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SEM1 (NM_006304) Human Untagged Clone

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SKU
SC111062
SHFM1 (untagged)-Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1)
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SEM1
Synonyms C7orf76; DSS1; ECD; PSMD15; SHFD1; Shfdg1; SHFM1; SHSF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111062 sequence for NM_006304 edited (data generated by NextGen Sequencing)
ATGTCAGAGAAAAAGCAGCCGGTAGACTTAGGTCTGTTAGAGGAAGACGACGAGTTTGAA
GAGTTCCCTGCCGAAGACTGGGCTGGCTTAGATGAAGATGAAGATGCACATGTCTGGGAG
GATAATTGGGATGATGACAATGTAGAGGATGACTTCTCTAATCAGTTACGAGCTGAACTA
GAGAAACATGGTTATAAGATGGAGACTTCATAG

Clone variation with respect to NM_006304.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006304 unedited
TGGGGGTTCGNATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGG
AGGGTTCCAACTTTTCTGCTTATCTGGGAGGTGTTGGGCGCGGACAGTCGAGATGTCAGA
GAAAAAGCAGCCGGTAGACTTAGGTCTGTTAGAGGAAGACGACGAGTTTGAAGAGTTCCC
TGCCGAAGACTGGGCTGGCTTAGATGAAGATGAAGATGCACATGTCTGGGAGGATAATTG
GGATGATGACAATGTAGAGGATGACTTCTCTAATCAGTTACGAGCTGAACTAGAGAAACA
TGGTTATAAGATGGAGACTTCATAGCATCCAGAAGAAGTGTTGAAGTAACCTAAACTTGA
CCTGCTTAATACATTCTAGGGCAGAGAACCCAGGATGGGACACTAAAAAAATGTGTTTAT
TTCATTATCTGCTTGGATTTATTTGTGTTTTTGTAACACAAAAAATAAATGTTTTGATAT
AATCTTGGTTTGGTTCAGACCACACACACCACCCCCCCCCCCCCCCCCACCCACCCACCC
AAAAAAACTCGACTCTAGATTGCGGCCGCGGCCATAGCTGTTTCCTGAACAGATCCCGGG
TGGCATCCCTGTGACCCCTCCCCAGTGCCTTTCCTGGCCCTGGAAGTTGCCACTCCAGTG
CCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGGGTCCTTC
TATATATTATGGGGTGGGGGGGGGGCGTTGGAGCCAGGGGCCATTTGGGAAAACACCCGC
ACGGCCCTCGGGGCCTTATTGGACCCAACCGGAACCGCCGGCCCATCCTGGCTCCTGCAT
TCCCCCCCCCCGGGTCCAAGCCATTTCCCTGCCCCACCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006304 unedited
ATGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTCGGAACCAAACCAAAATTATTTCAAAACATTTATTTTTTGGGT
TACAAAAACACAAATAAATCCAAGCAGATAATGAAATAAACACATTTTTTTAGGGGCCCA
TCCTGGGTTCTCTGCCCTAAAATGTATTAAGCAGGTCAAGTTTAGGTTACTTCAACACTT
TTTTTGGATGCTATGAAGTCTCCATTTTATAACCATGTTTCTTTAGTTCAGCTCGAAACT
GATTAAAAAAGTCATCCTTTACATTGGCATCATCCCAATTATCCTCCCAAACATGGGCAT
TTTCATTTTCATCTAAGCCAGCCCAGTCTTCGGGAGGGAACTTTTAAAACTCGTCGTTTT
CCTTTAACAAACCTAAGTCTACCGGCTGGTTTTTTTTTGACATTTGGACTGGCCGCGCCC
AACACCTCCCAAATAAGCAAAAAAGTTGGAACCCTCCCTCGTGCCGAATTCGCGGCCGCC
CTATAGGGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAAA
CCTCCCACCGTACACGCCTACCGCCCATTTGGGTCAACGGGGCGGGGTTATTACCACATT
TTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGG
AAACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGTTGTACTGCCAAAAC
CGCATCACCATGGTAATAGCGATGACTAAACNTANATGTACTGCCAAGTAGAAAGTCCCG
TAGGTCATGTACTGGCATAAATGCAGCGGGCCATTTACCGCANTGACGTCAATAGGGGCG
ACTNGCATAAGAACCTGGATGC
Restriction Sites NotI-NotI |
ACCN NM_006304
Insert Size 520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006304.1, NP_006295.1
RefSeq Size 509 bp
RefSeq ORF 213 bp
Locus ID 7979
UniProt ID P60896
Cytogenetics 7q21.3
Domains DSS1_SEM1
Protein Pathways Homologous recombination, Proteasome
Summary The product of this gene has been localized within the split hand/split foot malformation locus SHFM1 at chromosome 7. It has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. provided by RefSeq, Jul 2008
Transcript Variant: This variant (5) differs in the 3' exon structure and the entire coding region, compared to variant 1. It encodes isoform 3, which has a completely different sequence, compared to isoform 1.
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Documents
Resources
"NM_006304" in other vectors (6)
SKU Description Size Price
RC207564 SHFM1 (Myc-DDK-tagged)-Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1) 10 ug
$150.00
RC207564L1 Lenti ORF clone of Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1), Myc-DDK-tagged 10 ug
$450.00
RC207564L2 Lenti ORF clone of Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1), mGFP tagged 10 ug
$450.00
RC207564L3 Lenti ORF clone of Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1), Myc-DDK-tagged 10 ug
$450.00
RC207564L4 Lenti ORF clone of Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1), mGFP tagged 10 ug
$450.00
RG207564 SHFM1 (tGFP-tagged) - Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1) 10 ug
$489.00

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