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EGLN2 (NM_080732) Human Untagged Clone

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SKU
SC110740
EGLN2 (untagged)-Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EGLN2
Synonyms EIT-6; EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_080732, the custom clone sequence may differ by one or more nucleotides


ATGGACAGCCCGTGCCAGCCGCAGCCCCTAAGTCAGGCTCTCCCTCAGTTACCAGGGTCTTCGTCAGAGC
CCTTGGAGCCTGAGCCTGGCCGGGCCAGGATGGGAGTGGAGAGTTACCTGCCCTGTCCCCTGCTCCCCTC
CTACCACTGTCCAGGAGTGCCTAGTGAGGCCTCGGCAGGGAGTGGGACCCCCAGAGCCACAGCCACCTCT
ACCACTGCCAGCCCTCTTCGGGACGGTTTTGGCGGGCAGGATGGTGGTGAGCTGCGGCCGCTGCAGAGTG
AAGGCGCTGCAGCGCTGGTCACCAAGGGGTGCCAGCGATTGGCAGCCCAGGGCGCACGGCCTGAGGCCCC
CAAACGGAAATGGGCCGAGGATGGTGGGGATGCCCCTTCACCCAGCAAACGGCCCTGGGCCAGGCAAGAG
AACCAGGAGGCAGAGCGGGAGGGTGGCATGAGCTGCAGCTGCAGCAGTGGCAGTGGTGAGGCCAGTGCTG
GGCTGATGGAGGAGGCGCTGCCCTCTGCGCCCGAGCGCCTGGCCCTGGACTATATCGTGCCCTGCATGCG
GTACTACGGCATCTGCGTCAAGGACAGCTTCCTGGGGGCAGCACTGGGCGGTCGCGTGCTGGCCGAGGTG
GAGGCCCTCAAACGGGGTGGGCGCCTGCGAGACGGGCAGCTAGTGAGCCAGAGGGCGATCCCGCCGCGCA
GCATCCGTGGGGACCAGATTGCCTGGGTGGAAGGCCATGAACCAGGCTGTCGAAGCATTGGTGCCCTCAT
GGCCCATGTGGACGCCGTCATCCGCCACTGCGCAGGGCGGCTGGGCAGCTATGTCATCAACGGGCGCACC
AAGGCCATGGTGGCGTGTTACCCAGGCAACGGGCTCGGGTACGTAAGGCACGTTGACAATCCCCACGGCG
ATGGGCGCTGCATCACCTGTATCTATTACCTGAATCAGAACTGGGACGTTAAGGTGCATGGCGGCCTGCT
GCAGATCTTCCCTGAGGGCCGGCCCGTGGTAGCCAACATCGAGCCACTCTTTGACCGGTTGCTCATTTTC
TGGTCTGACCGGCGGAACCCCCACGAGGTGAAGCCAGCCTATGCCACCAGGTACGCCATCACTGTCTGGT
ATTTTGATGCCAAGGAGCGGGCAGCAGCCAAAGACAAGTATCAGCTAGCATCAGGACAGAAAGGTGTCCA
AGTACCTGTATCACAGCCGCCTACGCCCACCTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_080732 unedited
TAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGCCGGAG
GAAAAACTCGCCACCCTGAAGGTCCCTTCCCAAGCCCTTAGGGACCGCAGAGGACTTGGG
GACCAGCAAGCAACCCCCAGGGCACGAGAAGAGCTCTTGCTGTCTGCCCTGCCTCACCCT
GCCCCACGCCAGGCCCGGTGGCCCCCAGCTGCATCAAGTGGAGGCGGAGGAGGAGGCGGA
GGAGGGTGGCACCATGGGCCCGGGCGGTGCCCTCCATGCCCGGGGGATGAAGACACTGCT
GCCATGGACAGCCCGTGCCAGCCGCAGCCCCTAAGTCAGGCTCTCCCTCAGTTACCAGGG
TCTTCGTCAGAGCCCTTGGAGCCTGAGCCTGGCCGGGCCAGGATGGGAGTGGAGAGTTAC
CTGCCCTGTCCCCTGCTCCCCTCCTACCACTGTCCAGGAGTGCCTAGTGAGGCCTCGGCA
GGGAGTGGGACCCCCAGAGCCACAGCCACCTCTACCACTGCCAGCCCTCTTCGGGACGGT
TTTGGCGGGCAGGATGGTGGTGAGCTGCGGCCGCTGCAGAGTGAAGGCGCTGCAGCGCTG
GTCACCAAGGGGTGCCAGCGATTGGCAGCCCAGGGCGCACGGGCCTGAGCCCCCAAACGG
AAATGGGCCGANGATGGTGGGGATGCCCCTTCACCAGCAAACGGCCCTGGGCCAGGCAAG
AGAACCANGANGCANAGCGGGAGGGTGGCATGAGCTGCAGCTGCAGCAGTGGCAGTGGTG
AAGCCAGNNTGCTGGCTGATGGAGAAGCGCTGNCCTCTGCGCCCGAGCGCCTGCCCTGGA
