NDUFAF1 (NM_016013) Human Untagged Clone

SKU SC108715

NDUFAF1 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1 (NDUFAF1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDUFAF1
• Synonyms: CGI-65; CGI65; CIA30; MC1DN11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108715 sequence for NM_016013 edited (data generated by NextGen Sequencing); ATGGCTTTGGTTCACAAATTGCTGCGTGGTACTTATTTTCTCAGAAAATTCTCTAAGCCA; ACTTCTGCCTTGTATCCATTTTTGGGTATTCGCTTTGCAGAGTATTCCAGTAGTCTTCAG; AAACCAGTGGCTTCTCCTGGCAAAGCCTCCTCACAGAGGAAGACTGAAGGGGATTTGCAA; GGAGATCACCAGAAAGAAGTTGCTTTGGATATAACTTCTTCTGAGGAGAAGCCTGATGTT; AGTTTCGATAAAGCAATTAGAGATGAAGCAATATACCATTTTAGGCTTTTGAAGGATGAA; ATTGTGGATCATTGGAGAGGACCGGAAGGCCACCCTCTGCATGAGGTCTTGCTGGAACAA; GCCAAGGTTGTCTGGCAATTCCGGGGGAAAGAAGATTTGGATAAGTGGACAGTGACTTCT; GATAAGACGATTGGAGGCAGAAGTGAAGTGTTTTTGAAAATGGGCAAGAATAACCAAAGT; GCACTGCTATATGGAACTCTGAGCTCTGAGGCGCCTCAGGACGGGGAGTCTACCCGAAGT; GGGTACTGTGCAATGATATCCAGGATTCCAAGGGGTGCTTTTGAGAGGAAGATGTCTTAC; GATTGGTCCCAGTTCAATACTCTGTATCTCCGTGTACGTGGGGATGGTCGGCCTTGGATG; GTGAATATCAAGGAGGACACAGATTTCTTCCAGAGGACGAATCAGATGTATAGTTACTTC; ATGTTCACCCGCGGGGGACCCTACTGGCAGGAGGTCAAGATTCCTTTTTCCAAATTTTTC; TTCTCTAATCGAGGAAGAATCCGGGATGTTCAGCATGAGCTTCCGCTTGATAAGATCTCT; TCTATAGGATTCACCTTGGCTGATAAAGTGGATGGTCCATTCTTCCTGGAGATAGATTTT; ATTGGCGTGTTTACTGATCCAGCTCATACAGAAGAATTTGCCTATGAAAATTCTCCAGAG; CTTAACCCAAGGCTTTTTAAATAA; ; Clone variation with respect to NM_016013.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016013 unedited; TCACCATTTTGTAATACGACTTACTATAGGGCGGCCCGCGAATTCGCACGAGGGCTCGGG; GAGGTAAGCGGCGGCAGGCCGGCGGTTGGTGTGTCCCGGGTGTGGGGAGGCGACAGAGCC; CTGGCACTTGAGGGTTGAGGGGGCCTCCCCAGCGCGGCGAACCGTCTAGCCTCCGGAGGC; CAGGCCGTGAGTGCGGGAGGTATACGCCAAGGCGGAAGAATTTTGCCACTCACTACCTGT; GTGACCTCGGGTAAATTAGCCTTGGAACGTCAGTTTCTTCGTCTCTATAATTGAAATAAT; AATAGTACCTCTCTCAGGATTGTTGTGAGCCGTCAGTGAAACACTTAGAGCAGTTTCTGG; CACATGGTAGAATTGGGCTATTTGCTGAAGCTTCTTGGTGGCCCTTGCTAGCCCAGGAAG; AAACTTACATTTTGATTTTTTTGTACCATGGCTTTGGTTCACAAATTGCTGCGTGGTACT; TATTTTCTCAGAAAATTCTCTAAGCCAACTTCTGCCTTGTATCCATTTTTGGGTATTCGC; TTTGCAGAGTATTCCAGTAGTCTTCAGAAACCAGTGGCTTCTCCTGGCAAAGCCTCCTCA; CAGAGGAAGACTGAAGGGGATTTGCAAGGAGATCACCAGAAAGAAGNTGCTTTGGATATA; ACTTCTTCTGANGAGAAGCCTGATGCTAGTTTCGATAAAGCAATTAGAGATGAAGCCATA; TACCATTTTANGCTTTTGAAAGATGAAATTGTGGATCATTGGAGAGGACGGNAAGCCACC; CTCTGCATGANGTCCTGCTGGAACAGCCCAAGTTGTCTGGCCATTCCGGGGGAAGAAAGA; TTGCATAATGGACAGTGACTTCTGATAAAACAATTGGAAGCACAAATGAAATGTTTTTGG; AAATGGCCAGAATACCCAAGGGCCCTGCTTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016013 unedited; NCCTGTCTTGAAGAAAATTTTATTTTGTCTATATCATTGTAGCAGCTAGTACCCTTAGAT; TAGTAAAACAAAACTACCATATGATCTTTATTTAAAAAGCCTTGGGTTAAGCTCTGGAGA; ATTTTCATAGGCAAATTCTTCTGTATGAGCTGGATCAGTAAACACGCCAATAAAATCTAT; CTCCAGGAAGAATGGACCATCCACTTTATCAGCCAAGGTGAATCCTATAGAAGAGATCTT; ATCAAGCGGAAGCTCATGCTGAACATCCCGGATTCTTCCTCGATTAGAGAAGAAAAATTT; GGAAAAAGGAATCTTGACCTCCTGCCAGTAGGGTCCCCCGCGGGTGAACATGAAGTAACT; ATACATCTGATTCGTCCTCTGGAAGAAATCTGTGTCCTCCTTGATATTCACCATCCAAGG; CCGACCATCCCCACGTACACGGAGATACAGAGTATTGAACTGGGACCAATCGTAAGACAT; CTTCCTCTCAAAAGCACCCCTTGGAATCCTGGATATCATTGCACAGTACCCACTTCGGGT; AGACTCCCCGTCCTGAGGCGCCTCAGAGCTCAGAGTTCCATATAGCAGTGCACTTTGGTT; ATTCTTGCCCATTTTCAAAAACACTTCACTTCTGCCTCCAATCGTCTTATCAGAAGTCAC; TGTCCACTTATCCAAATCTTCTTTCCCCCGGAATTGCCAGACAACCTTGGCTTGTTCCAG; CAAGACCTCATGCAGAGGGTGGCCTTNCGGTCCTCTCCATGATCCACAATTTCATCCTTC; AAAGCCTAAAATGGTATATTGCTTCATCTCTAATTGGCTTATCGAAACTAACATCAGGCT; TCTCCTCAGAAGAAGTATATCCAAAGCAACTTCTTTCTGGTGATCTNCTTGCAAATCCCC; TTCAGTCTTTCTCTGTGAAGGAGCCTTGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_016013
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016013.2, NP_057097.2
• RefSeq Size: 1474 bp
• RefSeq ORF: 984 bp
• Locus ID: 51103
• UniProt ID: Q9Y375
• Cytogenetics: 15q15.1
• Summary: This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011]; Transcript Variant: This variant (1) represents the predominant transcript and is protein-coding.