Prohibitin (PHB) (NM_002634) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Prohibitin |
| Synonyms | HEL-215; HEL-S-54e; PHB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002634 |
| ORF Size | 816 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002634.4 |
| RefSeq Size | 1826 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 5245 |
| UniProt ID | P35232 |
| Cytogenetics | 17q21.33 |
| Domains | Band_7 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| MW | 29.6 kDa |
| Summary | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002634" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201229L1 | Lenti ORF clone of Human prohibitin (PHB), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201229L2 | Lenti ORF clone of Human prohibitin (PHB), mGFP tagged | 10 ug |
$600.00
|
|
| RC201229L3 | Lenti ORF clone of Human prohibitin (PHB), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201229L4 | Lenti ORF clone of Human prohibitin (PHB), mGFP tagged | 10 ug |
$600.00
|
|
| RG201229 | PHB (tGFP-tagged) - Human prohibitin (PHB) | 10 ug |
$489.00
$500.00
|
|
| SC110973 | PHB (untagged)-Human prohibitin (PHB) | 10 ug |
$300.00
|
|
Citations
GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease
Xu, Chang, Zhou et al
J Exp Med (2021) 218 (8) DOI: 10.1084/jem.20202446
Xu, Chang, Zhou et al
J Exp Med (2021) 218 (8) DOI: 10.1084/jem.20202446
Cited by 33
AI Summary: OriGene products, including Myc-Flag–tagged Arcn1 (RC210778), PHB (RC201229), Erlin2 (RC221700), mouse full-length GSAP with HA tag (EX-Mm30424-M07), human GSAP plasmids (EX-Z2830-M07), and Fe65 with Flag tag, were used for protein expression and functional studies to investigate GSAP's role in lipid homeostasis and mitochondrial function in Alzheimer's disease.
Snippet: (Life Technologies) were used for all transient transfections following the manufacturer’s instructions. Myc-Flag–tagged Arcn1 (RC210778), PHB (RC201229), and Erlin2 (RC221700) were obtained from OriGene. Mouse full-length GSAP with HA tag (EX-Mm30424-M07), human GSAP plasmids (EX-Z2830-M07), human GSAP-16k with HA tag (aa 733–854 subcloned from full-length HA-GSAP), Fe65 with Flag tag (EX-M
The mitochondrial chaperone Prohibitin 1 negatively regulates interleukin-8 in human liver cancers
Yang, Murray, Barbier-Torres et al
J. Biol. Chem. (2018) 294 (6), 1984-1996 DOI: 10.1074/jbc.RA118.004863
Yang, Murray, Barbier-Torres et al
J. Biol. Chem. (2018) 294 (6), 1984-1996 DOI: 10.1074/jbc.RA118.004863
AI Summary: OriGene’s PHB1 overexpression plasmid and empty vector were used to manipulate PHB1 levels in liver cancer cells, enabling the authors to study how PHB1 regulates IL-8 expression and tumorigenic processes.
Snippet: Huh7 and Hep3B cells (2x105) were forward transfected with 2 µg PHB1 overexpression or empty vector (Origene, Rockville, MD) for 48 hours using JetPRIME reagent (Polyplus-transfection, Radnor, PA) according to manufacturer's instructions
Prohibitin plays a critical role in Enterovirus 71 neuropathogenesis
Too, Bonne, Tan et al
PLoS Pathog. (2018) 14 (1), e1006778 DOI: 10.1371/journal.ppat.1006778
Too, Bonne, Tan et al
PLoS Pathog. (2018) 14 (1), e1006778 DOI: 10.1371/journal.ppat.1006778
AI Summary: OriGene’s pCMV6-PHB plasmid was used in this study to overexpress prohibitin in neuronal cells, enabling the authors to investigate its role in Enterovirus 71 infection and neuropathogenesis.
Snippet: A constructs. The culture supernatant was harvested at different time points for viral titer determination. Luciferase EV71 replicon and pCMV6-PHB plasmid transfection LucEV71 replicon or pCMV6-PHB (Origene)-harbouring E. coli was grown in LB broth supplemented with kanamycin (50 μg/mL). Both plasmids were extracted using QIAprep Spin Miniprep Kit (Qiagen). LucEV71 was linearized with Mlu1 restr;...
Prohibitin 1 suppresses liver cancer tumorigenesis in mice and human hepatocellular and cholangiocarcinoma cells
Fan, Yang, Liu et al
Hepatology (2017) 65 (4), 1249-1266 DOI: 10.1002/hep.28964
Fan, Yang, Liu et al
Hepatology (2017) 65 (4), 1249-1266 DOI: 10.1002/hep.28964
AI Summary: In this study, OriGene products including the PHB1 cDNA clone (OriGene, SC119963) and PHB1 shRNA (OriGene, TL313207) were used to manipulate PHB1 expression in cell lines, enabling the authors to investigate the role of PHB1 in regulating liver cancer cell growth and gene expression.
Snippet: Prohibitin 1 (PHB1) is best known as a mitochondrial chaperone, and its role in cancer is conflicting. Mice lacking methionine adenosyltransferase α1 (MATα1) have lower PHB1 expression, and we reported that c-MYC interacts directly with both proteins. Furthermore, c-MYC and MATα1 exert opposing effects on liver cancer growth, prompting us to examine the interplay between PHB1, MATα1, and c-MYC and...
Prohibitin Overexpression in Adipocytes Induces Mitochondrial Biogenesis, Leads to Obesity Development, and Affects Glucose Homeostasis in a Sex-Specific Manner
Ande, Nguyen, Padilla-Meier et al
Diabetes (2014)
Ande, Nguyen, Padilla-Meier et al
Diabetes (2014)
Cited by 70
AI Summary: OriGene’s full-length PHB clone was used to generate transgenic mice overexpressing prohibitin in adipocytes, enabling the study of PHB’s role in mitochondrial biogenesis, obesity development, and glucose homeostasis.
Snippet: RESEARCH DESIGN AND METHODS Cloning of PHB The aP2 promoter-containing vector [pBS-aP2 promoter (5.4 kb) polyA] was obtained from Addgene (Cambridge, MA). A full-length PHB clone was obtained from OriGene Technologies (Rockville, MD). ... Cite SaveSaving
Showing 1-5 of 5 papers.
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