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POLE4 (NM_019896) Human Untagged Clone

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SKU
SC108865
POLE4 (untagged)-Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4)
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol POLE4
Synonyms p12; YHHQ1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_019896, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCGGCGGCGGCGGCAGGAAGCGGGACGCCCCGAGAGGAGGAGGGACCTGCTGGGGAGGCAGCGG
CCTCGCAGCCCCAGGCCCCAACGAGTGTGCCTGGGGCTCGTCTCTCGAGGTTGCCTCTGGCGCGAGTGAA
GGCCTTGGTGAAGGCAGATCCCGACGTGACGCTAGCGGGACAGGAAGCCATCTTCATTCTGGCACGAGCC
GCGGAACTGTTTGTGGAGACCATTGCAAAAGATGCCTACTGTTGCGCTCAGCAGGGAAAAAGGAAAACCC
TTCAGAGGAGAGACTTGGATAATGCAATAGAAGCTGTGGATGAATTTGCTTTTCTGGAAGGTACTTTAGA
TTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_019896 unedited
GGGTCAGGATTAGTATACGATTACTATAGGCGGCCGCGCAATTCGCACGAGGCGCAGCAC
GTCTCAAGCCGGGATGGCGGCGGCGGCGGCGGCAGGAAGCGGGACGCCCCGAGAGGAGGA
GGGACCTGCTGGGGAGGCAGCGGCCTCGCAGCCCCAGGCCCCAACGAGTGTGCCTGGGGC
TCGTCTCTCGAGGTTGCCTCTGGCGCGAGTGTTTGCCTTGGTGAAGGCAGATCCCGACGT
GACGCTAGCGGGACAGGAAGCCATCTTCATTCTGGCACGAGCCGCGGAACCTGTTTGTGG
AGACCATTGCAAAAGATGCCTACTGTTGCGCTCAGCAGGGAAAAAGGAAAACCCTTCAGA
GGAGAGACTTGGATAATGCAATAGAAGCTGTGGATGAATTTGCTTTTCTGGAAGGTACTT
TAGATTGATTGCCGAGCGGGGCAGTTTTGTGAGCCTTCATCTGAAGCCTTCAGTTCACCC
CTCTGCACAGGCCTCAGCTTTGAAGAACGGAGTCTTTGCACTTACACACACTCTTCCTGT
TCTGCCTTCACCTATGCCGGGATAAGCAGAGATCTCATCAATTAGCTCTTCTCTGCAAGG
TCTTCCACTGTTTCTGTCTGTCTTCCATATCAAGCCCTGGATGCAGCTGCTGCTGCTTAC
AGCAGAGATGAAGAAAGTGTTCTGCATAAGTGGCTTCCTTGATGATGACGACCAGAATAA
AGGTTTTTGATCCACCCTCAAAAAAAAAACAAAAACTCGACTCTAAATTGCGGCCGCGTC
ACTAGTTGTTTCCTGAACAGATCCCGGGTGGCATTCCTGTGACCCCTCCCCAATGCCTCT
CCTTGGCCTTGAAGTTGCCCCTCCTTGCCCACCACCCTGGGCCTAATAAATAAGTTGCAT
CAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_019896 unedited
ACCGCGGCCGCCGTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGTTGATCAAAAACC
TTTATTCTGGTCCTCATCATTCAGGAAGCCACTTATGCAGAACACTTTCTTCATCTCTGC
TCTAAGCAGCAGCAGCTGCATCCAGGCTTGATATGGAAGACAGACAGAAACAGTGGAAGA
CCTTGCAGAGAAGAGCTAATTGATGAGATCTCTGCTTATCCCGGCATAGGTGAAGGCAGA
ACAGGAAGAGTGTGTGTAAGTGCAAAGACTCCGTTCTTCAAAGCTGAGGCCTGTGCAGAG
GGGTGAACTGAAGGCTTCAGATGAAGGCTCACAAAACTGCCCCGCTCGGCAATCAATCTA
AAGTACCTTCCAGAAAAGCAAATTCATCCACAGCTTCTATTGCATTATCCAAGTCTCTCC
TCTGAAGGGTTTTCCTTTTTCCCTGCTGAGCGCAACAGTAGGCATCTTTTGCAATGGTCT
CCACAAACAGTTCCGCGGCTCGTGCCAGAATGAAGATGGCTTCCTGTCCCGCTAGCGTCA
CGTCGGGATCTGCCTTCACCAAGGCCTTCACTCGCGCCCAGAGGCAACCTCGAGAGACGA
GCCCCAGGCACACTCGTTGGGGCCTGGGGCTGCGAGGCCGCTGCCTCCCAGCAGGGTCCT
CCTCCTCTCGGGGCGTCCCGGTTTCTGGCGGCGCCGCCGGCGGCATTCCGGGCTTTGAGC
GTGCTTGGGCCTCGTGCCGAATTCCGGGGCCGCCCTTTATTTGAGTTGTATTACAAAAAT
TTGACGGTTCACTAAACAAACTTTGGTTTTTAGAACTTCCACGGTCACCCCTACGGCCTT
TTGGTTAACGGGGCGGGGGTTATTACCACACTTGGGACACCCCGCTGATTTTGGGCCAAA
AAAAACTCCCTTTGG
Restriction Sites NotI-NotI |
ACCN NM_019896
Insert Size 680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019896.2, NP_063949.2
RefSeq Size 710 bp
RefSeq ORF 354 bp
Locus ID 56655
UniProt ID Q9NR33
Cytogenetics 2p12
Domains CBFD_NFYB_HMF
Protein Pathways Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
Summary POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.supplied by OMIM, Apr 2004
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Documents
Resources
"NM_019896" in other vectors (4)
SKU Description Size Price
RC207681 POLE4 (Myc-DDK-tagged)-Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) 10 ug
$150.00
RC207681L3 Lenti ORF clone of Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), Myc-DDK-tagged 10 ug
$450.00
RC207681L4 Lenti ORF clone of Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), mGFP tagged 10 ug
$450.00
RG207681 POLE4 (tGFP-tagged) - Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) 10 ug
$489.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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