KRAS (NM_004985) Human Untagged Clone
SKU
SC109374
KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | KRAS |
| Synonyms | C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_004985 |
| Insert Size | 2067 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004985.3, NP_004976.2 |
| RefSeq Size | 5312 bp |
| RefSeq ORF | 567 bp |
| Locus ID | 3845 |
| UniProt ID | P01116 |
| Cytogenetics | 12p12.1 |
| Domains | RAB, ras, RAS, RHO |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
| Summary | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. provided by RefSeq, Jul 2008 Transcript Variant: This variant (b) is composed of five exons and lacks exon 4a which the longer transcript variant (a) includes. This predominant variant (b) has a cds that terminates in exon 4b and encodes isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004985" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201958 | KRAS (Myc-DDK-tagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b | 10 ug |
$289.00
$450.00
|
|
| RC201958L1 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC201958L2 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged | 10 ug |
$750.00
|
|
| RC201958L3 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC201958L4 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged | 10 ug |
$750.00
|
|
| RG201958 | KRAS (tGFP-tagged) - Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b | 10 ug |
$489.00
$650.00
|
|
Citations
Signaling by RasG12V depends on EGFR activity in vivo
Vega-Cuesta, Lopez-Varea, Ruiz-Gomez
bioRxiv (2025)
Vega-Cuesta, Lopez-Varea, Ruiz-Gomez
bioRxiv (2025)
AI Summary: OriGene Technologies' human KRASB cDNA (SC109374) was used to generate constructs for studying the role of RasG12V signaling in vivo in this paper.
Snippet: Cas9 flies, Ras1 cDNA (RE53955, DGCR) and human KRASB cDNA (SC109374, OriGene Technologies) were cloned into reintegration vectors (RIVwhiteand RIVFRTMCSFRTMCS3) (Baena-Lopez et al, 2013) in either wild-type or G12V mutant forms and tagged or not with the Flag or HA epitopes at their 5’ end. All Ras1 knock-in constructs incl
A two-residue nascent-strand steric gate controls synthesis of 2'-O-methyl- and 2'-O-(2-methoxyethyl)-RNA
Freund, Taylor, Arangundy-Franklin et al
Nat Chem (2022) 15 (1), 91-100 DOI: 10.1038/s41557-022-01050-8
Freund, Taylor, Arangundy-Franklin et al
Nat Chem (2022) 15 (1), 91-100 DOI: 10.1038/s41557-022-01050-8
Cited by 63
AI Summary: OriGene products 6-XL6 (SP6 promoter, cat. no. SC109374) and pCMV6-XL5 (T7 promoter, cat. no. SC107921) were used as plasmid templates for mutagenesis to generate KRAS and CTNNB1 variants in this study.
Snippet: …SC109374) and pCMV6-XL5 (T7 promoter) (cat. … SC107921), respectively, were obtained from OriGene, USA. Site-directed mutagenesis was performed using a QuikChange II kit (Agilent Technologies, USA), according to the manufacturer’s protocol;KRAS mutations G12D (c.35 G A) and G13D (c.38 G G), and CTNNB1 mutation S33Y (c.98 C A) were introduced using primer sets shown in Supplementary Table 2 (‘Quik_KRAS_G12D_Fw/Rev’, ‘Quik_KRAS_G13D_Fw/Rev’ or ‘Quik_C
A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing
Taylor, Wan, Donde et al
Nat Chem (2022) 14 (11), 1295-1305 DOI: 10.1038/s41557-022-01021-z
Taylor, Wan, Donde et al
Nat Chem (2022) 14 (11), 1295-1305 DOI: 10.1038/s41557-022-01021-z
Cited by 66
AI Summary: OriGene’s human KRAS ORF cDNA clone (plasmid pCMV6-XL6, Cat. No. SC109374) was used as a template to generate full-length KRAS RNA transcripts for in vitro cleavage and gene-silencing experiments in this study.
Snippet: _KRas_ORF”) were using prepared using an SP6 transcription kit (NEB, USA) according to the manufacturer’s instructions, using a human KRAS ORF cDNA clone (in plasmid pCMV6-XL6)(Cat. … SC109374, Origene, USA) linearized with XmaI (NEB, USA) as template, and a FITC-AG transcription starter (IBA Life Sciences, Germany). Mutations c.35G>A [G12D] or c.38G>A [G13D] were introduced into the KRAS pl;Long (2.1 kb) 5′-fluorophore-labelled RNAs encoding the complete (wild-type) KRAS ORF and UTRs (‘Sub_KRas_ORF’) were prepared using an SP6 transcription kit (NEB) according to the manufacturer’s instructions, using a human KRAS ORF cDNA clone (in plasmid pCMV6-XL6;cat. … SC109374, Origene) linearized with XmaI (NEB) as template, and a FITC-AG transcription starter (IBA Life Sciences). …
Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange
Guillard, Kolasinska-Zwierz, Debreczeni et al
Nat Commun (2017) 8, 16111 DOI: 10.1038/ncomms16111
Guillard, Kolasinska-Zwierz, Debreczeni et al
Nat Commun (2017) 8, 16111 DOI: 10.1038/ncomms16111
AI Summary: OriGene’s wild-type K-Ras plasmid (SC109374) was used to transfect HEK293 cells, enabling the study of DARPin K27’s inhibitory effects on Ras nucleotide exchange in this research.
Snippet: Adherent HEK293 (Ad293) cells were dual transfected with a wild-type K-Ras encoding plasmid (Origene SC109374) and pcDNA3.1 vectors expressing FLAG-tagged DARPins
Differentially expressed nucleolar transforming growth factor-ß1 target (DENTT) exhibits an inhibitory role on tumorigenesis
Kandalaft, Zudaire, Portal-Núñez et al
Carcinogenesis (2008)
Kandalaft, Zudaire, Portal-Núñez et al
Carcinogenesis (2008)
AI Summary: In the paper "Differentially expressed nucleolar transforming growth factor-ß1 target (DENTT) exhibits an inhibitory role on tumorigenesis," OriGene products—including the DENTT cDNA clone (RC203307), DENTT shRNA plasmids (TG312799), and DENTT antibodies (TA500606)—were used for gene overexpression, knockdown, and protein detection experiments to investigate DENTT's function in cancer cell models.
Showing 1-5 of 5 papers.
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