POLE4 (NM_019896) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207681L3
Lenti ORF clone of Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), Myc-DDK-tagged
  $450.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POLE4
Synonyms p12; YHHQ1
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207681).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_019896
ORF Size 351 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019896.2
RefSeq Size 710 bp
RefSeq ORF 354 bp
Locus ID 56655
UniProt ID Q9NR33
Cytogenetics 2p12
Domains CBFD_NFYB_HMF
Protein Pathways Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
MW 12.3 kDa
Summary POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.supplied by OMIM, Apr 2004
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Documents
Resources
"NM_019896" in other vectors (4)
SKU Description Size Price
RC207681 POLE4 (Myc-DDK-tagged)-Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) 10 ug
$150.00
RC207681L4 Lenti ORF clone of Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), mGFP tagged 10 ug
$450.00
RG207681 POLE4 (tGFP-tagged) - Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) 10 ug
$489.00
SC108865 POLE4 (untagged)-Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) 10 ug
$150.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.