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Vimentin (VIM) (NM_003380) Human Untagged Clone

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SKU
SC111054
VIM (untagged)-Human vimentin (VIM)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Vimentin
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111054 sequence for NM_003380 edited (data generated by NextGen Sequencing)
ATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGCGGCCCGGGCACC
GCGAGCCGGCCGAGCTCCAGCCGGAGCTACGTGACTACGTCCACCCGCACCTACAGCCTG
GGCAGCGCGCTGCGCCCCAGCACCAGCCGCAGCCTCTACGCCTCGTCCCCGGGCGGCGTG
TATGCCACGCGCTCCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGCGGCTCCTG
CAGGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACACCGAGTTCAAGAACACCCGC
ACCAACGAGAAGGTGGAGCTGCAGGAGCTGAATGACCGCTTCGCCAACTACATCGACAAG
GTGCGCTTCCTGGAGCAGCAGAATAAGATCCTGCTGGCCGAGCTCGAGCAGCTCAAGGGC
CAAGGCAAGTCGCGCCTGGGGGACCTCTACGAGGAGGAGATGCGGGAGCTGCGCCGGCAG
GTGGACCAGCTAACCAACGACAAAGCCCGCGTCGAGGTGGAGCGCGACAACCTGGCCGAG
GACATCATGCGCCTCCGGGAGAAATTGCAGGAGGAGATGCTTCAGAGAGAGGAAGCCGAA
AACACCCTGCAATCTTTCAGACAGGATGTTGACAATGCGTCTCTGGCACGTCTTGACCTT
GAACGCAAAGTGGAATCTTTGCAAGAAGAGATTGCCTTTTTGAAGAAACTCCACGAAGAG
GAAATCCAGGAGCTGCAGGCTCAGATTCAGGAACAGCATGTCCAAATCGATGTGGATGTT
TCCAAGCCTGACCTCACGGCTGCCCTGCGTGACGTACGTCAGCAATATGAAAGTGTGGCT
GCCAAGAACCTGCAGGAGGCAGAAGAATGGTACAAATCCAAGTTTGCTGACCTCTCTGAG
GCTGCCAACCGGAACAATGACGCCCTGCGCCAGGCAAAGCAGGAGTCCACTGAGTACCGG
AGACAGGTGCAGTCCCTCACCTGTGAAGTGGATGCCCTTAAAGGAACCAATGAGTCCCTG
GAACGCCAGATGCGTGAAATGGAAGAGAACTTTGCCGTTGAAGCTGCTAACTACCAAGAC
ACTATTGGCCGCCTGCAGGATGAGATTCAGAATATGAAGGAGGAAATGGCTCGTCACCTT
CGTGAATACCAAGACCTGCTCAATGTTAAGATGGCCCTTGACATTGAGATTGCCACCTAC
AGGAAGCTGCTGGAAGGCGAGGAGAGCAGGATTTCTCTGCCTCTTCCAAACTTTTCCTCC
CTGAACCTGAGGGAAACTAATCTGGATTCACTCCCTCTGGTTGATACCCACTCAAAAAGG
ACACTTCTGATTAAGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAG
CATCACGATGACCTTGAATAA

