCGAGTATGATGGT; ATTATGCAGAAATCTCAAGATAATGTCACAGAACTGTACGACGTTTCCATCAGCNTTGTC; TGTTTCATTCCCTGATGTTACGAGCCATATGACCATCTTCTGTATTCTGGANACTGACAG; ACGCGGCTTTTATCTTCACCTTTCTCTATAGAGCTTGAGGACCCTCAGCCTCCCCAGACA; CATTCCTTGGATTACAGCTGACTTNCACAGTATAATGGGGGAAGAGGGTNTCTGTCTAAT; CTATGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006889 unedited; CCGTANGGTTATGGTCCTAATTTTATTTNCCAGNTCATGNAGCCATTAAGCTGGGCTTGG; NCCCATAAGTGTGCTNNCTGAAGTGAAAAAAAGCAGTAAGAGAATTAAAGTCTCCTCTTG; GCATACGGAGCAGAGCTGGAGTTACAGGGAGGCTATTCCACTTAGAGGGAGTCCTGGAGC; CCCCTTATTACTCATGGTAATGTCTTTTTGCCTTCTGGAAGAAATCAAAAAGGTTGCCCA; GGAACTTACAAAGGAAAGGGTAGAAAAAATGAATACTTGTATGGGCTTTACTCTTTAATT; AAAAACATGTATCACTTTTGTCGCATGAAGATGTCTTCGAACTTTTAAAAACACGCTGGG; CTTCATCAGATCTTTCAGGTATATGGATTTTTTCTCTTTTCTTGGTCTGTTCACTCTCTT; CCCTCTCCATTGTGTTGGTTCCACATTTATAAGAGTTGCGAGGCCGCTTCTTCTTCTTCC; ATTTCCATAGAATTAGACAGAAAACCATCACACATATAATAACTGTTGGAAGTACAGCTG; TAATCCAAGGAATGTGGTCTGGGGGAGGCTGAGGGTCCTCAAGCTCTATAGAGAAAGGTG; AAGATAAAAGCCGCGTCTTGTCAGTTTCCAGAATACAGAAGATGGTCATATTGCTCGTAA; CATCAGGGAATGAAACAGACAAGCTGATGGAAACGTCGTACAGTTCTGTGACATTATCTT; GAGATTTCTGCATAATACCATCATACTCGATAGTTGAATTCTTGGGGTCTTAGCCAACAC; TCATCTTCTTTAGTGCTGGGTAACCGTGTATAGATGAGCAAGTCAAATTTATGTACACAT; TTTTCTGTTATATTAGAAATTGGGACTATTTTCAGGTTGACTGAAGTTAGCAAGCCCTGA; CAGTTCAAAATTCATCTGTTGGATGCGAATCATTCCTGGTGGGCTTTTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006889
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006889.2, NP_008820.1
• RefSeq Size: 2794 bp
• RefSeq ORF: 2790 bp
• Locus ID: 942
• UniProt ID: P42081
• Cytogenetics: 3q13.33
• Domains: IG, IGv
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis
• Summary: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]; Transcript Variant: This variant (2) includes an alternate exon in the 5' UTR, resulting in the use of a downstream start codon compared to variant 1. Isoform 2 has a shorter N-terminus than isoform 1.