CTATATCGTGCCCTGCATGCNGTACTACNGCATCTGCGTCAAGGACAGCTTNCTGGGGGC
ANCACTGGNCNGTTCNCGTGCTTGGCCGAGGTGNAGGCCCTCAAAACGGGTGTGCCGCCT
GCNAAACCGGCANCTAGTGAGGCANATGGCGATCCNGNCGNGCAGCATCCGTGGGGACC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_080732 unedited
TGAACCGCCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGAGTGTGCAGC
TCTGAGGTAAACTTTTATTTCTTGGCTGACAGGACTTCCACACCCATGCTCCTCCTGGCA
CCTTTTACTCTGCCCAAGCAGTGGCAGCCCAGTCCAAAAGGTGCCTGCCATGGCCAAAGG
GGTTGGGGAGTCACTTCACATTCCAGGCTGGAGGAGTGAGAGGACTTGGGGGTACTCCTC
CCACCCCATTGCCAGCAAGAGGCCATGACTCACCTCAACCCCAAGTCCTGCATCCTGGTG
GGAAGTGACAATGCCTCCTCCCAGCTCTCCATACCTAATGGGGTCATGACCAGACAGGGT
AGGACCCAGGCCCCCAGCTTCAGTTGGTAACGGTGGCCTCCACGGCTGCCCCCTGTCCAG
GTGACAACCCCAGCCCCCATGATGGGGCAGCAAAGGTCTCTTCTCCTCCTTGGTCCTCAG
CAACAGACAGAGCCCTCCACACCAGGCAGGACAGAGGCAAAGTCAGAAGCAGCAGCGGTG
GCAGGGAAGGAGCAGCCAGCACAGGCCCAGGGCTCTCCTGAAGTCATTTAAGCTGTCTGC
CATGCGGCTCTGGGACTGGCCACTAGGTGGGCGTAGGCGGCTGTGATACAGGTACTTGGA
CACCTTTCTGTCCTGATGCTAGCTGATACTTGTCTTTGGCTGCTGCCCGCTCCTTGGCAT
CAAAATACCAGACAGTGATGGCGTACCTGGTGGCATANGCTGGCTTCACCTCGTGGGGGT
TCCGCCGGTCAGACCCAAAATGAGCAACCCGTCAAAGATGGCTCGATGTGGCTACACGGG
CCGGCCCTCAGGGAAGATCTGAGCAGGCCGCCTGCACCTTACGTCCCATTTCTGATCAGG
TATAAATACGGTGATGCAACCCCCATTCCCTGGGGATTGTAACGTGCCTTAGTACCCAAG
CCGTTGCCTGGTTAACACCACCAGGCCTGGTGCCCCC
Restriction Sites NotI-NotI |
ACCN NM_080732
Insert Size 1750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080732.1, NP_542770.1
RefSeq Size 2174 bp
RefSeq ORF 378 bp
Locus ID 112398
UniProt ID Q96KS0
Cytogenetics 19q13.2
Domains 2OG-FeII_Oxy, P4Hc
Protein Families Druggable Genome
Protein Pathways Pathways in cancer, Renal cell carcinoma
Summary The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. provided by RefSeq, Feb 2011
Transcript Variant: This variant (3) represents the longer transcript. Both variants 1 and 3 utilize alternative start codons that result in expression of two isoforms known as PHD1p43 and PHD1p40.
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Documents
Resources
"NM_080732" in other vectors (5)
SKU Description Size Price
RC219191 EGLN2 (Myc-DDK-tagged)-Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3 10 ug
$457.00
RC219191L3 Lenti ORF clone of Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC219191L4 Lenti ORF clone of Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3, mGFP tagged 10 ug
$757.00
RG219191 EGLN2 (tGFP-tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3 10 ug
$489.00 $657.00
SC317651 EGLN2 (untagged)-Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3 10 ug
$503.00

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