Clone variation with respect to NM_003380.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003380 unedited
GGTTTAGGATTTGTAACCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGCTC
CGAGGTCCCCGCGCCAGAGACGCAGCCGCGCTCCCACCACCCACACCCACCGCGCCCTCG
TTCGCCTCTTCTCCGGGAGCCAGTCCGCGCCACCGCCGCCGCCCAGGCCATCGCCACCCT
CCGCAGCCATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGCGGCC
CGGGCACCGCGAGCCGGCCGAGCTCCAGCCGGAGCTACGTGACTACGTCCACCCGCACCT
ACAGCCTGGGCAGCGCGCTGCGCCCCAGCACCAGCCGCAGCCTCTACGCCTCGTCCCCGG
GCGGCGTGTATGCCACGCGCTCCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGC
GGCTCCTGCAGGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACACCGAGTTCAAGA
ACACCCGCACCAACGAGAAGGTGGAGCTGCAGGAGCTGAATGACCGCTTCGCCAACTACA
TCGACAAGGTGCGCTTCCTGGAGCAGCAGAATAAGATCCTGCTGGCCGAGCTCGAGCAGC
TCAAGGGCCAAGGCAAGTCGCGCCTGGGGGACCTCTACGAGGAGGAGATGCGGGAGCTGC
GCCGGCAGGTGGACCAGCTAACCAACGANCAAGCCCGCGTCGAGGTGGAGCGCGANCACC
TGGNCCGAGACATCATGCGCCTNCGGGAGAAATTGCAGGANGAGATGCTTCAGAGAGAGG
AAGCCGAAACACCCTGCAATCTTTCAGACAGGGATGTGACNATGCGTCTCTGGCACGTCT
TGACCTTGAACGCAAAGTGGAATCTTTGCAAGAAGAGATTGCCCCTTTTGAAGAAACTCC
NCCGAAAGGAAATCCAGGAGCTG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003380 unedited
ATGGACCGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGTTTTTCCAA
AGATTATTGAAGCAGAACCAAGTTGGTTGGATACTTGCTGGAAAAAAAAACAGTTTTAAT
GGTATTCAAAATACCTTTTAAAAAGTATTCTAGCACAAGATTTTTCTGTAAACTAGATTA
TGTTGTAAACTTTTTTCTAAATCTTGTAGGAGTGTCGGTTGTTAAGAACTAGAGCTTATT
CCTATTCCAAATCTATCTTGCGCTCCTGAAAAACTGCAGAAAGGCACTTGAAAGCTGTTT
CTTTAAGATATGGATTTCTTTTTTATTCTTGCTGGTAATATATTGCTGCACTGAGTGTGT
GCAATTTTTATTCAAGGTCATCGTGATGCTGAGAAGTTTCGTTGATAACCTGTCCATCTC
TAGTTTCAACCGTCTTAATCAGAAGTGTCCTTTTTGAGTGGGTATCAACCAGAGGGAGTG
AATCCAGATTAGTTTCCCTCAGGTTCAGGGAGGAAAAGTTTGGAAGAGGCAGAGAAATCC
TGCTCTCCTCGCCTTCCAGCAGCTTCCTGTAGGTGGCAATCTCAATGTCAAGGGCCATCT
TAACATTGAGCAGGTCTTGGTATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCT
GAATCTCATCCTGCAGGCGGCCAATAGTGTCTTGGTAGTTAGCAGCTTCAACGGCAAAGT
TCTCTTCCATTTCACGCATCTGGCGTTCCAGGAACTCATTGGTTCCTTTAGGGCCTCCAC
TCACAGGTGAAGGACTGCACCTGTCTCCGGACTTAGTGGACTCCTGCCTTGCCTGCCGCA
AGCGCGTATTGTTCCGGTTGGCAACCTAAAGAGTCAGCAACTCGGATTTGCCT
Restriction Sites NotI-NotI |
ACCN NM_003380
Insert Size 1401 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003380.2, NP_003371.2
RefSeq Size 1847 bp
RefSeq ORF 1401 bp
Locus ID 7431
UniProt ID P08670, V9HWE1
Cytogenetics 10p13
Domains filament, filament_head
Protein Families ES Cell Differentiation/IPS
Summary This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. provided by RefSeq, Aug 2017
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Documents
Resources
"NM_003380" in other vectors (6)
SKU Description Size Price
RC201546 VIM (Myc-DDK-tagged)-Human vimentin (VIM) 10 ug
$457.00
RC201546L1 Lenti ORF clone of Human vimentin (VIM), Myc-DDK-tagged 10 ug
$757.00
RC201546L2 Lenti ORF clone of Human vimentin (VIM), mGFP tagged 10 ug
$757.00
RC201546L3 Lenti ORF clone of Human vimentin (VIM), Myc-DDK-tagged 10 ug
$757.00
RC201546L4 Lenti ORF clone of Human vimentin (VIM), mGFP tagged 10 ug
$757.00
RG201546 VIM (tGFP-tagged) - Human vimentin (VIM) 10 ug
$489.00 $657.00

file-alt Citations

Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration
Nowicki, Hayes, Chiocca et al
Cancers (Basel) (2019) 11 (4) DOI: 10.3390/cancers11040466
AI Summary: In this study, the human vimentin (SC111054) product from OriGene was used to investigate the role of vimentin in glioblastoma cell migration, helping to demonstrate that vimentin downregulation impairs cell migration and is affected by GSK-3 inhibition.
Snippet: The pcDNA4TO plasmid was purchased from Thermo-Fisher/Life Technologies (cat# V1020), human vimentin was obtained from OriGene (SC111054), GSK-3α/β coding sequences were provided by Dr. Chris Phiel, Nationwide Children's Hospital, Columbus, OH;The pcDNA4TO plasmid was purchased from Thermo-Fisher/Life Technologies (cat# V1020), human vimentin was obtained from OriGene (SC111054), GSK-3α/β coding sequences were provided by Dr. Chris Phiel, Nationwide Children's Hospital, Columbus, OH Cached
Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
Chu, Haynes, Corbel et al
Nat. Methods (2014)
Cited by 255
AI Summary: In this study, OriGene products—specifically human pyruvate dehydrogenase (NM_000284), human peroxisomal membrane protein (NM_018663.1), and human vimentin (NM_003380.3)—were used as genetic templates to generate fluorescent protein fusion constructs for validating and visualizing cellular differentiation in vivo.
Snippet: human keratin 18 (NM_199187.1, Open Biosystems), EcoRI and NotI;yeast Lifeact (IDT DNA), BamHI and NotI;human pyruvate dehydrogenase, (NM_000284, OriGene), AgeI and NotI;human peroxisomal membrane protein (NM_018663.1, OriGene), NotI and AgeI;human vimentin (NM_003380.3, OriGene), NheI and BamHI;chicken paxillin (NM_204984.1, C. Waterman, NIH), NheI and BamHI;mouse mannosidase II (NM_008549.2, J. …
Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes
York, Ghitani, Vaziri et al
Nat. Methods (2011) 8 (4), 327-33 DOI: 10.1038/nmeth.1571
Cited by 172
AI Summary: OriGene contributed to this study by providing the cDNA for the mitochondrial targeting signal (MTS) of human cytochrome C oxidase subunit VIII (product: NM_004074.2), which was used to generate fusion constructs for super-resolution imaging of mitochondrial structures.
Snippet: left to right on a picosecond timescale, illuminating a line, and a galvanometric mirror scans the illuminated line up-down on a ... cDNA for the mitochondrial targeting signal (MTS) of human cytochrome C oxidase subunit VIII (cDNA source: Origene;NM_004074.2) was amplified
FigureFigureFigure
Showing 1-4 of 4 papers.
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