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KRAS (NM_004985) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC201958
KRAS (Myc-DDK-tagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$289.00 $450.00
5 Days*
Bulk Requests & Clone Modifications

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Other products for "KRAS or NM_004985"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRAS
Synonyms C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201958 representing NM_004985
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAA
TTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGATTCCTACAGGAAGCAAGTAGTAATTGA
TGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAG
TACATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTC
ACCATTATAGAGAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAA
ATGTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCT
TTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATACATTAGTTCGAGAAATTC
GAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGAAAAAGAAGTCAAAGACAAAGTGTGTAAT
TATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201958 representing NM_004985
Red=Cloning site Green=Tags(s)

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ
YMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIP
FIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004985
ORF Size 564 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004985.5
RefSeq Size 5312 bp
RefSeq ORF 567 bp
Locus ID 3845
UniProt ID P01116, A0A024RAV5
Cytogenetics 12p12.1
Domains RAB, ras, RAS, RHO
Protein Families Druggable Genome
Protein Pathways Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
MW 21.2 kDa
Summary This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. provided by RefSeq, Jul 2008
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"NM_004985" in other vectors (6)
SKU Description Size Price
RC201958L1 Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged 10 ug
$750.00
RC201958L2 Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged 10 ug
$750.00
RC201958L3 Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged 10 ug
$750.00
RC201958L4 Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged 10 ug
$750.00
RG201958 KRAS (tGFP-tagged) - Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b 10 ug
$489.00 $650.00
SC109374 KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b 10 ug
$450.00

Plasmid Map for RC201958

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201958
Circular map for RC201958

file-alt Citations

Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer
Woolston, Khan, Spain et al
Cancer Cell (2019) 36 (1), 35-50.e9 DOI: 10.1016/j.ccell.2019.05.013
AI Summary: OriGene’s KRAS (NM_004985) Human Tagged ORF Clone (Cat# RC201958) was used in this study to investigate the molecular mechanisms underlying therapy resistance in colorectal cancer during anti-EGFR treatment.
Snippet: 70299 RRID:Addgene_70299 R777_E087-Hs.FGFR3 Addgene Cat# 70371 RRID:Addgene_70371 pDONOR223_BRAF_p.D594H Addgene Cat# 82816 RRID:Addgene_82816 KRAS (NM_004985) Human Tagged ORF Clone OriGene Cat# RC201958 pENTR1A Invitrogen Cat# A10462 pLX304 Addgene #25890 RRID:Addgene_25890 pLenti-CMV-Puro-DEST Addgene Cat# 17452 RRID:Addgene_17452 pEZY3 Addgene Cat# 18;... 70299 RRID:Addgene_70299 R777_E087-Hs.FGFR3 Addgene Cat# 70371 RRID:Addgene_70371 pDONOR223_BRAF_p.D594H Addgene Cat# 82816 RRID:Addgene_82816 KRAS (NM_004985) Human Tagged ORF Clone OriGene Cat# RC201958 pENTR1A Invitrogen Cat# A10462 pLX304 Addgene #25890 RRID:Addgene_25890 pLenti-CMV-Puro-DEST Addgene Cat# 17452 RRID:Addgene_17452 pEZY3 Addgene Cat# 18
FigureFigureFigure
KRAS G12D or G12V mutation in human brain arteriovenous malformations
Oka, Kushamae, Aoki et al
World Neurosurg (2019)
AI Summary: OriGene’s pCMV6-entry plasmids harboring KRAS wild type, G12D, or G12V were used as control reagents in transfected HEK293 cells to validate mutation detection and immunohistochemistry in the study.
Snippet: Japan). HEK293 cells transfected with pCMV6-entry plasmid harboring KRAS wild type (codon 35 G), G12D (codon 35 A) or G12V (codon 35 T) (OriGene, Rockville, MD) was used as a control experiment. In immunohistochemical
High expression of RAD51 promotes DNA damage repair and survival in KRAS-mutant lung cancer cells
Hu, Zhang, Zhao et al
BMB Rep (2019) 52 (2), 151-156
AI Summary: OriGene's pCMV6-Myc-DDK-tagged-KRAS vector was used as the source for cloning wild-type KRAS mRNA, enabling the study of KRAS's role in regulating RAD51 expression and DNA damage repair in lung cancer cells.
Snippet: The human wild-type KRAS mRNA sequence was cloned from the pCMV6-Myc-DDK-tagged-KRAS vector (OriGene Technologies Co., Rockville, MD, USA) into the 3XFLAG-CMV vector to create FLAG-KRAS constructs. G13D mutation was generated by site-directed mutagenesis kit (Qiagen Co., Hilden, Germany)
FigureFigureFigure
Characterizing the interaction between insulin-like growth factor 2 mRNA-binding protein 1 (IMP1) and KRAS expression
Mackedenski, Wang, Li et al
Biochem. J. (2018)
AI Summary: OriGene contributed to this study by providing the human KRAS cDNA clone (accession # NM_033360), which was used as a DNA template for in vitro experiments characterizing the interaction between IMP1 and KRAS mRNA.
Snippet: Generation of DNA templates and radiolabeled in-vitro transcription The human KRAS cDNA clone which contains the full coding sequence and a partial 3' untranslated region (accession # NM_033360) was purchased from Origene Technologies Inc. (Rockville, MD
Mutant KRAS promotes liver metastasis of colorectal cancer, in part, by upregulating the MEK-Sp1-DNMT1-miR-137-YB-1-IGF-IR signaling pathway
Chu, Lin, Wu et al
Oncogene (2018)
AI Summary: OriGene products contributed to this study by providing full-length human KRAS, Sp1, and YB-1 cDNA clones, which were used to investigate the molecular mechanisms underlying KRAS-driven colorectal cancer metastasis.
Snippet: …Oncotarget. 2016;7:2709–20. * * PubMed [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=26646451] * Google Scholar [;Full-length KRAS, Sp1, and YB-1 human complementary DNA clones were purchased from OriGene Technologies and subcloned into the HindIII/BglII, EcoRI/XbaI, and BglII/XbaI sites, respectively, of p3xFLAG-CMV26 expression vector (Sigma-Aldrich)
MicroRNA-622 is a novel mediator of tumorigenicity in melanoma by targeting Kirsten rat sarcoma
Dietrich, Kuphal, Spruss et al
Pigment Cell Melanoma Res (2018)
AI Summary: OriGene’s pCMV6-Entry KRAS cDNA vector ("ORF-KR") was used to overexpress KRAS in melanoma cell lines, enabling functional studies of the miR-622-KRAS pathway in tumorigenicity.
Snippet: KRAS "rescue" plasmid assays A pCMV6-Entry cDNA vector system was used containing open-reading-frame of KRAS ("ORF-KR", OriGene Technologies, Rockville, USA) for KRAS overexpression in melanoma cell lines. An empty pCMV6 vector was used as negative
Showing 1-6 of 10 papers.
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