Prohibitin (PHB) (NM_002634) Human Untagged Clone

SKU SC110973

PHB (untagged)-Human prohibitin (PHB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Prohibitin
• Synonyms: HEL-215; HEL-S-54e; PHB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002634 edited; GAATTCGGCACGAGGGGAATTCATGTGGAGGTCAGAGTGGAAGCAGGTGTGAGAGGGTCC; AGCAGAAGGAAACATGGCTGCCAAAGTGTTTGAGTCCATTGGCAAGTTTGGCCTGGCCTT; AGCTGTTGCAGGAGGCGTGGTGAACTCTGCCTTATATAATGTGGATGCTGGGCACAGAGC; TGTCATCTTTGACCGATTCCGTGGAGTGCAGGACATTGTGGTAGGGGAAGGGACTCATTT; TCTCATCCCGTGGGTACAGAAACCAATTATCTTTGACTGCCGTTCTCGACCACGTAATGT; GCCAGTCATCACTGGTAGCAAAGATTTACAGAATGTCAACATCACACTGCGCATCCTCTT; CCGGCCTGTCGCCAGCCAGCTTCCTCGCATCTTCACCAGCATCGGAGAGGACTATGATGA; GCGTGTGCTGCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTTGATGC; TGGAGAACTAATCACCCAGAGAGAGCTGGTCTCCAGGCAGGTGAGCGACGACCTTACAGA; GCGAGCCGCCACCTTTGGGCTCATCCTGGATGACGTGTCCTTGACACATCTGACCTTCGG; GAAGGAGTTCACAGAAGCGGTGGAAGCCAAACAGGTGGCTCAGCAGGAAGCAGAGAGGGC; CAGATTTGTGGTGGAAAAGGCTGAGCAACAGAAAAAGGCGGCCATCATCTCTGCTGAGGG; CGACTCCAAGGCAGCTGAGCTGATTGCCAACTCACTGGCCACTGCAGGGGATGGCCTGAT; CGAGCTGCGCAAGCTGGAAGCTGCAGAGGACATCGCGTACCAGCTCTCACGCTCTCGGAA; CATCACCTACCTGCCAGCGGGGCAGTCCGTGCTCCTCCAGCTGCCCCAGTGAGGGCCCAC; CCTGCCTGCACCTCCGCGGGCTGACTGGGCCACAGCCCCGATGATTCTTAACACAGCCTT; CCTTCTGCTCCCACCCCAGAAATCACTGTGAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002634 unedited; GCGTCAGGAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTC; GTGCCGAATTCGGCACGAGGGGAATTCATGTGGAGGTCAGAGTGGAAGCAGGTGTGAGAG; GGTCCAGCAGAAGGAAACATGGCTGCCAAAGTGTTTGAGTCCATTGGCAAGTTTGGCCTG; GCCTTAGCTGTTGCAAGAGGCGTGGTGAACTCTGCCTTATATAATGTGGATGCTGGGCAC; AGAGCTGTCATCTTTGACCGATTCCGTGGAGTGCAGGACATTGTGGTAGGGGAAGGGACT; CATTTTCTCATCCCGTGGGTACAGAAACCAATTATCTTTGACTGCCGTTCTCGACCACGT; AATGTGCCAGTCATCACTGGTAGCAAAGATTTACAGAATGTCAACATCACACTGCGCATC; CTCTTCCGGCCTGTCGCCAGCCAGCTTCCTCGCATCTTCACCAGCATCGGAGAGGACTAT; GATGAGCGTGTGCTGCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTT; GATGCTGGAGAACTAATCACCCAGAGAGAGCTGGTCTCCAGGCAGGTGAGCGACGACCTT; ACAGAGCGAGCCGCCACCTTTGGGCTCATCCTGGATGACGTGTCCTTGACACATCTGACC; TTCGGGAAGGAGTTCACAGAAGCGGTGGAAGCCAAACAGGTGGCTCATCATGAAGCAGAG; AGGGCCAGATTTGTGGTGGAAAATGCTGAGNCACAGAAAAGGCGGCCATCATCTCTGCTG; NAGGGCGACTCCAGGCAGCTGAGCTGATTGCAACTCACTGGCACTGCAGGGGATGGCCTG; ATCGAGCTGCGCAAGCTGGAAGCTGCAGAGGACATCGCGTACCAGCTCTAACGCTCTCGG; GACATCACCTACCTGGCAGCGGGGCATGTCGTGCNTCTNCAGCTGCCCCAGTGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002634 unedited; CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTGATTTTACCTTTATTT; CCTTCACTTTAAGCCAATCATGAAATTTCACAGTGATTTCTGGGGTGGGAGCAGAAGGAA; GGCTGTGTTAAGAATCATCGGGGCTGTGGCCCAGTCAGCCCGCGGAGGTGCAGGCAGGGT; GGGCCCTCACTGGGGCAGCTGGAGGAGCACGGACTGCCCCGCTGGCAGGTAGGTGATGTT; CCGAGAGCGTGAGAGCTGGTACGCGATGTCCTCTGCAGCTTCCAGCTTGCGCAGCTCGAT; CAGGCCATCCCCTGCAGTGGCCAGTGAGTTGGCAATCAGCTCAGCTGCCTTGGAGTCGCC; CTCAGCAGAGATGATGGCCGCCTTTTTCTGTTGCTCAGCCTTTTCCACCACAAATCTGGC; CCTCTCTGCTTCCTGCTGAGCCACCTGTTTGGGCTCCACCGCTTCTTGGGACTCCCTTTC; CGAAGGCAAAAAGGTTAAAGGCCCCCTTTTCCCCAAAAGACCCACAGGGGGGCGCTCCCC; CTTTAAGGGGCCCCCTCCCCCCCCGGAAACCCACCCTCTTGGGGAGAAATTTCCCCACAA; AAGAGACCCCCCCCGCCTTTGGGGCCCCCCGGGGGGGGGGGGGCCCCCCCCCCATATACC; CCCCCCCCACCGGAAAAAAAACAAAAAAAATTTTGGCCCACCCCCCCAAAAAAAACCCCC; CCCCCGGGGGCACACCCTTTAAAATTTTTCCCCCCCGGGAGGGGGGCCCCCCCGGGGGGA; GCCCCCCCCAAAAAAAAATTTTTTTCTTCCCCCCCCCCCCAAGAAAAAAAAAATTTCCTC; TCCCCCACAAGAGGGGCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002634
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002634.2, NP_002625.1
• RefSeq Size: 1826 bp
• RefSeq ORF: 819 bp
• Locus ID: 5245
• UniProt ID: P35232
• Cytogenetics: 17q21.33
• Domains: Band_7
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Summary: This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1).