ZNHIT2 (NM_014205) Human Untagged Clone

SKU SC108656

ZNHIT2 (untagged)-Human zinc finger, HIT-type containing 2 (ZNHIT2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ZNHIT2
• Synonyms: C11orf5; FON
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108656 sequence for NM_014205 edited (data generated by NextGen Sequencing); ATGGAGCCGGCCGGGCCCTGTGGCTTCTGCCCGGCGGGGGAGGTCCAGCCAGCGCGTTAC; ACCTGCCCTCGCTGTAATGCGCCCTACTGCTCGCTGCGCTGCTACCGGACGCATGGCACC; TGCGCAGAAAACTTCTACCGTGACCAGGTGCTGGGAGAGCTCCGCGGTTGCAGCGCTCCT; CCCAGCCGCCTAGCAAGCGCCCTACGCCGGCTGCGTCAGCAACGCGAGACCGAGGACGAG; CCTGGGGAAGCAGGCCTCAGCTCCGGCCCGGCGCCCGGCGGCCTATCGGGACTCTGGGAG; CGGCTGGCGCCGGGCGAAAAAGCTGCCTTCGAGCGGCTGCTGAGCCGCGGTGAGGCCGGG; CGGCTGCTGCCTCCATGGCGGCCGTGGTGGTGGAACCGCGGAGCCGGACCGCAGCTTCTG; GAGGAGCTGGATAATGCCCCGGGTAGTGACGCCGCGGAGCTGGAGCTCGCCCCTGCGAGG; ACCCCGCCGGATTCTGTGAAAGATGCCTCCGCCGCGGAGCCCGCGGCCGCCGAGCGGGTT; CTTGGAGATGTCCCGGGGGCCTGCACGCCCGTCGTACCCACCCGCATCCCCGCGATAGTC; AGCCTGAGCCGCGGCCCAGTCTCGCCGCTCGTGCGCTTCCAGCTGCCCAATGTGCTGTTC; GCCTACGCGCATACTCTCGCCCTGTATCACGGCGGTGACGACGCGCTGCTCTCTGACTTC; TGTGCCACACTGCTCGGCGTTTCCGGAGCCCTGGGTGCCCAGCAAGTCTTCGCCTCTGCG; GAAGAAGCCCTGCAGGCGGCAGCCCACGTGCTGGAAGCAGGCGAGCACCCGCCGGGGCCC; CTGGGCACACGAGGGGCTATGCACGAGGTCGCCCGCATCCTGCTGGGCGAGGGCCCGACC; AACCAGAAAGGCTACACGCTGGCAGCACTGGGGGACCTGGCACAGACCCTGGGCCGTGCC; CGGAAACAGGCCGTGGCTAGAGAAGAGCGAGATCATCTTTACCGGGCCCGGAAAAAGTGC; CAGTTCCTCCTGGCCTGGACCAACGAAAATGAGGCGGCCCTCACACCCCTGGCTCTAGAC; TGCGCCAGGGCTCACCAAGCCCATGCTGTGGTAGCCGAGGAGGTGGCCGCCCTCACTGGG; GAGCTGGAGCGGCTTTGGGGAGGCCCCGTGCCACCTGCCCCAAGAACTCTCATTGAGGAG; CTCCCTAGCTGA; ; Clone variation with respect to NM_014205.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014205 unedited; TATAGGCGGCCGGCGATTCGGCACGAGGCCTCGTGCCGAATTCGGCACCAGGCTACCACC; GGCGTGCGTTCGCAGGTAGATCGCGGTGCCAGTTGCCATGGAGCCGGCCGGGCCCTGTGG; CTTCTGCCCGGCGGGGGAGGTCCAGCCAGCGCGTTACACCTGCCCTCGCTGTATCTGCGC; CCTACTGCTCGCTGCGCTGCTACCGGACGCATGGCACCTGCGCAGAAAACTTCTACCGTG; ACCAGGTGCTGGGAGAGCTCCCTGGTTGCATCCCTCCTCCCAGCCGCCTAGCAAGCGCCC; TACGCCGGCTGCGTCAGCAACGCGAGACCGAGGACGAGCCTGGGGAAGCAGGCATCAGCT; CCGGCCCGGCGCCCGGCGGCCTATCGGGACTCTGGGAGCGGCTGGCGCCGGGCGAAAAAG; CTGCCTTCGAGCGGCTGACGAGCCCCGGTGAGGCCGGGCGGCTGCTGCCTCCATGGCGGC; CGTGGTGGTGGAACCGCGGAGCCGGAACGTAGCTTCTGGAGGAGCTGATATAATGCCCCC; GGTAGTGACGCCGCGGAGCTGGAGCTCGCCCCTGCGACGCTCCCGCCGGATTCTGTGAAA; GATGCCTCCGCCGCGGAGCCCGCGAACGCCGAGCGGGTTCTTGGAGATGTCCCGGGGGGC; CTGCACGCCCGTCGTACCCACCCGCATCCCCGCGATAGTCAGCCTGAGCCGCGGCCCAGT; CTCGCCTGTCGTGCGCTTCCAGCTGCCCAATGTGCTGTTCGCCTACGCGCATACTCTCGC; CCTGTATCCCCGCGGTGACGACGCGCTGCTCTCTGACTTTTGTGCCCACTGCTCGGAGGT; TCCGGAGCCCTGGTGCCCACCAAGTCTCCTCTCTGCAGGAGAGCCCTGCGGCGGCCACAC; CGTGCCTGGAGCAGCGAGCCCCCCGCGGGACTCTGGCACCCAGGGGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014205 unedited; AAGTAAAGGNCCCGACCAGCTTTATAATGACCAGGTAACCCGTTCAGCTAGGGAGCTCCT; CATTTTGAGTTCTTGGGGCAGGTGGCACGGGGCCTCCCCAAAGCCGCTCCAGCTCCCCAG; TGAGGGCGGCCACCTCCTCGGCTACCACAGCATGGGCTTGGTGAGCCCTGGCGCAGTCTA; GAGCCAGGGGTGTGAGGGCCGCCTCATTTTCGTTGGTCCAGGCCAGGAGGAACTGGCACT; TTTTCCGGGCCCGGTAAAGATGATCTCGCTCTTCTCTAGCCACGGCCTGTTTCCGGGCAC; GGCCCAGGGTCTGTGCCAGGTCCCCCAGTGCTGCCAGCGTGTAGCCTTTCTGGTTGGTCG; GGCCCTCGCCCAGCAGGATGCGGGCGACCTCGTGCATAGCCCCTCGTGTGCCCAGGGGCC; CCGGCGGGTGCTCGCCTGCTTCCAGCACGTGGGCTGCCGCCTGCAGGGCTTCTTCCGCAG; AGGCGAAGACTTGCTGGGCACCCAGGGCTCCGGAAACGCCGAGCAGTGTGGCACAGAAGT; CAGAGAGCAGCGCGTCGTCACCGCCGTGATACAGGGCGAGAGTATGCGCGTAGGCGAACA; GCACATTGGGCAGCTGGAAGCGCACGAGCGGCGAGACTGGGCCGCCGCTCACGCTGACTA; TCGCGGCGATGCGGGTGGGTACGACGGGCGTGCAGGCCCCCGGGACATCTTCCAGACCCC; GCTCGGCGGGCGCGGGCTCCGCGGCGGAGCATCCTTTACCAGATCCGGCGTGGTCCTCCG; AGGGCGAGCTCCAGCTCCGCGGCGTCCTTACCCGGGGCATATCCACTTCCTCACAAGCTG; CGGGCCCGCTCCCGGGTCCCCCCCCCGGCCGCCTGGGAGGAAAACCCGCCGGGCTTACCG; GGCTAAAAACCGTTCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014205
• Insert Size: 4780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014205.2, NP_055020.1
• RefSeq Size: 1321 bp
• RefSeq ORF: 1212 bp
• Locus ID: 741
• UniProt ID: Q9UHR6
• Cytogenetics: 11q13.1
• Domains: zf-HIT
• Summary: May act as a bridging factor mediating the interaction between the R2TP/Prefoldin-like (R2TP/PFDL) complex and U5 small nuclear ribonucleoprotein (U5 snRNP) (PubMed:28561026). Required for the interaction of R2TP complex subunit RPAP3 and prefoldin-like subunit URI1 with U5 snRNP proteins EFTUD2 and PRPF8 (PubMed:28561026). May play a role in regulating the composition of the U5 snRNP complex (PubMed:28561026).[UniProtKB/Swiss-Prot Function]