CD8A (NM_001768) Human Untagged Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | CD8A |
| Synonyms | CD8; Leu2; p32 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_001768 |
| Insert Size | 708 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001768.4, NP_001759.3 |
| RefSeq Size | 2261 bp |
| RefSeq ORF | 708 bp |
| Locus ID | 925 |
| UniProt ID | P01732 |
| Cytogenetics | 2p11.2 |
| Domains | ig, IG, IGv |
| Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
| Protein Pathways | Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway |
| Summary | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. provided by RefSeq, May 2020 Transcript Variant: This variant (1) encodes the longer, membrane associated isoform (1). Both variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
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Resources
"NM_001768" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206608 | CD8A (Myc-DDK-tagged)-Human CD8a molecule (CD8A), transcript variant 1 | 10 ug |
$300.00
|
|
| RC206608L1 | Lenti ORF clone of Human CD8a molecule (CD8A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206608L2 | Lenti ORF clone of Human CD8a molecule (CD8A), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC206608L3 | Lenti ORF clone of Human CD8a molecule (CD8A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206608L4 | Lenti ORF clone of Human CD8a molecule (CD8A), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG206608 | CD8A (tGFP-tagged) - Human CD8a molecule (CD8A), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC322577 | CD8A (untagged)-Human CD8a molecule (CD8A), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit
Choi, Koo, Yoon et al
Mol Med Rep (2020) 22 (3), 2395-2403 DOI: 10.3892/mmr.2020.11307
Choi, Koo, Yoon et al
Mol Med Rep (2020) 22 (3), 2395-2403 DOI: 10.3892/mmr.2020.11307
Cited by 5
AI Summary: OriGene's pCMV6-XL5 p32 plasmid was used as the source for p32 gene overexpression constructs, enabling the study to investigate how increased p32 levels regulate calcium signaling and endothelial nitric oxide synthase activity in endothelial cells.
Snippet: croscopy (magnification, ×10 objective lens) equipped with the Metamorph program 7.6 (Molecular Devices LLC). Preparation of p32-expressing adenovirus The p32 plasmid, pCMV6-XL5, was purchased from OriGene Technologies, Inc. and subcloned into the BglII and KpnI restriction sites of the pCMV-Tag1 plasmid. For virus generation, full-length p32 was cloned into the BamHI and XhoI sites of the pENTR
A Chimeric Antigen Receptor for TRAIL-receptor 1 induces apoptosis in various types of tumor cells
Kobayashi, Kishi, Ozawa et al
Biochemical and Biophysical Research Communications (2014)
Kobayashi, Kishi, Ozawa et al
Biochemical and Biophysical Research Communications (2014)
AI Summary: OriGene products, specifically human CD3ζ, CD8α, and CD28 cDNAs, were used as key components to construct the chimeric antigen receptor (CAR) in this study.
Snippet: and [13]. To construct the cytoplasmic domain of CAR, CD137 (4-1BB) cDNA was first isolated from human PBLs;human CD3ζ, CD8α and CD28 cDNAs were purchased from OriGene Technologies (Rockville, MD, USA). The ... Cite SaveSaving
Retroviral vectors for homologous recombination provide efficient cloning and expression in mammalian cells
Kobayashi, Kishi, Ozawa et al
Biochem. Biophys. Res. Commun. (2014)
Kobayashi, Kishi, Ozawa et al
Biochem. Biophys. Res. Commun. (2014)
Cited by 2
AI Summary: OriGene contributed to this study by providing human CD3γ, CD3δ, CD3ε, ζ, and CD8 cDNA products, which were essential for constructing expression vectors used in the cloning and functional analysis of T-cell receptors in mammalian cells.
Snippet: CD8 cDNAs were constructed by linking these cDNAs with the 2A sequence from the foot-and-mouth disease virus, as previously described (2A-hCD3-hCD8 vector) [14]. All human CD3γ, CD3δ, CD3ε, ζ and CD8 cDNAs were purchased from Origene
Relative resistance to slow inactivation of human cardiac Na+ channel hNav1.5 is reversed by lysine or glutamine substitution at V930 in D2-S6
Chancey, Shockett, O'Reilly
Am J Physiol Cell Physiol (2007) 293 (6), C1895-905 DOI: 10.1152/ajpcell.00377.2007
Chancey, Shockett, O'Reilly
Am J Physiol Cell Physiol (2007) 293 (6), C1895-905 DOI: 10.1152/ajpcell.00377.2007
Cited by 17
AI Summary: OriGene products contributed to this study by providing the human SCN5A (hNav1.5) cDNA (OriGene #SC118560), which was used for generating channel mutants and expressing them in HEK cells for electrophysiological analysis.
Showing 1-4 of 4 papers.
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